In vitro susceptibility characteristics of Cryptococcus neoformans varieties from AIDS patients in Goiânia, Brazil

Sixty clinical isolates of Cryptococcus neoformans from AIDS from Goiânia, state of Goiás, Brazil, were characterized according to varieties, serotypes and tested for antifungal susceptibility. To differentiate the two varieties was used L-canavanine-glycine-bromothymol blue medium and to separate the serotypes was used slide agglutination test with Crypto Check Iatron. The Minimal Inhibitory Concentration (MIC) of fluconazole, itraconazole, and amphotericin B were determined by the National Committee for Clinical Laboratory Standards macrodilution method. Our results identified 56 isolates as C. neoformans var. neoformans serotype A and 4 isolates as C. neoformans var. gattii serotype B. MIC values for C. neoformans var. gattii were higher than C. neoformans var. neoformans. We verified that none isolate was resistant to itraconazole and to amphotericin B, but one C. neoformans var. neoformans and three C. neoformans var. gattii isolates were resistant to fluconazole. The presence of C. neoformans var. gattii fluconazole resistant indicates the importance of determining not only the variety of C. neoformans infecting the patients but also measuring the MIC of the isolate in order to properly orient treatment

miR-CLIP capture of a miRNA targetome uncovers a lincRNA H19-miR-106a interaction

Identifying the interaction partners of noncoding RNAs is essential for elucidating their functions. We have developed an approach, termed microRNA crosslinking and immunoprecipitation (miR-CLIP), using pre-miRNAs modified with psoralen and biotin to capture their targets in cells. Photo-crosslinking and Argonaute 2 immunopurification followed by streptavidin affinity purification of probe-linked RNAs provided selectivity in the capture of targets, which were identified by deep sequencing. miR-CLIP with pre-miR-106a, a miR-17-5p family member, identified hundreds of putative targets in HeLa cells, many carrying conserved sequences complementary to the miRNA seed but also many that were not predicted computationally. miR-106a overexpression experiments confirmed that miR-CLIP captured functional targets, including H19, a long noncoding RNA that is expressed during skeletal muscle cell differentiation. We showed that miR-17-5p family members bind H19 in HeLa cells and myoblasts. During myoblast differentiation, levels of H19, miR-17-5p family members and mRNA targets changed in a manner suggesting that H19 acts as a 'sponge' for these miRNAs

All-sky measurement of the anisotropy of cosmic rays at 10 TeV and mapping of the local interstellar magnetic field

We present the first full-sky analysis of the cosmic ray arrival direction distribution with data collected by the High-Altitude Water Cherenkov and IceCube observatories in the northern and southern hemispheres at the same median primary particle energy of 10 TeV. The combined sky map and angular power spectrum largely eliminate biases that result from partial sky coverage and present a key to probe into the propagation properties of TeV cosmic rays through our local interstellar medium and the interaction between the interstellar and heliospheric magnetic fields. From the map, we determine the horizontal dipole components of the anisotropy δ 0h = 9.16 × 10−4 and δ 6h = 7.25 × 10−4 (±0.04 × 10−4). In addition, we infer the direction (229fdg2 ± 3fdg5 R.A., 11fdg4 ± 3fdg0 decl.) of the interstellar magnetic field from the boundary between large-scale excess and deficit regions from which we estimate the missing corresponding vertical dipole component of the large-scale anisotropy to be ${\delta }_{N}\sim -{3.97}_{-2.0}^{+1.0}\times {10}^{-4}$