Subrings of singular cohomology associated to spectra

This paper extends the relation established for group cohomology by Green, Hunton and Schuster between chromatic phenomena in stable homotopy theory and certain natural subrings of singular cohomology. This exploits the theory due to Henn, Lannes and Schwartz of unstable algebras over the Steenrod algebra localized away from nilpotents.Comment: This is the version published by Algebraic & Geometric Topology on 24 August 200

Induction of autophagy markers is associated with attenuation of miR-133a in diabetic heart failure patients undergoing mechanical unloading.

Autophagy is ubiquitous in all forms of heart failure and cardioprotective miR-133a is attenuated in human heart failure. Previous reports from heart failure patients undergoing left ventricular assist device (LVAD) implantation demonstrated that autophagy is upregulated in the LV of the failing human heart. Studies in the murine model show that diabetes downregulates miR-133a. However, the role of miR-133a in the regulation of autophagy in diabetic hearts is unclear. We tested the hypothesis that diabetes exacerbates cardiac autophagy by inhibiting miR-133a in heart failure patients undergoing LVAD implantation. The miRNA assay was performed on the LV of 15 diabetic (D) and 6 non-diabetic (ND) heart failure patients undergoing LVAD implantation. Four ND with highly upregulated and 5 D with highly downregulated miR-133a were analyzed for autophagy markers (Beclin1, LC3B, ATG3) and their upstream regulators (mTOR and AMPK), and hypertrophy marker (beta-myosin heavy chain) by RT-qPCR, Western blotting and immunofluorescence. Our results demonstrate that attenuation of miR-133a in diabetic hearts is associated with the induction of autophagy and hypertrophy, and suppression of mTOR without appreciable difference in AMPK activity. In conclusion, attenuation of miR-133a contributes to the exacerbation of diabetes mediated cardiac autophagy and hypertrophy in heart failure patients undergoing LVAD implantation

Malondialdehyde Acetaldehyde Adducts (MAA-Adducts) Direct Distinctive Pro-Inflammatory Responses in Endothelial and Macrophage Cell Lines

Chronic inflammation plays a critical role in the pathogenesis of atherosclerosis. At present, the mechanism(s) by which inflammation contributes to this disease isnot entirely understood. Inflammation is known to induce oxidative stress, of which one consequence is lipid peroxidation. This process leads to the production of malondialdehyde (MDA), which can subsequently break down to form acetaldehyde (AA). These two aldehyde by-products can covalently interact with the ε-amino group of lysineswithin proteins and lipoproteins leading to the formation of highly immunogenic malondialdehyde-acetaldehyde adducts (MAA-adducts). The aim of this study was to determine the in-vitro cytokine response of endothelial cells and macrophages treated with MAA-modified human serum albumin (HSA-MAA) and low-density lipoprotein (LDL-MAA). In addition, cells isolated from mice with exposure to MAA and high fat diets were stained and imaged for uptake of the modified macromolecules of interest. We found that exposure of endothelial cells resulted in increased expression of IL-6, TNF-α, ICAM-1, VCAM-1, and MCP-1 in response to incubation with HSA-MAA; whereas, the same treatment of macrophages resulted in increased expression of IL-6, TNF-α, and IL-1b. LDL-MAA incubationresulted in increased TNF-α expression in macrophages, but MCP-1 was elevated in endothelial cells. Interestingly, the quantitative and qualitative uptake of triglycerides was increased in both endothelial and macrophage cells when exposed to LDL-MAA compared to LDL alone. The results of these studies demonstrate that different MAA-adducts elicit unique responses in different cell types. Additionally, the presence of MAA appears to modulate the cells leading to increased uptake of triglycerides and further progression of the inflammatory response.https://digitalcommons.unmc.edu/emet_posters/1003/thumbnail.jp

Unplanned readmissions after hospital discharge among patients identified as being at high risk for readmission using a validated predictive algorithm

BACKGROUND: Unplanned hospital readmissions are common, expensive and often preventable. Strategies designed to reduce readmissions should target patients at high risk. The purpose of this study was to describe medical patients identified using a recently published and validated algorithm (the LACE index) as being at high risk for readmission and to examine their actual hospital readmission rates. METHODS: We used population-based administrative data to identify adult medical patients discharged alive from 6 hospitals in Toronto, Canada, during 2007. A LACE index score of 10 or higher was used to identify patients at high risk for readmission. We described patient and hospitalization characteristics among both the high-risk and low-risk groups as well as the 30-day readmission rates. RESULTS: Of 26 045 patients, 12.6% were readmitted to hospital within 30 days and 20.9% were readmitted within 90 days of discharge. High-risk patients (LACE ≥ 10) accounted for 34.0% of the sample but 51.7% of the patients who were readmitted within 30 days. High-risk patients were readmitted with twice the frequency as other patients, had longer lengths of stay and were more likely to die during the readmission. INTERPRETATION: Using a LACE index score of 10, we identified patients with a high rate of readmission who may benefit from improved post-discharge care. Our findings suggest that the LACE index is a potentially useful tool for decision-makers interested in identifying appropriate patients for post-discharge interventions

The Allen Telescope Array Search for Electrostatic Discharges on Mars

The Allen Telescope Array was used to monitor Mars between 9 March and 2 June 2010, over a total of approximately 30 hours, for radio emission indicative of electrostatic discharge. The search was motivated by the report from Ruf et al. (2009) of the detection of non-thermal microwave radiation from Mars characterized by peaks in the power spectrum of the kurtosis, or kurtstrum, at 10 Hz, coinciding with a large dust storm event on 8 June 2006. For these observations, we developed a wideband signal processor at the Center for Astronomy Signal Processing and Electronics Research (CASPER). This 1024-channel spectrometer calculates the accumulated power and power-squared, from which the spectral kurtosis is calculated post-observation. Variations in the kurtosis are indicative of non-Gaussianity in the signal, which can be used to detect variable cosmic signals as well as radio frequency interference (RFI). During the three month period of observations, dust activity occurred on Mars in the form of small-scale dust storms, however no signals indicating lightning discharge were detected. Frequent signals in the kurtstrum that contain spectral peaks with an approximate 10 Hz fundamental were seen at both 3.2 and 8.0 GHz, but were the result of narrowband RFI with harmonics spread over a broad frequency range.Comment: 26 pages, 12 figures, accepted to Ap

Probing the Gas Content of Late-stage Protoplanetary Disks with N_2H^+

The lifetime of gas in circumstellar disks is a fundamental quantity that informs our understanding of planet formation. Studying disk gas evolution requires measurements of disk masses around stars of various ages. Because H_2 gas is unobservable under most disk conditions, total disk masses are based on indirect tracers such as sub-mm dust and CO emission. The uncertainty in the relation between these tracers and the disk mass increases as the disk evolves. In a few well-studied disks, CO exhibits depletions of up to 100× below the assumed interstellar value. Thus, additional tracers are required to accurately determine the total gas mass. The relative lack of nitrogen found in solid solar system bodies may indicate that it persists in volatile form, making nitrogen-bearing species more robust tracers of gas in more evolved disks. Here we present Atacama Large Millimeter/submillimeter Array detections of N_2H^+ in two mature, ~5–11 Myr old disks in the Upper Scorpius OB Association. Such detections imply the presence of H_2-rich gas and sources of ionization, both required for N_2H^+ formation. The Upper Sco disks also show elevated N_2H^+/CO flux ratios when compared to previously observed disks with ≳10× higher CO fluxes. Based on line ratio predictions from a grid of thermochemical disk models, a significantly reduced CO/H_2 abundance of <10^(−6) for a gas-to-dust ratio of ≳100 is required to produce the observed N_2H^+ fluxes. These systems appear to maintain H_2 gas reservoirs and indicate that carbon- and nitrogen-bearing species follow distinct physical or chemical pathways as disks evolve