109 research outputs found

    Review on Recent Development and Application of Dispersive Liquid-Liquid Micro Extraction

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    Dispersive liquid-liquid micro extraction (DLLME) has become a very popular environmentally benign sample-preparation technique, due to its simplicity, rapidity of operation and low consumption of solvent and reagent. It has attracted much interest from scientist working in separation science and much improvement has been made since its introduction in 2006. It has been combined with different extraction techniques such as floating organic drop, solid-phase extraction, and supercritical fluid extraction. This seminar has focused on the review on development of dispersive liquid micro extraction (DLLME) and its application in different samples, such as water, soil, food and biological material with different analytical techniques.DOI: 10.7176/CMR/11-3-02Publication date:March 31st 201

    Plant Tissue Nutrient Concentration and Major Soil Nutrients Influenced by NPSB Fertilizer Rates and Plant Population of Maize (Zea Mays L.) at Bako, Oromia National Regional State

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    Plant nutrients such as nitrogen, phosphorus, potassium and sulfur are the nutrients frequently limit the yields of maize over the world. Nutrient recommendation should be based on the optimum yield and environmental save that can be through plant tissue analysis and soil test. This experiment was conducted at Bako Agricultural Research Center west region of Ethiopia Gobu Sayo using split plot desighn with three levels of plant population 53.333,66,666 and 76,923 plants per hectare on main plot and five level 0,100,150,200,250 kg/ha of NPSB fertilizer rates on sub-plots. Soil sample was taken from the plots pre-harvesting for composite sample and post-harvest from each plot and analyzed at Bako soil laboratory and Ethiopia water work design Addis Ababa for soil fertility management. Nutrient in plant tissue was analyzed at Holota Agricultural Research Center soil laboratory. Collected data was analyzed using Genstat computer statistical soft-ware package. The result of pre planting soil analysis showed soil of the study area was categorized as clay soil with medium and stable organic matter and low organic carbon and low total nitrogen that shows low soil fertility. The ANOVA result showed addition of fertilizer reduced soil pH from 5.7 to 5.47 and bellow. 150 kg/ha of NPSB which gave economically feasible yield 9460kg/ha moderately acidified the soil. This soil analysis result showed that the level at which fertilizer boost yield of maize. Only percent of phosphorus was changed due to plant population density. Plant tissue concentration at silking was significantly influenced by fertilizer rates. Potassium is at sufficiency range for all level of supplied fertilizer. Sufficient range nitrogen at silkin was recorded from the plot received 100-150 kg/ha which gave economically feasible yield. It is better to use fertilizer rate of 150kg/ha which shows sufficiency range for nutrient concentration of soil and plant tissue in leaf at silking stage. This finding is not the end for the area and further research should be carried out for feature. Keywords:plant tissue, soil, Zea mays L., Nitrogen, Phosphorus, Sulfur, potassium DOI: 10.7176/JBAH/12-13-04 Publication date:July 31st 202

    Estimation of County-Level Diabetes Prevalence Using Bayesian Hierarchical Model

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    Because of budget constraints a survey has two major limitations when it comes to availing data on prevalence of diabetes in small areas as Counties. First, it is costly for a survey to cover all relevant areas. And second, a survey often comes short of taking large samples for adequate representations. Examining such limitations and shortcomings of a direct method of estimation which uses data from such surveys, this dissertation attempted to apply Bayesian Hierarchical Model of estimation to provide reliable data on prevalence of Diabetes in small areas (counties). In doing so a range of Bayesian Hierarchical models which provide reliable data on prevalence of diabetes for small areas as counties were explored. The Estimation Models used data of Behavioral Risk Factor Surveillance System (BRFSS [1]) survey. In total the analysis examined survey data made on 1,497 counties (including the 644 counties in the CDC diabetes belts [2] in 16 states in the US. The statistical models used in this analysis are aimed at reducing estimation error of diabetes prevalence in direct estimation methods, so as to help an efficient policy formulation and budget allocation. In this regard we generated estimates on the prevalence of diabetes for 1,188 Counties having a complete set of information and another 295 which were not covered in BRFSS survey and among the 1188 Counties 824 Counties that have smaller sample size (Healthy people 2020 data suppression for BRFSS [3]). Unlike the direct method usually applied for such estimation the result in this analysis brought about statistical significance of the estimates in our study

    Genetic analysis of earliness traits in chickpea (Cicer arietinum L.)

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    The latter part of the reproductive growth phase in chickpea (Cicer arietinum L.) often coincides with declining temperature and wet conditions in western Canada, in sharp contrast to many other growing environments. This exacerbates the indeterminate nature of the crop, leading to excessive canopy development, and subsequently resulting in delayed maturity. The objectives of this study were to: i) determine the genetic relationships of short internode, double podding and early flowering traits with earliness of crop maturity; ii) determine the genetic control of major earliness traits in chickpea; iii) assess the patterns of post-flowering dry matter accumulation and partitioning to reproductive parts as related to earliness. The results showed that double podding significantly reduced the number of days taken to maturity, under the conditions where this trait was sufficiently expressed. The best double podding genotypes, i.e. those with 15—35% of the podded nodes bearing double pods, were about one week earlier than their single podding counterparts and standard checks. A physiological study revealed that the double podding parental genotype 272-2 partitioned a relatively greater proportion (about 58%) of the total dry matter to pods compared to 42—54% in the single podding genotypes. Double podding increased the total number of pods set, and thus the increased demand for assimilates may have precluded further production of stems and leaves, resulting in an earlier transition of reproductive growth to physiological maturity. Days to flowering was positively associated with days to maturity, and partial path analysis revealed that days to flowering contributed to days to maturity indirectly via days to first pod maturity. Days to flowering explained 32% of the variation in days to first pod maturity. However, the short internode trait had an undesirable effect, in that all the short internode segregants were too late to mature. Genetic studies revealed that days to flowering was determined by two major genes plus polygenes in chickpea in the short-season temperate environment of western Canada. The two major genes control over 65% of the phenotypic variation. Also, the additive component of genetic variance was significant for days to first podding, days to first pod maturity, reproductive period, and days to maturity; which is desirable for development of superior inbred cultivars of chickpea. These key phenological traits are interrelated but could be manipulated separately in the breeding process. Additional gain in earliness of crop maturity may be achieved through combined selection for these traits

    Response of Yield and Yield Components of Tef [Eragrostis Tef (Zucc.) Trotter] to Optimum Rates of Nitrogen and Phosphorus Fertilizer Rate Application in Assosa Zone, Benishangul Gumuz Region

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     አህፅርኦት ይህ የመስክ ላይ ሙከራ በቤንሻንጉል ጉሙዝ ክልል በአሶሳ ዞን በአሶሳና ባምባስ ወረዳ በአርሶ አደር ማሳዎች ላይ ሇሁሇት ተከታታይ ዓመታት (ከ2005 – 2006 ዓ.ም) የተከናወነ ሲሆን ዋና )ዓላማዉም የናይትሮጅንና የፎስፈረስ ማዳበሪያ ላይ ያላቸዉን ተጽዕኖ መገምገም ነበር፡፡ ይህንንም ሇማጥናት አምስት(5) የናይትሮጅን እና አራት(4) የፎስፈረስ ደረጃዎች ድብልቅ በአጠቃላይ ሀያ (20) ተጠኚዎችን በFactorial combination RCBD ዲዛይንና በሦስት ድግግምሽ ተሞክሯል፡፡ ዉጤቱ እንደሚያሳየዉ የጤፍ ዕድገትና ምርት በናይትሮጂንና በፎስፈረስ ማዳበሪያ መጠቀም አመርቂ ሇዉጥ አሳይቷል፡፡ በመሆኑም 46 ኪ.ግ ናይትሮጅን እና 10 ኪ.ግ ፎስፈረስ በሄክታር በመጠቀም 1681.1 ኪ.ግ የጤፍ ምርት በሄክታር ተገኝቷል፡፡ ይህ የማዳበሪያ መጠን ምንም ማዳበሪያ ካልተጨመረበት ጋር ሲነፃፀር በ137% የምርት ጭማሪን ያስገኛል፡፡ የዋጋ አዋጭነትን ስንመሇከትም ይህ የማዳበሪያ መጠን አዋጭ መሆኑን ያረጋግጣል፡፡ ስሇዚህም 46 ኪ.ግ ናይትሮጅን እና 10 ኪ.ግ ፎስፈረስ በሄክታር መጠቀም ከፍተኛ የሆነ ትርፍ ያሇዉና አዋጭ የማደበሪያ አጠቃቀም መሆኑ ተረጋግጧል፡፡ AbstractTef (Eragrostis tef) is one of most important food crops grown in Benishangul Gumuz region of Ethiopia and is produced in different agro-ecologies in the region. However, its productivity is constrained by a number of problems, of which soil nutrient is the most important one. Information on the response of tef to Nitrogen (N) and Phosphorus (P) fertilizers in Assosa Zone is inadequate. Field experiments were conducted at two locations for two years (2012-2013) to investigate the response of tef to N and P fertilization. Five levels of nitrogen (0, 23, 46, 69 and 92 N kg ha–1) and four levels of phosphorus (0, 10, 20 and 30 kg P ha–1) were studied in factorial combinations in a randomized complete block design (RCBD) with three replications. The results revealed substantial responses of tef to the main effect of N and P on days to heading and panicle length, the main effect of N on days to emergency, and the main effect of P on days to 90% physiological maturity. Nitrogen by P interaction effect was significant on plant height, straw and grain yield. Grain yield increased significantly from 708.6 to 1681.1 kg ha-1 with increase in the level of N and P from the control (0/0 N/P) to 46 kg N and 10 P kg ha-1. The magnitude of increase in grain yield due to application of 46 kg N along and 10 kg P ha -1 was 137 % higher as compared to the control. The partial budget analysis also indicates that applications of 46 kg N ha-1 and 10 kg P ha-1 are the most economical fertilizer rates to tef growers compared to the other levels in the study area

    Stabilizing yield and quality: early maturing chickpea for the prairies

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    Non-Peer ReviewedProgress in chickpea breeding has been constrained by lack of good sources of early maturity in the short-season temperate environment of western Canada. We hypothesized that the length of the chickpea life-cycle could be reduced through introgression of strategic genetic traits including short internode, double podding and early flowering. The result showed that both the double podding and early flowering traits had significant beneficial effects by reducing the duration of crop maturity in chickpea in the short-season temperate environment of western Canada. Pyramiding double podding, early flowering and other strategic genetic traits should lead to the development of extra short duration chickpea varieties more suited for cultivation in the Prairies and similar environments

    Early maturity strategies in chickpea: pyramiding key genetic traits

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    Non-Peer ReviewedChickpea (Cicer arietinum L.) is a highly indeterminate species which continues to grow and produce pods through fall, especially in cooler and wetter seasons. This exposes the crop to frost damage resulting in reduced yield and quality. We hypothesized that earliness could be induced through some strategic traits including double podding, reduced internode length and early flowering. The objective of this study was, therefore, to determine the individual and combined effects of these traits on earliness in chickpea. Four single crosses were made: two with the early flowering parent, and one each with the double podding and short internode length parents. Segregating populations of F2–F4 generations were evaluated along with their parents in greenhouse and field trials. Significant genetic variability was observed for days to maturity of lower pods and percent pod maturity at four months after planting. In general, each trait had some effect on earliness, though the magnitude differed, and combining them into a single genotype may help to achieve the desired earliness in chickpea. The F2 population from the multiple cross (gene pyramiding) showed a wide range in maturity. Several had nearly complete maturity of pods at four months after planting. These were more determinate types as judged by smaller increases in height between flowering and maturity stages and uniform maturity of pods

    Early flowering, short internode length, and double podding as components of early maturity in chickpea

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    Non-Peer ReviewedLate maturity, resulting from the indeterminate nature of the crop, is a critical production constraint in chickpea (Kumar and Abbo, 2001). In western Canada, the chickpea crop is often killed by fall frost while seeds/pods are yet filling, which reduces both the quantity and quality of economic yield (grain). It was hypothesized that incorporating early flowering, short internode length and double podding traits may shorten the overall duration of growth in chickpea. Early flowering would advance onset of reproduction, while the latter two traits hasten the rate of pod formation and then progress towards maturity. Hence, a study was initiated to determine the effect of these key traits on time (days) to maturity in chickpea

    Impact of grasspea genotypes and sowing dates on seed &#946-ODAP concentration and agronomic traits.

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    Grasspea (Lathyrus sativus) is an important food legume crop in Ethiopia. However, its nutritional value ishindered by β-ODAP that causes lathyrism in humans. The extent of toxicity is influenced by genetic andagronomic factors.We conducted an experiment to determine the effect of varieties and sowing dates on the β-ODAP content of the seeds and other yield components. Two varieties, Bio-520 and landrace, were planted onJuly 24, August 7 and 21, and September 4 in the 2001-02 and 2002-03 cropping seasons. Grasspea variety andseason, significantly influenced β-ODAP content of the seeds.The highly significant variety x season interactionssuggests that varieties behave differently in different seasons for their β-ODAP content. The main effects andinteractions were highly significant for days-to-flowering and plant height. Although β-ODAP content of thevarieties varied with season, the improved genotype always contained less β-ODAP concentration.Thus, whilethe development of low-toxin grasspea lines is the primary goal, modifying agronomic practices is also importantto mitigate lathyrism.Key Words: Ethiopia, Lathyrus sativus, Lathyrism, neurotoxi
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