The Concentration of Zn, Fe, Mn, Cu and Se in Fiber Fractions of Legumes in Indonesia

This study was carried out to evaluate concentration of micro minerals (Zn, Fe, Mn, Cu and Se) of forages and their distribution in fiber fraction (neutral detergent fiber/NDF and acid detergent fiber/ADF) in West Sumatra during dry and rainy seasons. Four species of common legume namely Leucaena leucocephala, Centrocema pubescens, Calopogonium mucunoides and Acacia mangium were collected at native pasture during rainy and dry seasons. The results showed that micro minerals concentration of forages and their distribution in fiber fraction varied among species and season. In general, concentration of micro minerals was slightly higher in rainy season compared to dry season either in legumes forages. Data on legume forages showed that 75% of legumes were deficient in Zn and Mn, 62.5 % deficient in Cu and 50 % deficient in Se. There was no species of legume deficient in Fe. Distribution of micro minerals in NDF and ADF were also significantly affected by species and season and depends on the kinds of element measured. Generally, micro minerals were associated in fiber fractions and it yield much higher during dry season compared to rainy season. Iron (Fe) and selenium (Se) in forages were the highest elements associated in NDF and ADF, while the lowest was found in Copper (Cu). (Animal Production 12(2): 105-110 (2010

Additional file 1: Figure S1. of Angiogenic potency evaluation of cell therapy candidates by a novel application of the in vitro aortic ring assay

Showing cellular fractions of aortic ring cultures isolated and processed for flow cytometry analysis. Fluorophore-conjugated antibody against human specific cell surface marker (TRA-1-85(APC)) was applied in combination with MSC/pericyte marker (CD146(FITC), A) or endothelial marker (CD31(FITC), B) specific antibodies. Cell population positive for TRA-1-85 (y axis) tested positive for MSC/pericyte marker (CD146 (A, Q6)) and slight positivity for endothelial marker (CD31 (B, Q1)). This suggests that FTM HUCPVCs maintained their perivascular cell properties in aortic ring cocultures and did not develop an endothelial phenotype. Quadrants defined using isotype controls matching applied primary antibodies. (PDF 91 kb

Additional file 3: of In vitro generation of Sertoli-like and haploid spermatid-like cells from human umbilical cord perivascular cells

Supplementary Figure S1–S4 (PPTX 5065 kb