DESENVOLVIMENTO DE FILMES POLIMÉRICOS NANOESTRUTURADOS DE poli[Ni(α,α’-Me2Salen)]–OH COMO SENSOR BIOMIMÉTICO NA DETECÇÃO DE GLICEROL

Um sensor biomimético baseado no eletrodo de platina eletroquimicamente modificado com filme polimérico nanoestruturado de poli[Ni(α,α’-Me2Salen)]–OH para detecção e quantificação de glicerol foi desenvolvido. A eletropolimerização, ativação, comportamento eletroquímico do sensor e a eletrooxidação de glicerol foram investigados utilizando a técnica de voltametria cíclica. A resposta voltamétrica do sensor foi obtida em um intervalo de concentração de 4,99 × 10-7 mol L-1 a 4,76 × 10-5 mol L-1 sendo a resposta linear entre 4,99 x 10-7 mol L-1 e 3,73 x 10-6 mol L-1 com limite de quantificação de 38,4 µmol L-1, limite de detecção de 3,82 µmol L-1 e sensibilidade de 1,82 μA L mol-1. O sensor apresentou característica cinética semelhante ao modelo de Michaelis-Menten, sendo então os parâmetros cinéticos calculados por Lineweaver-Burke e pelo “ajuste de curva não linear”

AZO-POLÍMERO MOLECULARMENTE IMPRESSO COMO ESTRATÉGIA NO DESENVOLVIMENTO DE UMA NOVA PLATAFORMA SENSORIAL PARA DETECÇÃO DE ÁCIDO ÚRICO

This present work was studied the development of a molecularly imprinted polymer (MIP) based on the polyazo-Bismarck Y) for detection of uric acid. The film MIP was electrodeposited on the fluorine-doped tin-oxide (FTO) glass electrode surface by electropolymerization of Bismark Brown Y monomer in presence of uric acid as the template molecule. The MIP-poly(azo-Bismark Y) sensor displays a good electrochemical response, high sensitivity and selectivity in specific binding of the uric acid. Under the optimized experimental conditions, the voltammetric response of the proposed sensor was linear in the concentration range of 0.20 mmol L-1 to 0.60 mmol L-1, with detection limit of 0.17 mmol L−1.No presente trabalho foi estudado o desenvolvimento de um polímero molecularmente impresso (PMI) baseado no poli(azo-Bismarck Y) para detecção de ácido úrico. O filme PMI foi preparado pela eletropolimerização do monômero Bismarck Brown Y na presença de ácido úrico como molécula molde sobre a superfície do eletrodo de óxido de estanho dopado com flúor  (FTO). O sensor PMI-poli(azo-Bismark Y) exibiu boa resposta eletroquímica, alta sensibilidade e seletividade na ligação específica ao ácido úrico. Sob as condições experimentais otimizadas, a resposta voltamétrica do sensor proposto foi linear no intervalo de concentração de 0,20 mmol L-1 a 0,60 mmol L-1, com limite de detecção de 0,17 mmol L−1

ESTUDO DAS PROPRIEDADES FOTOELETROQUÍMICAS DO POLI(AZO-BISMARCK BROWN R) OBTIDO POR ELETROPOLIMERIZAÇÃO PARA SENSIBILIZAÇÃO DE CÉLULAS SOLARES

the present research investigates the electrochemical behavior of a solar cell sensitized with Bismark Brown R azopolymer. This cell was developed from the electropolymerization of the dye on a conductive glass substrate coated with fluorine-doped tin oxide (FTO) by cyclic voltammetry. The photosensitive behavior of the obtained film was analyzed by cyclic voltammetry and chronoamperometry in the presence of pulsed light. The poly(azo-Bismarck R) exhibited reversible light-induced changes of electrical conductance, due to azo group photoisomerization. Electrochemical impedance spectroscopy was also carried out under a light source. This technique allowed to evaluate interfacial phenomena existing in the photovoltaic device, performing a detailed investigation of its photoelectrochemical properties. A decrease in eletrical resistance was observed when light was induced on the polymer membrane indicating charge transfer to the polymer conduction band. Thus, photo-responsive film of Bismarck Brown can be developed for advanced organic photovoltaic such as solar cells.O presente trabalho investiga o comportamento eletroquímico de uma célula solar sensibilizada com azopolímero Bismark Brown R. Esta célula foi desenvolvida a partir da eletropolimerização do corante sobre um substrato de vidro condutor revestido com óxido de estanho dopado com flúor (FTO) por voltametria cíclica. O comportamento fotossensível do filme obtido foi analisado por voltametria cíclica e cronoamperometria na presença de luz pulsada. O poli (azo-Bismarck R) exibiu mudanças reversíveis de condutividade elétrica induzidas pela luz, devido à fotoisomerização do grupo azo. A espectroscopia de impedância eletroquímica também foi realizada sob uma fonte de luz. Esta técnica permitiu avaliar os fenômenos interfaciais existentes no dispositivo fotovoltaico, realizando uma investigação detalhada de suas propriedades fotoeletroquímicas. Uma diminuição da resistência foi observado quando foi indicido a luz sobre a membrana polimérica indicando a transferência de carga para a banda de condução do polímero. Assim, o filme foto-sensível de Bismarck Brown R, apresenta potencial aplicação para dispositivos fotovoltaicos orgânicos avançados, tais como células solares

Nanocomposite Materials Based on Electrochemically Synthesized Graphene Polymers: Molecular Architecture Strategies for Sensor Applications

The use of graphene and its derivatives in the development of electrochemical sensors has been growing in recent decades. Part of this success is due to the excellent characteristics of such materials, such as good electrical and mechanical properties and a large specific surface area. The formation of composites and nanocomposites with these two materials leads to better sensing performance compared to pure graphene and conductive polymers. The increased large specific surface area of the nanocomposites and the synergistic effect between graphene and conducting polymers is responsible for this interesting result. The most widely used methodologies for the synthesis of these materials are still based on chemical routes. However, electrochemical routes have emerged and are gaining space, affording advantages such as low cost and the promising possibility of modulation of the structural characteristics of composites. As a result, application in sensor devices can lead to increased sensitivity and decreased analysis cost. Thus, this review presents the main aspects for the construction of nanomaterials based on graphene oxide and conducting polymers, as well as the recent efforts made to apply this methodology in the development of sensors and biosensors

Trupe quimiatividade: desenvolvimento de abordagens e experimentos de forma lúdica para a difusão da ciência química

In order to demystify science and chemistry approach the students of this science as this on a daily basis, the Project Extension Show chemistry developed by Troupe Quimiatividade group develops dissemination activities of science through presentations of demonstration experiments with a playful approach and interactive.Com o intuito de desmistificar a Ciência Química e aproximar os alunos desta ciência tão presente no dia a dia, o Projeto de Extensão Show da Química desenvolvido pelo grupo Trupe Quimiatividade desenvolve atividades de difusão da ciência através de apresentações de experimentos demonstrativos com uma abordagem lúdica e interativa

Educação ambiental e química através de uma oficina de reciclagem de óleo de cozinha: aprendendo a fazer sabão

We need to educate adults and children about the incorrect disposal of used cooking oil, and about the damage that can be caused to the environment. Information, alone, is not enough if we do not suggest alternatives, for example, recycling of the oil to manufacture soap. In this work, werelate chemical knowledge with everyday experience, throughout workshops offered in schools, in which not only theoretical information is provided, but also a practical part when the students make soap through the saponification reaction of the oil.É necessário alertar adultos e crianças sobre o descarte incorreto do óleo de cozinha usado e sobre os danos que podem ser causados ao meio ambiente por essa prática. Somente a informação não será suficiente se não apontarmos alternativas, como por exemplo, a reciclagem do óleo para a fabricação de sabão. Neste trabalho, procuramos relacionarosconhecimentos químicos com o cotidiano, através de oficinas aplicadas em escolas que além de informações teóricas também conta com uma parte prática em que através de uma reação de saponificação o óleo é transformado em sabão

Mild to moderate post-COVID-19 alters markers of lymphocyte activation, exhaustion, and immunometabolic responses that can be partially associated by physical activity level— an observational sub-analysis fit- COVID study

AimThis study aimed to evaluate if physical activity is associated with systemic and cellular immunometabolic responses, in young adults after mild-to-moderate COVID-19 infection.MethodsMild- to- moderate post-COVID-19 patients (70.50 ± 43.10 days of diagnosis; age: 29.4 (21.9– 34.9) years; BMI: 25.5 ± 4.3 kg m2 n = 20) and healthy age-matched controls (age: 29.3 (21.2 – 32.6) years; BMI: 25.4 ± 4.7 kg m2; n = 20) were evaluated. Physical activity levels (PAL), body composition, dietary habits, muscular and pulmonary function, mental health, sleep quality, metabolic parameters, immune phenotypic characterization, stimulated whole blood and PBMC culture (cytokine production), mRNA, and mitochondrial respiration in PBMCs were evaluated. ResultsThe post-COVID-19 group exhibited lower levels of moderate to vigorous physical activity (MVPA) (p = 0.038); therefore, all study comparisons were performed with adjustment for MVPA. Post-COVID-19 impacted the pulmonary function (FEV1, FEV1%pred, FVC, and FVC %pred) compared with the control (p adjusted by MVPA (p adj) <0.05). Post-COVID-19 exhibited lower levels of serum IL-6 (p adj <0.01), whereas it showed higher serum IL-10, triglyceride, leptin, IgG, ACE activity, TNFRSF1A, and PGE2 (p adj <0.05) levels compared with controls. Post-COVID-19 presented a lower percentage of Treg cells (p adj = 0.03) and altered markers of lymphocyte activation and exhaustion (lower CD28 expression in CD8+ T cells (p adj = 0.014), whereas CD4+T cells showed higher PD1 expression (p adj = 0.037)) compared with the control group. Finally, post- COVID-19 presented an increased LPS-stimulated whole- blood IL-10 concentration (p adj <0.01). When exploring mitochondrial respiration and gene expression in PBMCs, we observed a higher LEAK state value (p adj <0.01), lower OXPHOS activity (complex I) (p adj = 0.04), and expression of the Rev-Erb-α clock mRNA after LPS stimulation in the post-COVID-19 patients than in the control (p adj <0.01). Mainly, PAL was associated with changes in IL-10, triglyceride, and leptin levels in the plasma of post-COVID-19 patients. PAL was also associated with modulation of the peripheral frequency of Treg cells and the expression of PD-1 in CD8+ T cells, although it abrogated the statistical effect in the analysis of TNF-α and IL-6 production by LPS- and PMA-stimulated PBMC of post-COVID-19 patients. ConclusionYoung adults after mild-to-moderate SARS-CoV-2 infection appeared to have lower physical activity levels, which can be associated with clinical and immunometabolic responses in a complex manner

Image_2_Mild to moderate post-COVID-19 alters markers of lymphocyte activation, exhaustion, and immunometabolic responses that can be partially associated by physical activity level— an observational sub-analysis fit- COVID study.tif

AimThis study aimed to evaluate if physical activity is associated with systemic and cellular immunometabolic responses, in young adults after mild-to-moderate COVID-19 infection.MethodsMild- to- moderate post-COVID-19 patients (70.50 ± 43.10 days of diagnosis; age: 29.4 (21.9– 34.9) years; BMI: 25.5 ± 4.3 kg m2 n = 20) and healthy age-matched controls (age: 29.3 (21.2 – 32.6) years; BMI: 25.4 ± 4.7 kg m2; n = 20) were evaluated. Physical activity levels (PAL), body composition, dietary habits, muscular and pulmonary function, mental health, sleep quality, metabolic parameters, immune phenotypic characterization, stimulated whole blood and PBMC culture (cytokine production), mRNA, and mitochondrial respiration in PBMCs were evaluated. ResultsThe post-COVID-19 group exhibited lower levels of moderate to vigorous physical activity (MVPA) (p = 0.038); therefore, all study comparisons were performed with adjustment for MVPA. Post-COVID-19 impacted the pulmonary function (FEV1, FEV1%pred, FVC, and FVC %pred) compared with the control (p adjusted by MVPA (p adj) 2 (p adj + T cells (p adj = 0.014), whereas CD4+T cells showed higher PD1 expression (p adj = 0.037)) compared with the control group. Finally, post- COVID-19 presented an increased LPS-stimulated whole- blood IL-10 concentration (p adj ConclusionYoung adults after mild-to-moderate SARS-CoV-2 infection appeared to have lower physical activity levels, which can be associated with clinical and immunometabolic responses in a complex manner.</p

Image_3_Mild to moderate post-COVID-19 alters markers of lymphocyte activation, exhaustion, and immunometabolic responses that can be partially associated by physical activity level— an observational sub-analysis fit- COVID study.tif

AimThis study aimed to evaluate if physical activity is associated with systemic and cellular immunometabolic responses, in young adults after mild-to-moderate COVID-19 infection.MethodsMild- to- moderate post-COVID-19 patients (70.50 ± 43.10 days of diagnosis; age: 29.4 (21.9– 34.9) years; BMI: 25.5 ± 4.3 kg m2 n = 20) and healthy age-matched controls (age: 29.3 (21.2 – 32.6) years; BMI: 25.4 ± 4.7 kg m2; n = 20) were evaluated. Physical activity levels (PAL), body composition, dietary habits, muscular and pulmonary function, mental health, sleep quality, metabolic parameters, immune phenotypic characterization, stimulated whole blood and PBMC culture (cytokine production), mRNA, and mitochondrial respiration in PBMCs were evaluated. ResultsThe post-COVID-19 group exhibited lower levels of moderate to vigorous physical activity (MVPA) (p = 0.038); therefore, all study comparisons were performed with adjustment for MVPA. Post-COVID-19 impacted the pulmonary function (FEV1, FEV1%pred, FVC, and FVC %pred) compared with the control (p adjusted by MVPA (p adj) 2 (p adj + T cells (p adj = 0.014), whereas CD4+T cells showed higher PD1 expression (p adj = 0.037)) compared with the control group. Finally, post- COVID-19 presented an increased LPS-stimulated whole- blood IL-10 concentration (p adj ConclusionYoung adults after mild-to-moderate SARS-CoV-2 infection appeared to have lower physical activity levels, which can be associated with clinical and immunometabolic responses in a complex manner.</p

Table_2_Mild to moderate post-COVID-19 alters markers of lymphocyte activation, exhaustion, and immunometabolic responses that can be partially associated by physical activity level— an observational sub-analysis fit- COVID study.docx

AimThis study aimed to evaluate if physical activity is associated with systemic and cellular immunometabolic responses, in young adults after mild-to-moderate COVID-19 infection.MethodsMild- to- moderate post-COVID-19 patients (70.50 ± 43.10 days of diagnosis; age: 29.4 (21.9– 34.9) years; BMI: 25.5 ± 4.3 kg m2 n = 20) and healthy age-matched controls (age: 29.3 (21.2 – 32.6) years; BMI: 25.4 ± 4.7 kg m2; n = 20) were evaluated. Physical activity levels (PAL), body composition, dietary habits, muscular and pulmonary function, mental health, sleep quality, metabolic parameters, immune phenotypic characterization, stimulated whole blood and PBMC culture (cytokine production), mRNA, and mitochondrial respiration in PBMCs were evaluated. ResultsThe post-COVID-19 group exhibited lower levels of moderate to vigorous physical activity (MVPA) (p = 0.038); therefore, all study comparisons were performed with adjustment for MVPA. Post-COVID-19 impacted the pulmonary function (FEV1, FEV1%pred, FVC, and FVC %pred) compared with the control (p adjusted by MVPA (p adj) 2 (p adj + T cells (p adj = 0.014), whereas CD4+T cells showed higher PD1 expression (p adj = 0.037)) compared with the control group. Finally, post- COVID-19 presented an increased LPS-stimulated whole- blood IL-10 concentration (p adj ConclusionYoung adults after mild-to-moderate SARS-CoV-2 infection appeared to have lower physical activity levels, which can be associated with clinical and immunometabolic responses in a complex manner.</p