Identification of Human Papillomavirus genotypes in juvenile laryngeal papillomatosis. Three-year experience in a concentration hospital in Puebla, Mexico

uvenile laryngeal papillomatosis (JLP) is a chronic benign disease of viral etiology, with an aggressive clinical course. In Mexico, the genotypes of the Human Papilloma Virus (HPV) that cause the disease have been poorly studied. The aim of this study was to identify HPV genotypes in PLJ patients. A descriptive and retrospective study was carried out, the records of patients with PLJ treated in a hospital in Mexico were reviewed, in the period 2018-2021. HPV was identied in all patients for genomes 6, 11, 16, and 18. nine patients were included, 56% women, mean age 9.5 ± 5.7 years; seven patients registered positivity for HPV-11 and 2 for HPV-6. The average age at diagnosis was 2.35 ± 1.77 years, with an average of 12 ± 11.56 surgical procedures. In conclusion, the most frequent genotypes in patients with PLJ were HPV-6 and HPV-11, with the latter predominating

Identificación de genotipos de Virus del Papiloma Humano en papilomatosis laríngea juvenil. Experiencia de 3 años en un hospital de concentración en Puebla, México: Identification of Human Papillomavirus genotypes in juvenile laryngeal papillomatosis. Three-year experience in a concentration hospital in Puebla, Mexico

Background: Juvenile laryngeal papillomatosis (JLP) is a chronic benign disease from viral etiology, whose clinical course can be aggressive. In Mexico, the Human Papillomavirus (HPV) genotypes that cause this disease have been poorly studied. Objective: To identify the HPV genotypes in patients with JLP in a reference Hospital in Puebla, Mexico. Mehods: A retrospective descriptive study was performed in patients with JLP attended in a 3rd level care of the Instituto Mexicano del Seguro Social in Puebla, México, from 2018 to 2021. Medical records were revised. In all patients, HPV identification was performed by polymerase chain reaction for genomes 6, 11, 16 and 18 using specific oligonucleotides. Descriptive statistics were applied. Results: 9 patients were included, 56% women, mean age 9.5 ±5.7 years; 7 patients were HPV-11 positive and 2 HPV-6. The mean age at diagnosis was 2.35 ±1.77 years, with an average of 12 ±11.56 surgical procedures; 5 patients were tracheostomy carriers, 4 had genotype 11; 8 patients were born vaginally, but no patient had maternal genital condylomatous lesions. In the patient born by cesarean section, transmission due to sexual abuse was documented. Conclusions: The most frequent genotypes in patients with JLP in the south-central region of Mexico are HPV-6 and HPV-11, the latter one is predominating.Introducción: La papilomatosis laríngea juvenil (PLJ) es una enfermedad benigna crónica de etiología viral, que tiende a tomar un curso clínico agresivo. En México se han estudiado pobremente los genotipos del Virus del Papiloma Humano (VPH) que causan la enfermedad. Objetivo: Identificar los genotipos del VPH en los pacientes con PLJ en un hospital de concentración en Puebla, México. Métodos: Se realizó un estudio descriptivo y retrospectivo a los pacientes con papilomatosis laríngea juvenil atendidos en un hospital de 3er nivel de atención del Instituto Mexicano del Seguro Social en Puebla, México, en el periodo 2018-2021. Se realizó revisión de expedientes clínicos. En todos los pacientes se identificó el VPH por reacción en cadena de polimerasa para los genomas 6, 11, 16 y 18 utilizando oligonulceótidos específicos. Se aplicó estadística descriptiva. Resultados: Se incluyeron 9 pacientes, 56% mujeres, edad media 9.5 ±5.7 años; 7 pacientes registraron positividad al VPH-11 y 2 al VPH-6. La edad media al diagnóstico fue de 2.35 ±1.77 años, con promedio de procedimientos quirúrgicos de 12 ±11.56; de los 5 pacientes portadores de traqueostomía, 4 fueron positivos a VPH-11; 8 pacientes nacieron por vía vaginal, sin embargo, en ningún caso se reportaron lesiones condilomatosas maternas. En el paciente nacido por cesárea, se documentó transmisión por abuso sexual. Conclusiones: Los genotipos más frecuentes en pacientes con PLJ en la región centro-sur de México son VPH-6 y VPH-11, predominando este último