The FOMC’s “considerable period”

Monetary policy

Conserved Amino Acids in Each Subunit of the Heteroligomeric tRNA m\u3csup\u3e1\u3c/sup\u3eA58 Mtase from \u3cem\u3eSaccharomyces cerevisiae\u3c/em\u3e Contribute to tRNA Binding

In Saccharomyces cerevisiae, a two-subunit methyltransferase (Mtase) encoded by the essential genes TRM6 and TRM61 is responsible for the formation of 1-methyladenosine, a modified nucleoside found at position 58 in tRNA that is critical for the stability of . The crystal structure of the homotetrameric m1A58 tRNA Mtase from Mycobacterium tuberculosis, TrmI, has been solved and was used as a template to build a model of the yeast m1A58 tRNA Mtase heterotetramer. We altered amino acids in TRM6 and TRM61 that were predicted to be important for the stability of the heteroligomer based on this model. Yeast strains expressing trm6 and trm61 mutants exhibited growth phenotypes indicative of reduced m1A formation. In addition, recombinant mutant enzymes had reduced in vitro Mtase activity. We demonstrate that the mutations introduced do not prevent heteroligomer formation and do not disrupt binding of the cofactor S-adenosyl-l-methionine. Instead, amino acid substitutions in either Trm6p or Trm61p destroy the ability of the yeast m1A58 tRNA Mtase to bind , indicating that each subunit contributes to tRNA binding and suggesting a structural alteration of the substrate-binding pocket occurs when these mutations are present

Leaving an Abusive Partner: An Empirical Review of Predictors, the Process of Leaving, and Psychological Well-Being

Four facets of leaving an abusive relationship are reviewed: (a) factors related to initially leaving an abusive partner; (b) the process of leaving an abusive relationship; (c) the psychological well-being of survivors after leaving; and (d) the predictors of this well-being. The conceptual and methodological limitations of studies in each of these areas are presented. Consistently found predictors of leaving include both material and psychological factors. Because battered women typically undergo several shifts in their thinking about the abuse before leaving permanently, research on leaving as a process is highlighted. Astress-process framework is used to explain the seemingly paradoxical finding that some women just out of the abusive relationship may have greater psychological difficulties than those who are still in it. For those experiencing the most stress, psychological health can worsen over time. Researchers and practitioners need to pay more attention to the plight of women who have left abusive partners.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/90017/1/Anderson-DK-Saunders-DG-2003 Leaving an Abusive Partner TVA.pd

Excess Observed in CDF Bs0→μ+μ−B^0_s \to \mu^{+} \mu^{-} and SUSY at the LHC

The recent excess observed by CDF in $B^0_s \to \mu^{+} \mu^{-}$ is interpreted in terms of a possible supersymmetric origin. An analysis is given of the parameter space of mSUGRA and non-universal SUGRA models under the combined constraints from LHC-7 with 165 pb$^{-1}$ of integrated luminosity, under the new XENON-100 limits on the neutralino-proton spin independent cross section and under the CDF $B^0_s \to \mu^{+} \mu^{-}$ 90% C.L. limit reported to arise from an excess number of dimuon events. It is found that the predicted value of the branching ratio $B^0_s \to \mu^{+} \mu^{-}$ consistent with all the constraints contains the following set of NLSPs: chargino, stau, stop or CP odd (even) Higgs. The lower bounds of sparticles, including those from the LHC, XENON and CDF $B^0_s\to \mu^+\mu^-$ constraint, are exhibited and the shift in the allowed range of sparticle masses arising solely due to the extra constraint from the CDF result is given. It is pointed out that the two sided CDF 90% C.L. limit puts upper bounds on sparticle masses. An analysis of possible signatures for early discovery at the LHC is carried out corresponding to the signal region in $B^0_s \to \mu^{+} \mu^{-}$. Implications of GUT-scale non-universalities in the gaugino and Higgs sectors are discussed. If the excess seen by the CDF Collaboration is supported by further data from LHCb or D0, this new result could be a harbinger for the discovery of supersymmetry.Comment: References added, text update

The Translocating RecBCD Enzyme Stimulates Recombination by Directing RecA Protein onto ssDNA in a χ-Regulated Manner

AbstractDouble-stranded DNA break repair and homologous recombination in E. coli are initiated by the RecBCD enzyme, which unwinds and simultaneously degrades DNA from a double-stranded DNA end. This process is stimulated by cis-acting DNA elements, known as χ sites. Using both in vitro pairing and nuclease protection assays, we demonstrate that the translocating RecBCD enzyme, which has been activated by χ, coordinates the preferential loading of the homologous pairing protein, RecA, onto the resultant single-stranded DNA downstream of χ. This facilitated loading of RecA protein results in a substantial increase in both the efficiency and rate of in vitro recombination reactions and offers an explanation for stimulation of recombination and repair in vivo by χ

Botrychium echo W.H. Wagner (reflected grapefern): a technical conservation assessment

Prepared for: the USDA Forest Service, Rocky Mountain Region, Species Conservation Project.July 22, 2004.Includes bibliographical references

Reconstitution of an SOS Response Pathway Derepression of Transcription in Response to DNA Breaks

AbstractE. coli responds to DNA damage by derepressing the transcription of about 20 genes that make up the SOS pathway. Genetic analyses have shown that SOS induction in response to double-stranded DNA (dsDNA) breaks requires LexA repressor, and the RecA and RecBCD enzymes—proteins best known for their role as initiators of dsDNA break repair and homologous recombination. Here we demonstrate that purified RecA protein, RecBCD enzyme, single-stranded DNA-binding (SSB) protein, and LexA repressor respond to dsDNA breaks in vitro by derepressing transcription from an SOS promoter. Interestingly, derepression is more rapid if the DNA containing the dsDNA break has a χ recombination hot spot (5′-GCTGGTGG-3′), suggesting a novel regulatory role for one of the most overrepresented octamers in the E. coli genome