GENDERED LIVELIHOOD IMPLICATIONS FOR IMPROVEMENTS OF LIVESTOCK WATER PRODUCTIVITY IN ZIMBABWE

Scarcity, lack of access, and ineffective and inefficient use of water in Nkayi District, Zimbabwe, threaten agricultural production. The purpose of this study is to augment understanding of opportunities to increase livestock water productivity (LWP) in Nkayi District by taking into account key differences in the capacities, opportunities, and needs of women and men. There are two important types of female-headed households, de facto and de jure. The results from this study showed thatmale-headed and de facto and de jure female-headed households sharemuch in common.They all had similar areas of cropland and access to education, finances, veterinary and extension services, and transportation and markets. Households of all types had similar herd sizes. All were desperately poor with incomes much less than a dollar a day. To rise out of poverty, the knowledge, skills and effort of all household heads will be needed. In spite of severe poverty, household heads of all types are literate and have sufficient education that can help enable adoption of intervention options that can lead to increased agricultural production and improved livelihoods. The results also showed that major differences exist in terms of the roles of men and women in ownership, management and decision making related to livestock keeping and animal production. Men clearly dominate in both ownership and decision making even though women play a major role in animal management. Only in de jure female-headed households were womenmore likely thanmen to own cattle and goats. They were also more likely to be involved in farming as a primary livelihood activity. Surprisingly, men were more likely to be involved in animal management in these de jure female-headed households. Women were also excluded from water users’ and livestock producers’ associations although a minority of men was members. By not involving the already-developed capacity of women, the community loses out on a significant opportunity to increase LWP and animal production more widely. Greater inclusion of women in decision making will be an important part of future efforts to improve livelihoods through livestock development

A Sensing Role of the Glutamine Synthetase in the Nitrogen Regulation Network in Fusarium fujikuroi

Contains fulltext : 125173.pdf (publisher's version ) (Open Access)In the plant pathogenic ascomycete Fusarium fujikuroi the synthesis of several economically important secondary metabolites (SM) depends on the nitrogen status of the cells. Of these SMs, gibberellin and bikaverin synthesis is subject to nitrogen catabolite repression (NCR) and is therefore only executed under nitrogen starvation conditions. How the signal of available nitrogen quantity and quality is sensed and transmitted to transcription factors is largely unknown. Earlier work revealed an essential regulatory role of the glutamine synthetase (GS) in the nitrogen regulation network and secondary metabolism as its deletion resulted in total loss of SM gene expression. Here we present extensive gene regulation studies of the wild type, the Deltagln1 mutant and complementation strains of the gln1 deletion mutant expressing heterologous GS-encoding genes of prokaryotic and eukaryotic origin or 14 different F. fujikuroi gln1 copies with site-directed mutations. All strains were grown under different nitrogen conditions and characterized regarding growth, expression of NCR-responsive genes and biosynthesis of SM. We provide evidence for distinct roles of the GS in sensing and transducing the signals to NCR-responsive genes. Three site directed mutations partially restored secondary metabolism and GS-dependent gene expression, but not glutamine formation, demonstrating for the first time that the catalytic and regulatory roles of GS can be separated. The distinct mutant phenotypes show that the GS (1) participates in NH4 (+)-sensing and transducing the signal towards NCR-responsive transcription factors and their subsequent target genes; (2) affects carbon catabolism and (3) activates the expression of a distinct set of non-NCR GS-dependent genes. These novel insights into the regulatory role of the GS provide fascinating perspectives for elucidating regulatory roles of GS proteins of different organism in general

Microbiomics of Namib Desert habitats

The Namib Desert is one of the world’s only truly coastal desert ecosystem. Until the end of the 1st decade of the twenty-first century, very little was known of the microbiology of this southwestern African desert, with the few reported studies being based solely on culture-dependent approaches. However, from 2010, an intense research program was undertaken by researchers from the University of the Western Cape Institute for Microbial Biotechnology and Metagenomics, and subsequently the University of Pretoria Centre for Microbial Ecology and Genomics, and their collaborators, led to a more detailed understanding of the ecology of the indigenous microbial communities in many Namib Desert biotopes. Namib Desert soils and the associated specialized niche communities are inhabited by a wide array of prokaryotic, lower eukaryotic and virus/phage taxa. These communities are highly heterogeneous on both small and large spatial scales, with community composition impacted by a range of macro- and micro-environmental factors, from water regime to soil particle size. Community functionality is also surprisingly non-homogeneous, with some taxa retaining functionality even under hyper-arid soil conditions, and with subtle changes in gene expression and phylotype abundances even on diel timescales. Despite the growing understanding of the structure and function of Namib Desert microbiomes, there remain enormous gaps in our knowledge. We have yet to quantify many of the processes in these soil communities, from regional nutrient cycling to community growth rates. Despite the progress that has been made, we still have little knowledge of either the role of phages in microbial community dynamics or inter-species interactions. Furthermore, the intense research efforts of the past decade have highlighted the immense scope for future microbiological research in this dynamic, enigmatic and charismatic region of Afric