Esponenziale di matrice: aspetti computazionali

Le funzioni di matrici giocano un ruolo importante in molte applicazioni. Tra le funzioni di matrici più studiate in analisi numerica vi è l'esponenziale di matrice che è fondamentale nell'ambito delle equazioni differenziali lineari. Vista l'importanza ricoperta dall'esponenziale di matrice, in questo testo verranno mostrate alcune sue proprietà, seguite da un importante metodo di calcolo e da altri algoritmi per il calcolo dell'esponenziale di matrice, mostrando con un esempio numerico le differenze tra i diversi metodi

Implications of Conflicting Associations of the Prion Protein (PrP) Gene with Scrapie Susceptibility and Fitness on the Persistence of Scrapie

Existing mathematical models for scrapie dynamics in sheep populations assume that the PrP gene is only associated with scrapie susceptibility and with no other fitness related traits. This assumption contrasts recent findings of PrP gene associations with post-natal lamb survival in scrapie free Scottish Blackface populations. Lambs with scrapie resistant genotypes were found to have significantly lower survival rates than those with susceptible genotypes. The present study aimed to investigate how these conflicting PrP gene associations may affect the dynamic patterns of PrP haplotype frequencies and disease prevalence.A deterministic mathematical model was developed to explore how the associations between PrP genotype and both scrapie susceptibility and postnatal lamb mortality affect the prevalence of scrapie and the associated change in PrP gene frequencies in a closed flock of sheep. The model incorporates empirical evidence on epidemiological and biological characteristics of scrapie and on mortality rates induced by causes other than scrapie. The model results indicate that unfavorable associations of the scrapie resistant PrP haplotypes with post-natal lamb mortality, if sufficiently strong, can increase scrapie prevalence during an epidemic, and result in scrapie persisting in the population. The range of model parameters, for which such effects were observed, is realistic but relatively narrow.The results of the present model suggest that for most parameter combinations an unfavourable association between PrP genotype and post-natal lamb mortality does not greatly alter the dynamics of scrapie and, hence, would not have an adverse impact on a breeding programme. There were, however, a range of scenarios, narrow, but realistic, in which such an unfavourable association resulted in an increased prevalence and in the persistence of infection. Consequently, associations between PrP genotypes and fitness traits should be taken into account when designing future models and breeding programmes

Synthesis of rhodamine B ?€" benzenesulfonamide conjugates and their inhibitory activity against human ?- and bacterial/fungal ?-carbonic anhydrases

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Carbonic anhydrase inhibitor coated gold nanoparticles selectively inhibit the tumor-associated isoform IX over the cytosolic isozymes I and II.

International audienc

Survey in radiation oncology departments in Germany, Austria, and Switzerland: state of digitalization by 2023.

PURPOSE The aim of this work was to assess the current state of digitalization in radiation oncology departments in Germany, Austria, and Switzerland. METHODS A comprehensive survey was conducted in a digital format, consisting of 53 questions that covered various aspects of digitalization including patient workflow, departmental organization, radiotherapy planning, and employee-related aspects. RESULTS Overall, 120 forms were eligible for evaluation. Participants were mainly physicians or medical physicists responsible for digitalization aspects in their departments. Nearly 70% of the institutions used electronic patient records, with 50% being completely paperless. However, the use of smartphone apps for electronic patient reported outcomes (ePROMs) and digital health applications (DIGA) was limited (9% and 4.9%, respectively). In total, 70.8% of the radio-oncology departments had interfaces with diagnostic departments, and 36% had digital interchanges with other clinics. Communication with external partners was realized mainly through fax (72%), e‑mails (55%), postal letters (63%), or other digital exchange formats (28%). Almost half of the institutions (49%) had dedicated IT staff for their operations. CONCLUSION To the best of our knowledge, this survey is the first of its kind conducted in German-speaking radiation oncology departments within the medical field. The findings suggest that there is a varied level of digitalization implementation within these departments, with certain areas exhibiting lower rates of digitalization that could benefit from targeted improvement initiatives

Using ABM to explore the role of socio-environmental interactions on Ancient Settlement Dynamics

This paper presents a work in progress within the project « Modeling the role of socio-environmental interactions on Ancient Settlement Dynamics - ModelAnSet » developed by archaeologists, historians, palaeoenvironmentalists and computer scientists at University Côte d’Azur (Nice). Agent-based modelling is used to explore the respective role of environmental and social factors in the evolution of the settlement pattern and dynamics during the Roman period in South-Eastern France. The model aims at simulating the impact of the climatic and macro-economic conditions on the behaviour of Gallo-Roman landowners. According to the profit they derive from their farms and/or villas, which depends both on natural and socio-economic factors, the landowners can decide to maintain without change, improve, enlarge or abandon their agricultural holdings or to create a new one. Through the repeated landowners’ decision-making, the ABM thus simulates a changing macro-level settlement pattern, in terms of number, type and location of the settlements. The paper focuses on the conceptual model in order to present the model entities and the dynamics underlying their interactions, and explain our choices and hypotheses

Low concentrations of nitric oxide modulate Streptococcus pneumoniae biofilm metabolism and antibiotic tolerance

Streptococcus pneumoniae is one of the key pathogens responsible for otitis media (OM), the most common infection in children and the largest cause of childhood antibiotic prescription. Novel therapeutic strategies that reduce the overall antibiotic consumption due to OM are required because although widespread pneumococcal conjugate immunization has controlled invasive pneumococcal disease, overall OM incidence has not decreased. Biofilm formation represents an important phenotype contributing to the antibiotic tolerance and persistence of S. pneumoniae in chronic or recurrent OM. We investigated the treatment of pneumococcal biofilms with nitric oxide (NO), an endogenous signaling molecule and therapeutic agent that has been demonstrated to trigger biofilm dispersal in other bacterial species. We hypothesised that addition of low concentrations of NO to pneumococcal biofilms would improve antibiotic efficacy and higher concentrations exert direct antibacterial effects. Unlike in many other bacterial species, low concentrations of NO, did not result in S. pneumoniae biofilm dispersal. Instead, treatment of both in vitro biofilms and ex vivo adenoid tissue samples (a reservoir for S. pneumoniae biofilms) with low concentrations of NO enhanced pneumococcal killing when combined with amoxicillin-clavulanic acid, an antibiotic commonly used to treat chronic OM. Quantitative proteomic analysis using iTRAQ (isobaric tag for relative and absolute quantitation) identified 13 proteins that were differentially expressed following low-concentration NO treatment, 85% of which function in metabolism or translation. Treatment with low-concentration NO therefore appears to modulate pneumococcal metabolism and may represent a novel therapeutic approach to reduce antibiotic tolerance in pneumococcal biofilms

Relativistic electron beams accelerated by an interplanetary shock

Collisionless shock waves have long been considered amongst the most prolific particle accelerators in the universe. Shocks alter the plasma they propagate through and often exhibit complex evolution across multiple scales. Interplanetary (IP) traveling shocks have been recorded in-situ for over half a century and act as a natural laboratory for experimentally verifying various aspects of large-scale collisionless shocks. A fundamentally interesting problem in both helio and astrophysics is the acceleration of electrons to relativistic energies (more than 300 keV) by traveling shocks. This letter presents first observations of field-aligned beams of relativistic electrons upstream of an IP shock observed thanks to the instrumental capabilities of Solar Orbiter. This study aims to present the characteristics of the electron beams close to the source and contribute towards understanding their acceleration mechanism. On 25 July 2022, Solar Orbiter encountered an IP shock at 0.98 AU. The shock was associated with an energetic storm particle event which also featured upstream field-aligned relativistic electron beams observed 14 minutes prior to the actual shock crossing. The distance of the beam's origin was investigated using a velocity dispersion analysis (VDA). Peak-intensity energy spectra were anaylzed and compared with those obtained from a semi-analytical fast-Fermi acceleration model. By leveraging Solar Orbiter's high-time resolution Energetic Particle Detector (EPD), we have successfully showcased an IP shock's ability to accelerate relativistic electron beams. Our proposed acceleration mechanism offers an explanation for the observed electron beam and its characteristics, while we also explore the potential contributions of more complex mechanisms.Comment: Main text: 6 pages, 2 figures. Supplementary material: 6 pages, 7 figure

A Pan-Canadian Validation Study for the Detection of EGFR T790M Mutation Using Circulating Tumor DNA From Peripheral Blood

Introduction: Genotyping circulating tumor DNA (ctDNA) is a promising noninvasive clinical tool to identify the EGFR T790M resistance mutation in patients with advanced NSCLC with resistance to EGFR inhibitors. To facilitate standardization and clinical adoption of ctDNA testing across Canada, we developed a 2-phase multicenter study to standardize T790M mutation detection using plasma ctDNA testing. Methods: In phase 1, commercial reference standards were distributed to participating clinical laboratories, to use their existing platforms for mutation detection. Baseline performance characteristics were established using known and blinded engineered plasma samples spiked with predetermined concentrations of T790M, L858R, and exon 19 deletion variants. In phase II, peripheral blood collected from local patients with known EGFR activating mutations and progressing on treatment were assayed for the presence of EGFR variants and concordance with a clinically validated test at the reference laboratory. Results: All laboratories in phase 1 detected the variants at 0.5 % and 5.0 % allele frequencies, with no false positives. In phase 2, the concordance with the reference laboratory for detection of both the primary and resistance mutation was high, with next-generation sequencing and droplet digital polymerase chain reaction exhibiting the best overall concordance. Data also suggested that the ability to detect mutations at clinically relevant limits of detection is generally not platform-specific, but rather impacted by laboratory-specific practices. Conclusions: Discrepancies among sending laboratories using the same assay suggest that laboratory-specific practices may impact performance. In addition, a negative or inconclusive ctDNA test should be followed by tumor testing when possible