Enhanced rigid-bond restraints

An extension is proposed to the rigid-bond description of atomic thermal motion in crystals

RNA oligomers at atomic resolution containing 1‐methylpseudouridine, an essential building block of mRNA vaccines

All widely used mRNA vaccines against COVID‐19 contain in their sequence 1‐methylpseudouridine (m1Ψ) instead of uridine. In this publication, we report two high resolution crystal structures (at up to 1.01 and 1.32 Å, respectively) of one such double‐stranded 12‐mer RNA sequence crystallized in two crystal forms. The structures are compared with similar structures which do not contain this modification. Additionally, the X‐ray structure of 1‐methyl‐pseudouridine itself was determined

ANODE: anomalous and heavy-atom density calculation

The program ANODE determines anomalous (or heavy-atom) densities by reversing the usual procedure for experimental phase determination. Instead of adding a phase shift to the heavy-atom phases to obtain a starting value for the native protein phase, this phase shift is subtracted from the native phase to obtain the heavy-atom substructure phase

A multi-wavelength polarimetric study of the blazar CTA 102 during a Gamma-ray flare in 2012

We perform a multi-wavelength polarimetric study of the quasar CTA 102 during an extraordinarily bright $\gamma$-ray outburst detected by the {\it Fermi} Large Area Telescope in September-October 2012 when the source reached a flux of F$_{>100~\mathrm{MeV}} =5.2\pm0.4\times10^{-6}$ photons cm$^{-2}$ s$^{-1}$. At the same time the source displayed an unprecedented optical and NIR outburst. We study the evolution of the parsec scale jet with ultra-high angular resolution through a sequence of 80 total and polarized intensity Very Long Baseline Array images at 43 GHz, covering the observing period from June 2007 to June 2014. We find that the $\gamma$-ray outburst is coincident with flares at all the other frequencies and is related to the passage of a new superluminal knot through the radio core. The powerful $\gamma$-ray emission is associated with a change in direction of the jet, which became oriented more closely to our line of sight ($\theta\sim$1.2$^{\circ}$) during the ejection of the knot and the $\gamma$-ray outburst. During the flare, the optical polarized emission displays intra-day variability and a clear clockwise rotation of EVPAs, which we associate with the path followed by the knot as it moves along helical magnetic field lines, although a random walk of the EVPA caused by a turbulent magnetic field cannot be ruled out. We locate the $\gamma$-ray outburst a short distance downstream of the radio core, parsecs from the black hole. This suggests that synchrotron self-Compton scattering of near-infrared to ultraviolet photons is the probable mechanism for the $\gamma$-ray production.Comment: Accepted for publication in The Astrophysical Journa

DESIGN FOR CONTAINMENT TECHNIQUES TO REDUCE SPACECRAFT RE-ENTRY FOOTPRINT

Clean Space is an ESA initiative to address the technological challenges in reaching sustainability of space activities on Earth and in orbit. Its purpose is to guarantee the safety of the human population and future space activities. Under this initiative, different solutions, such as Design for Demise (D4D) and debris removal, are studied. Design for Containment (D4C) is the design of spacecraft systems using methods that keep harmful objects under control or within limits, so as to reduce the number of impacting fragments on ground during re-entry events. While potentially increasing the impact mass, the lower number of fragments may significantly reduce the overall casualty risk. These methods could render future un-controlled re-entry missions compliant to the space debris mitigation guidelines without major design changes, that could put them at cost or schedule risk. In this paper, the system level investigations, simulation results and first findings of the on-going ESA funded study "Containment Techniques to Reduce Spacecraft Re-Entry Footprint” (also called the D4C study) are presented. The study aims to identify and validate promising containment techniques and to provide an update to the current material database for re-entry models (ESTIMATE). Feedback to the ESA guidelines for demise verification (DIVE) will also be a valuable outcom

Haplotype differences for copy number variants in the 22q11.23 region among human populations: a pigmentation-based model for selective pressure.

Two gene clusters are tightly linked in a narrow region of chromosome 22q11.23: the macrophage migration inhibitory factor (MIF) gene family and the glutathione S-transferase theta class. Within 120 kb in this region, two 30-kb deletions reach high frequencies in human populations. This gives rise to four haplotypic arrangements, which modulate the number of genes in both families. The variable patterns of linkage disequilibrium (LD) between these copy number variants (CNVs) in diverse human populations remain poorly understood. We analyzed 2469 individuals belonging to 27 human populations with different ethnic origins. Then we correlated the genetic variability of 22q11.23 CNVs with environmental variables. We confirmed an increasing strength of LD from Africa to Asia and to Europe. Further, we highlighted strongly significant correlations between the frequency of one of the haplotypes and pigmentation-related variables: skin color (R2=0.675, P<0.001), distance from the equator (R2=0.454, P<0.001), UVA radiation (R2=0.439, P<0.001), and UVB radiation (R2=0.313, P=0.002). The fact that all MIF-related genes are retained on this haplotype and the evidences gleaned from experimental systems seem to agree with the role of MIF-related genes in melanogenesis. As such, we propose a model that explains the geographic and ethnic distribution of 22q11.23 CNVs among human populations, assuming that MIF-related gene dosage could be associated with adaptation to low UV radiatio

The Repeating Flaring Activity of Blazar AO 0235+164

Context. Blazar AO 0235+164, located at redshift z = 0.94, has undergone several sharp multi-spectral-range flaring episodes during the last decades. In particular, the episodes peaking in 2008 and 2015, that received extensive multi-wavelength coverage, exhibited interesting behavior. Aims. We study the actual origin of these two observed flares by constraining the properties of the observed photo-polarimetric variability, those of the broad-band spectral energy-distribution and the observed time-evolution behavior of the source as seen by ultra-high resolution total-flux and polarimetric Very-long-baseline interferometry (VLBI) imaging. Methods. The analysis of VLBI images allows us to constrain kinematic and geometrical parameters of the 7 mm jet. We use the Discrete Correlation Function to compute the statistical correlation and the delays between emission at different spectral ranges. Multi-epoch modeling of the spectral energy distributions allows us to propose specific models of emission; in particular for the unusual spectral features observed in this source in the X-ray region of the spectrum during strong multi spectral-range flares. Results. We find that these X-ray spectral features can be explained by an emission component originating in a separate particle distribution than the one responsible for the two standard blazar bumps. This is in agreement with the results of our correlation analysis that do not find a strong correlation between the X-rays and the remaining spectral ranges. We find that both external Compton dominated and synchrotron self-Compton dominated models can explain the observed spectral energy distributions. However, synchrotron self-Compton models are strongly favored by the delays and geometrical parameters inferred from the observations

Search for CP Violation in the Decay Z -> b (b bar) g

About three million hadronic decays of the Z collected by ALEPH in the years 1991-1994 are used to search for anomalous CP violation beyond the Standard Model in the decay Z -> b \bar{b} g. The study is performed by analyzing angular correlations between the two quarks and the gluon in three-jet events and by measuring the differential two-jet rate. No signal of CP violation is found. For the combinations of anomalous CP violating couplings, ${\hat{h}}_b = {\hat{h}}_{Ab}g_{Vb}-{\hat{h}}_{Vb}g_{Ab}$ and $h^{\ast}_b = \sqrt{\hat{h}_{Vb}^{2}+\hat{h}_{Ab}^{2}}$, limits of \hat{h}_b < 0.59$and$h^{\ast}_{b} < 3.02$ are given at 95\% CL.Comment: 8 pages, 1 postscript figure, uses here.sty, epsfig.st

Stable isotope and minor element proxies for Eocene climate of Seymour Island, Antarctica

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/94846/1/palo900.pd