Antigen-driven T-cell turnover.

A mathematical model is developed to characterize the distribution of cell turnover rates within a population of T lymphocytes. Previous models of T-cell dynamics have assumed a constant uniform turnover rate; here we consider turnover in a cell pool subject to clonal proliferation in response to diverse and repeated antigenic stimulation. A basic framework is defined for T-cell proliferation in response to antigen, which explicitly describes the cell cycle during antigenic stimulation and subsequent cell division. The distribution of T-cell turnover rates is then calculated based on the history of random exposures to antigens. This distribution is found to be bimodal, with peaks in cell frequencies in the slow turnover (quiescent) and rapid turnover (activated) states. This distribution can be used to calculate the overall turnover for the cell pool, as well as individual contributions to turnover from quiescent and activated cells. The impact of heterogeneous turnover on the dynamics of CD4(+) T-cell infection by HIV is explored. We show that our model can resolve the paradox of high levels of viral replication occurring while only a small fraction of cells are infected

Mortality in patients with successful initial response to highly active antiretroviral therapy is still higher than in non-HIV-infected individuals.

Mortality in HIV-infected patients has decreased dramatically since the introduction of highly active antiretroviral therapy (HAART). We analyzed progression to death in a population of 3678 antiretroviral treatment-naive patients from the ATHENA national observational cohort from 24 weeks after the start of HAART. Mortality was compared with that in the general population in the Netherlands matched by age and gender. Only log-transformed CD4 cell count (hazard ratio [HR] = 0.50, 95% confidence interval [CI]: 0.40 to 0.61 per unit increase) and plasma viral load (HR = 0.30, 95% CI: 0.15 to 0.60, HIV RNA level or = 100,000 copies/mL) measured at 24 weeks and infection via intravenous drug use (IDU) (HR = 0.16, 95% CI: 0.10 to 0.26, non-IDU vs. IDU) were significantly associated with progression to death. For non-IDU patients with 600 x 10 CD4 cells/L and an HIV RNA level <100,000 copies/mL at 24 weeks, mortality was predicted to be 5.3 (95% CI: 3.5 to 8.4) and 10.4 (95% CI: 6.4 to 17.4) times higher than in the general population for 25-year-old men and women, respectively, and 1.15 (95% CI: 1.08 to 1.25) and 1.29 (95% CI: 1.16 to 1.50) times higher for 65-year-old men and women, respectively. Hence, mortality in HIV-infected patients with a good initial response to HAART is still higher than in the general population

Artificial biofilm thickness and salivary flow effects on fluoride efficacy – A model development study

This laboratory model development study investigated the interaction between artificial biofilm thickness and salivary flow rate on fluoride-mediated prevention of enamel caries lesion formation. This 5-day pH cycling study on sound bovine enamel specimens utilized a continuous flow model and followed a 4 (agarose biofilm thickness-‘no biofilm’/1/2/3mm)×2 (remineralizing solution flow rate-0.05/0.5ml/min)×2 (fluoride-0/383ppm as sodium fluoride) factorial design. Vickers surface microhardness change was the outcome measure. Data were analyzed with three-way ANOVA. The three-way interaction gel thickness×flow rate×fluoride concentration was significant (p=0.0006). 383ppm fluoride caused less softening than 0ppm regardless of gel thickness or flow rate. 0.5ml/min flow rate caused less softening than 0.05ml/min for ‘no biofilm’ and 1mm biofilm thickness regardless of fluoride concentration, for 2 and 3mm with 0ppm F but not for 383ppm F. For 0.05ml/min, softening was reduced as gel thickness increased from ‘no biofilm’-1-2mm, but not from 2-3mm. For 0.5ml/min, ‘no biofilm’ caused more softening than 1, 2, and 3mm, but 1, 2, and 3mm were not different from each other for both 0 and 383ppm F. The present findings suggest that the efficacy of fluoride in preventing enamel demineralization is affected by both biofilm thickness and salivary flow rate, with both thicker biofilms and higher flow rate resulting in less demineralization

Perturbed disks get shocked. Binary black hole merger effects on accretion disks

The merger process of a binary black hole system can have a strong impact on a circumbinary disk. In the present work we study the effect of both central mass reduction (due to the energy loss through gravitational waves) and a possible black hole recoil (due to asymmetric emission of gravitational radiation). For the mass reduction case and recoil directed along the disk's angular momentum, oscillations are induced in the disk which then modulate the internal energy and bremsstrahlung luminosities. On the other hand, when the recoil direction has a component orthogonal to the disk's angular momentum, the disk's dynamics are strongly impacted, giving rise to relativistic shocks. The shock heating leaves its signature in our proxies for radiation, the total internal energy and bremsstrahlung luminosity. Interestingly, for cases where the kick velocity is below the smallest orbital velocity in the disk (a likely scenario in real AGN), we observe a common, characteristic pattern in the internal energy of the disk. Variations in kick velocity simply provide a phase offset in the characteristic pattern implying that observations of such a signature could yield a measure of the kick velocity through electromagnetic signals alone.Comment: 10 pages, 13 figures. v2: Minor changes, version to be published in PR

Achievement and avoidance games for generating abelian groups

For any finite group G , the DO GENERATE game is played by two players Alpha and Beta as follows. Alpha moves first and chooses x 1 ∈ G . The k -th play consists of a choice of x k ∈ G − S k −1 where S n = {itx 1 ,..., x n }. Let G n = 〈 S n 〉. The game ends when G n = G . The player who moves x n wins. In the corresponding avoidance game, DON'T GENERATE, the last player to move loses. Of course neither game can end in a draw. For an arbitrary group, it is an unsolved problem to determine whether Alpha or Beta wins either game. However these two questions are answered here for abelian groups.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/45834/1/182_2005_Article_BF01756028.pd

Computer simulation of a biomanagement system : the Mendocino County deer population in California

Management of deer populations is directed toward multiple objectives. Deer populations on public and private lands belong to the public and thus management is a political process. Four components for an effective management system for deer populations are identified. These are the set of objectives relating to the resource, the set of regulations which will achieve the objectives, knowledge of the expected population response to alternative management strategies, and a means of monitoring these responses to determine whether or not the objectives are being achieved. Deer provide benefits mainly through the associated recreational opportunities and cause costs by interacting with land based economic activities such as agricultural crop production and reforestation. At certain times of the year deer may also compete with domestic livestock for forage. Deer also cause significant costs through collisions with automobiles on the highways. The extent of these benefits and costs, and others, is related to the biosystem through parameters such as the size and composition of the population, the extent of the hunting kill, and so on. In this thesis a computer simulation model of the Mendocino County, California, deer population is presented. The population is modeled as a density dependent birth and death process. Hunting strategies are potentially the most flexible management tool. Thus the model is structured to permit detailed examination of the response over time of the population to alternative hunting strategies. In California, a bucks-only hunting strategy has been followed since about the turn of the century. This study demonstrates that the bucks-only strategy neither effectively controls the size of the deer population, nor does it provide for the greatest recreational opportunities. The extent of the costs referred to above are directly related to the size of the population and the consumptive recreational benefits, that is those due to hunting, are directly related to the size of the hunting kill. Experiments with the model show that population control can be achieved and the hunting kill can be increased by a mixed buck and antlerless deer hunting strategy. Other results show that the computer simulation model can provide information about the biosystem which is not otherwise available. Simulation methods permit considerable insights into the operation and control of complex biosystems where the status of the systems is time dependent and the systems are influenced by uncontrollable elements so that at best the outcomes resulting from particular management actions are uncertain. The simulation model used in this study is applicable to other deer populations and other wildlife species

A k-shell decomposition method for weighted networks

We present a generalized method for calculating the k-shell structure of weighted networks. The method takes into account both the weight and the degree of a network, in such a way that in the absence of weights we resume the shell structure obtained by the classic k-shell decomposition. In the presence of weights, we show that the method is able to partition the network in a more refined way, without the need of any arbitrary threshold on the weight values. Furthermore, by simulating spreading processes using the susceptible-infectious-recovered model in four different weighted real-world networks, we show that the weighted k-shell decomposition method ranks the nodes more accurately, by placing nodes with higher spreading potential into shells closer to the core. In addition, we demonstrate our new method on a real economic network and show that the core calculated using the weighted k-shell method is more meaningful from an economic perspective when compared with the unweighted one.Comment: 17 pages, 6 figure

A simultaneous search for prompt radio emission associated with the short GRB 170112A using the all-sky imaging capability of the OVRO-LWA

We have conducted the most sensitive low frequency (below 100 MHz) search to date for prompt, low-frequency radio emission associated with short-duration gamma-ray bursts (GRBs), using the Owens Valley Radio Observatory Long Wavelength Array (OVRO-LWA). The OVRO-LWA's nearly full-hemisphere field-of-view ($\sim20$,$000$ square degrees) allows us to search for low-frequency (sub-$100$ MHz) counterparts for a large sample of the subset of GRB events for which prompt radio emission has been predicted. Following the detection of short GRB 170112A by Swift, we used all-sky OVRO-LWA images spanning one hour prior to and two hours following the GRB event to search for a transient source coincident with the position of GRB 170112A. We detect no transient source, with our most constraining $1\sigma$ flux density limit of $650~\text{mJy}$ for frequencies spanning $27~\text{MHz}-84~\text{MHz}$. We place constraints on a number of models predicting prompt, low-frequency radio emission accompanying short GRBs and their potential binary neutron star merger progenitors, and place an upper limit of $L_\text{radio}/L_\gamma \lesssim 7\times10^{-16}$ on the fraction of energy released in the prompt radio emission. These observations serve as a pilot effort for a program targeting a wider sample of both short and long GRBs with the OVRO-LWA, including bursts with confirmed redshift measurements which are critical to placing the most constraining limits on prompt radio emission models, as well as a program for the follow-up of gravitational wave compact binary coalescence events detected by advanced LIGO and Virgo.Comment: 14 pages, 5 figures, ApJ submitte

Anticaries Potential of a Sodium Monofluorophosphate Dentifrice Containing Calcium Sodium Phosphosilicate: Exploratory in situ Randomized Trial

Calcium sodium phosphosilicate (CSPS) is a bioactive glass material that alleviates dentin hypersensitivity and is postulated to confer remineralization of caries lesions. This single-centre, randomized, single (investigator)-blind, placebo-controlled, crossover, in situ study explored whether the addition of 5% CSPS to a nonaqueous fluoride (F) such as sodium monofluorophosphate (SMFP)-containing dentifrice affects its cariostatic ability. Seventy-seven subjects wore 4 gauze-covered enamel specimens with preformed lesions (2 surface-softened and 2 subsurface) placed buccally on their mandibular bilateral dentures for up to 4 weeks. Subjects brushed twice daily with 1 of the 5 study dentifrices: 927 ppm F/5% CSPS, 927 ppm F/0% CSPS, 250 ppm F/0% CSPS, 0 ppm F/5% CSPS, or 0 ppm F/0% CSPS. Specimens were retrieved after either 21 (surface-softened lesions; analyzed by Knoop surface microhardness [SMH]) or 28 days (subsurface lesions; analyzed by transverse microradiography). The enamel fluoride uptake was determined for all specimens using a microbiopsy technique. The concentrations of fluoride and calcium in gauze-retrieved plaque were also evaluated. Higher dentifrice fluoride concentrations led to greater remineralization and fluoridation of both lesion types and increased plaque fluoride concentrations. CSPS did not improve the cariostatic properties of SMFP; there were no statistically significant differences between 927 ppm F/5% CSPS and 927 ppm F/0% CSPS in percent SMH recovery (p = 0.6788), change in integrated mineral loss (p = 0.5908), or lesion depth (p = 0.6622). Likewise, 0 ppm F/5% CSPS did not provide any benefits in comparison to 0 ppm F/0% CSPS. In conclusion, CSPS does not negatively impact nor does it improve the ability of an SMFP dentifrice to affect remineralization of caries lesions

Impediments to Effective Interactions Between Multipurpose Water Districts and Other Governmental Institutions in Urbanizing Areas

Water institutions are highly diffused throughout society. These institutions interact with one another in various ways. As water needs and services expand, collaborative and cooperative arrangements are commonly sought as a means of meeting common goals of providing a safe, dependable adn least cost water supply to particular constituencies. Of the many different institutions involved in the development, managment, distribution, and use of water, perhaps the most significant in terms of extensive interactions with other institutions is the kind that is typified by Water Conservancy Districts and Metropolitan Water Districts in Utah. The statutory and operating framework of counterpart organizations in Arizona, Colorado, Nebraska, Oregon, and South Dakota are compared in this report. Significant differences in procedures for creation and termination, selection of officers, powers and legal rights, opportunity for input to policy formulation, sources of financiing, planning responsibility, and coordination are identified. Interstate comparisons provide the backdrop for more specific examination of the interactions of districts in Utah with other organizations and agencies. The results indicate that districts have tended to embrace large scale projects as solutions to projected water shortages. The continuing and long term financial obligation constrains the districts flexibility to adjust to alternative supply options that may become visible to retail users as demand patterns change during the drawn out construction schedules of large projects. Because the Conneville Unit of the Central Utah Project is presently engaged in a large and active investigative and construction program, and is negotiating water purchase contracts, examples of some of the kinds of impediments to effective institutional interaction were more readily identified in that region by those interviewed. In situations where institutional differences occur, their mediation could be more readily effected if districts were more directly linked to general purpose government and particularly to state oversight. State government might promote more harmonious coordination of district operations by inviting periodic full and open appraisal of district plans and policies in a search for mid-course corrections that might better serve the public interest without abrogating contractual commitments