MASH1 activates expression of the paired homeodomain transcription factor Phox2a, and couples pan-neuronal and subtype-specific components of autonomic neuronal identity

We have investigated the genetic circuitry underlying the determination of neuronal identity, using mammalian peripheral autonomic neurons as a model system. Previously, we showed that treatment of neural crest stem cells (NCSCs) with bone morphogenetic protein-2 (BMP-2) leads to an induction of MASH1 expression and consequent autonomic neuronal differentiation. We now show that BMP2 also induces expression of the paired homeodomain transcription factor Phox2a, and the GDNF/NTN signalling receptor tyrosine kinase c-RET. Constitutive expression of MASH1 in NCSCs from a retroviral vector, in the absence of exogenous BMP2, induces expression of both Phox2a and c-RET in a large fraction of infected colonies, and also promotes morphological neuronal differentiation and expression of pan-neuronal markers. In vivo, expression of Phox2a in autonomic ganglia is strongly reduced in Mash1 -/- embryos. These loss- and gain-of-function data suggest that MASH1 positively regulates expression of Phox2a, either directly or indirectly. Constitutive expression of Phox2a, by contrast to MASH1, fails to induce expression of neuronal markers or a neuronal morphology, but does induce expression of c-RET. These data suggest that MASH1 couples expression of pan-neuronal and subtype-specific components of autonomic neuronal identity, and support the general idea that identity is established by combining subprograms involving cascades of transcription factors, which specify distinct components of neuronal phenotype

Comparison of the generic neuronal differentiation and neuron subtype specification functions of mammalian achaete-scute and atonal homologs in cultured neural progenitor cells

In the vertebrate peripheral nervous system, the proneural genes neurogenin 1 and neurogenin 2 (Ngn1 and Ngn2), and Mash1 are required for sensory and autonomic neurogenesis, respectively. In cultures of neural tube-derived, primitive PNS progenitors NGNs promote expression of sensory markers and MASH1 that of autonomic markers. These effects do not simply reflect enhanced neuronal differentiation, suggesting that both bHLH factors also specify neuronal identity like their Drosophila counterparts. At high concentrations of BMP2 or in neural crest stem cells (NCSCs), however, NGNs like MASH1 promote only autonomic marker expression. These data suggest that that the identity specification function of NGNs is more sensitive to context than is that of MASH1. In NCSCs, MASH1 is more sensitive to Notch-mediated inhibition of neurogenesis and cell cycle arrest, than are the NGNs. Thus, the two proneural genes differ in other functional properties besides the neuron subtype identities they can promote. These properties may explain cellular differences between MASH1- and NGN-dependent lineages in the timing of neuronal differentiation and cell cycle exit

Personal Goal Setting Behavior and Professional Outlooks of Academic Library Employees

Studies indicate correlations between personal goal-setting behaviors, performance and attitude in professional roles. An online study was developed and conducted in 2016 with academic library employees to better understand the goal-setting behavior of library employees in a particular context, via setting New Year’s resolutions, which is defined as a decision to do something or to behave in a certain manner . Results show that nearly half (49.6 percent) of all respondents set New Year’s resolutions in 2016. Goals related to health and fitness topped the list of goals that were set, followed by occupational goals. Of those who felt unclear about their purpose in life, 57 percent felt they were somewhat to very likely able to accomplish their top goals. Comparatively, 82 percent of those who had a clear sense of purpose in life felt the same

New Year\u27s Resolutions, Career Outlook, and Personality: An Investigation of Library Employees\u27 Goal Setting Behaviors

Many organizations use goal setting as a professional tool to get employees to achieve gains within the workplace. Many librarians in the United States participate in setting New Year\u27s resolutions as part of their personal goal-setting behavior. As studies indicate correlations between personal goal-setting behaviors, performance and attitude in professional roles, the researchers posit that learning how library employees set and achieve New Year\u27s resolutions could provide insight into how organizations can improve employee goal setting. An online survey was conducted in 2016 with 512 respondents. Results showed that respondents who self-reported as ambitious tend to be more successful in achieving their goals. Furthermore, ambitious respondents utilized goal-setting best practices more than less ambitious respondents. Respondents with a less clear sense of purpose in life tended to put in less effort or gave up more often than those with a clearer sense of purpose

Distinct subpopulations of enteric neuronal progenitors defined by time of development, sympathoadrenal lineage markers and Mash-1-dependence

Enteric and sympathetic neurons have previously been proposed to be lineally related. We present independent lines of evidence that suggest that enteric neurons arise from at least two lineages, only one of which expresses markers in common with sympathoadrenal cells. In the rat, sympathoadrenal markers are expressed, in the same order as in sympathetic neurons, by a subset of enteric neuronal precursors, which also transiently express tyrosine hydroxylase. If this precursor pool is eliminated in vitro by complement-mediated lysis, enteric neurons continue to develop; however, none of these are serotonergic. In the mouse, the Mash-1−/− mutation, which eliminates sympathetic neurons, also prevents the development of enteric serotonergic neurons. Other enteric neuronal populations, however, including those that contain calcitonin gene related peptide are present. Enteric tyrosine hydroxylase-containing cells co-express Mash-1 and are eliminated by the Mash-1−/− mutation, consistent with the idea that in the mouse, as in the rat, these precursors generate serotonergic neurons. Serotonergic neurons are generated early in development, while calcitonin gene related peptide-containing enteric neurons are generated much later. These data suggest that enteric neurons are derived from at least two progenitor lineages. One transiently expresses sympathoadrenal markers, is Mash-1-dependent, and generates early-born enteric neurons, some of which are serotonergic. The other is Mash-1-independent, does not express sympathoadrenal markers, and generates late-born enteric neurons, some of which contain calcitonin gene related peptide

Chlorophyll fluorescence data reveals climate-related photosynthesis seasonality in Amazonian forests

This is the final version of the article. Available from MDPI via the DOI in this record.Amazonia is theworld largest tropical forest, playing a key role in the global carbon cycle. Thus, understanding climate controls of photosynthetic activity in this region is critical. The establishment of the relationship between photosynthetic activity and climate has been controversial when based on conventional remote sensing-derived indices. Here, we use nine years of solar-induced chlorophyll fluorescence (ChlF) data from the Global Ozone Monitoring Experiment (GOME-2) sensor, as a direct proxy for photosynthesis, to assess the seasonal response of photosynthetic activity to solar radiation and precipitation in Amazonia. Our results suggest that 76% of photosynthesis seasonality in Amazonia is explained by seasonal variations of solar radiation. However, 13% of these forests are limited by precipitation. The combination of both radiation and precipitation drives photosynthesis in the remaining 11% of the area. Photosynthesis tends to rise only after radiation increases in 61% of the forests. Furthermore, photosynthesis peaks in the wet season in about 58% of the Amazon forest. We found that a threshold of ≈1943 mm per year can be defined as a limit for precipitation phenological dependence. With the potential increase in the frequency and intensity of extreme droughts, forests that have the photosynthetic process currently associated with radiation seasonality may shift towards a more water-limited system.We gratefully acknowledge the CAPES and FAPESP (Grants No. 13/14520-6 and No. 2013/50533-5) agencies for providing research fellowships and support this work. L.O.A and L.E.O.C.A thank the National Council for Scientific and Technological Development (CNPq), for the productivity fellowship, processes number 309247/2016-0 and 305054/2016-3, respectively. F.H.W. have been funded by the FAPESP (process number 13/14520-6, process number 15/50484-0 and process number 16/17652-9)

Isolating Neighborhood Trajectory Computations in Non-Autonomous Systems Including the Elliptic Restricted Three-Body Problem

Isolating block and isolating neighborhood methods have previously been implemented to find transit trajectories and orbits around libration points in the autonomous circular restricted three-body problem. For some applications, the direct computation of these types of trajectories in non-autonomous models more closely approximating real-world ephemerides is beneficial. Here, we apply isolating neighborhood methods to non-autonomous systems, including the elliptic restricted three-body problem (ERTBP). Specifically, simplified isolating neighborhood boundaries are computed around libration points in the ERTBP. These boundaries are used in combination with a bisection method to compute the forward asymptotic trajectories of the isolated invariant set and track orbits around a libration point

An Interactive Power System Analyzer With Graphics Display For Educational Use

The paper presents a student-oriented power-system analyzer with an interactive graphics display. Its capabilities include studies of (1) load flow, (2) load and generation, (3) voltage level control, (4) economic dispatch, and (5) contingency and planning analysis. Power network configurations of up to 30 buses can be presented clearly on one screen. The interactive nature of this program makes such studies effective and easy to use. With the support of a computer with graphics software and many graphics terminals, this program can be a useful teaching tool for power system studies. Copyright © 1986 by The Institute of Electrical and Electronics Engineers, Inc

Increased wildfire risk driven by climate and development interactions in the Bolivian Chiquitania, Southern Amazonia.

This is the final version of the article. Available from the publisher via the DOI in this record.Wildfires are becoming increasingly dominant in tropical landscapes due to reinforcing feedbacks between land cover change and more severe dry conditions. This study focused on the Bolivian Chiquitania, a region located at the southern edge of Amazonia. The extensive, unique and well-conserved tropical dry forest in this region is susceptible to wildfires due to a marked seasonality. We used a novel approach to assess fire risk at the regional level driven by different development trajectories interacting with changing climatic conditions. Possible future risk scenarios were simulated using maximum entropy modelling with presence-only data, combining land cover, anthropogenic and climatic variables. We found that important determinants of fire risk in the region are distance to roads, recent deforestation and density of human settlements. Severely dry conditions alone increased the area of high fire risk by 69%, affecting all categories of land use and land cover. Interactions between extreme dry conditions and rapid frontier expansion further increased fire risk, resulting in potential biomass loss of 2.44±0.8 Tg in high risk area, about 1.8 times higher than the estimates for the 2010 drought. These interactions showed particularly high fire risk in land used for 'extensive cattle ranching', 'agro-silvopastoral use' and 'intensive cattle ranching and agriculture'. These findings have serious implications for subsistence activities and the economy in the Chiquitania, which greatly depend on the forestry, agriculture and livestock sectors. Results are particularly concerning if considering the current development policies promoting frontier expansion. Departmental protected areas inhibited wildfires when strategically established in areas of high risk, even under drought conditions. However, further research is needed to assess their effectiveness accounting for more specific contextual factors. This novel and simple modelling approach can inform fire and land management decisions in the Chiquitania and other tropical forest landscapes to better anticipate and manage large wildfires in the future.The author(s) received no specific funding for this research. The study was mostly self-funded by the corresponding author TD as part of her PhD thesis. TD was supported by the Santander Academic Travel Award to visit INPE as part of this study

Experimental investigation of optical atom traps with a frequency jump

We study the evolution of a trapped atomic cloud subject to a trapping frequency jump for two cases: stationary and moving center of mass. In the first case, the frequency jump initiates oscillations in the cloud's momentum and size. At certain times we find the temperature is significantly reduced. When the oscillation amplitude becomes large enough, local density increases induced by the anharmonicity of the trapping potential are observed. In the second case, the oscillations are coupled to the center of mass motion through the anharmonicity of the potential. This induces oscillations with even larger amplitudes, enhancing the temperature reduction effects and leading to nonisotropic expansion rates while expanding freely.Comment: 8 figures, Journal of Physics B: At. Mol. Op. Phy