248 research outputs found
A conversação na entrevista de perfil na mídia escrita: uma questão para o ensino
Profile interviews are frequent conversational events in the media. In education, as an object of study, it fosters the development of a critical perception of verbal interactions occurring in social communication. In this article, we present the results of an analysis carried out with an interview published in the Yellow Pages section of Veja Magazine, on October 20th, 2004. Our goal is to examine the textual-discursive strategies behind such genre rendering. Tents of Conversation Analysis and Interactional sociolinguistics will give support to the reflections proposed.A entrevista de perfil é um evento conversacional frequente na mídia. No ensino, como objeto de estudo, vem favorecer o desenvolvimento de uma percepção crítica da interação verbal que ocorre na comunicação social. Neste artigo, procedemos à análise de uma entrevista publicada na Revista Veja, na seção Páginas Amarelas, em 20/10/2004, visando à discussão das estratégias textuais-discursivas de produção do gênero. Preceitos da Análise da Conversação e da Sociolinguística Interacional darão suporte às reflexões
A palavra na literatura e em outras artes
O presente número da Revista FronteiraZ traz um dossiê de artigos reunidos sob a temática A palavra na literatura e em outras artes. A literatura, como repositório das experiências humanas vividas e imaginadas, potencializa a palavra dando-lhe corporeidade. Matéria prima dos diálogos, produzidos com propósitos estéticos, aproxima-se da expressividade natural da fala e se abre a diferentes contextos de práticas interacionais. A palavra escrita, lida, vocalizada, ouvida é franqueada às diversas artes. Nas Artes Cênicas, ganha as dimensões do próprio corpo, de seu saber sensível, no trabalho do ator em seu processo de composição poética da voz. Em Artes outras, para além das literárias e performáticas, a palavra compõe visualidades e demarca sua expansão para os mais amplos territórios de manifestação estética.
Os artigos que FronteiraZ acolhe neste número flagram a expressividade da palavra em diversas manifestações artísticas, analisando-a à luz de sólidos aportes teóricos que orientam e embasam as reflexões
Noscapine acts as a protease inhibitor of in vitro elastase-induced collagen deposition in equine endometrium
Research Areas: Biochemistry & Molecular Biology ; ChemistryABSTRACT - Endometrosis is a reproductive pathology that is responsible for mare infertility. Our
recent studies have focused on the involvement of neutrophil extracellular traps enzymes, such as
elastase (ELA), in the development of equine endometrosis. Noscapine (NOSC) is an alkaloid derived
from poppy opium with anticough, antistroke, anticancer, and antifibrotic properties. The present
work investigates the putative inhibitory in vitro effect of NOSC on collagen type I alpha 2 chain
(COL1A2) mRNA and COL1 protein relative abundance induced by ELA in endometrial explants
of mares in the follicular or mid-luteal phases at 24 or 48 h of treatment. The COL1A2 mRNA was
evaluated by qPCR and COL1 protein relative abundance by Western blot. In equine endometrial
explants, ELA increased COL 1 expression, while NOSC inhibited it at both estrous cycle phases
and treatment times. These findings contribute to the future development of new endometrosis
treatment approaches. Noscapine could be a drug capable of preventing collagen synthesis in mare’s
endometrium and facilitate the therapeutic approach.info:eu-repo/semantics/publishedVersio
Collagen and microvascularization in placentas from young and older mares
Research Areas: Veterinary SciencesIn older mares, increasing collagen fibers (fibrosis) in the endometrium and oviduct
predisposes to sub-fertility and infertility. In this study, (i) gene transcription of collagen
(qPCR: COL1A1, COL1A2, COL3A1, COL5A1); (ii) total collagen protein (hydroxyproline);
(iii) collagen distribution (Picrosirius red staining; polarized light microscopy); and (iv)
microvascular density (Periodic acid-Schiff staining), were evaluated in mares’ placenta,
and related to mares age, and placenta and neonate weights. Samples were collected
from the gravid horn, non-gravid horn, and body of the placenta from younger
(n = 7), and older mares (n = 9) of different breeds. Transcripts of COL1A1, COL3A1
and COL5A1, total collagen protein, chorionic plate connective tissue thickness, and
microvascularization increased in the gravid horn of older mares’ placentas, compared
to the youngest (P < 0.05). Although in other species placenta fibrosis may indicate
placental insufficiency and reduced neonate weight, this was not observed here. It
appears that older fertile mares, with more parities, may develop a heavier, more
vascularized functional placenta with more collagen, throughout a longer gestation, which
enables the delivery of heavier foals. Thus, these features might represent morphological
and physiological adaptations of older fertile mares’ placentas to provide the appropriate
nutrition to the equine fetus.info:eu-repo/semantics/publishedVersio
The inhibitory effect of noscapine on the in vitro cathepsin G-induced collagen expression in equine endometrium
Research Areas: Life Sciences & Biomedicine ; MicrobiologyABSTRACT - Cathepsin G (CAT) is a protease released by neutrophils when forming neutrophil extracellular traps that was already associated with inducing type I collagen (COL1) in equine endometrium
in vitro. Endometrosis is a fibrotic condition mainly characterized by COL1 deposition in the equine
endometrium. The objective was to evaluate if noscapine (an alkaloid for cough treatment with
anti-neoplastic and anti-fibrotic properties) would reduce COL1A2 transcription (evaluated by qPCR)
and COL1 protein relative abundance (evaluated by western blot) induced by CAT in equine endometrial explants from follicular and mid-luteal phases treated for 24 or 48 h. The explants treated with
CAT increased COL1 expression. Noscapine decreased COL1A2 transcription at both estrous cycle
phases, but COL1 relative protein only at the follicular phase, both induced by CAT. Additionally,
the noscapine anti-fibrotic action was found to be more effective in the follicular phase. The CAT
treatment caused more fibrosis at the longest period of treatment, while noscapine acted better at the
shortest time of treatment. Our results showed that noscapine could act as an anti-fibrotic drug in
equine endometrosis by inhibiting CAT in vitro. Noscapine offers a new promising therapeutic tool
for treating fibrosis as a single non-selective agent to be considered in the future.info:eu-repo/semantics/publishedVersio
The In vitro inhibitory effect of sivelestat on elastase induced collagen and metallopeptidase expression in equine endometrium
Research Areas: Agriculture ; Veterinary Sciences ; ZoologyAbstract: Neutrophil extracellular traps (NETs) fight endometritis, and elastase (ELA), a protease
found in NETs, might induce collagen type I (COL1) accumulation in equine endometrium.
Metallopeptidases (MMPs) are involved in extracellular matrix balance. The aim was to evaluate
the e ects of ELA and sivelestat (selective elastase inhibitor) on MMP-2 and MMP-9 expression and
gelatinolytic activity, as well as the potential inhibitory e ect of sivelestat on ELA-induced COL1 in
equine endometrium. Endometrial explants from follicular (FP) and mid-luteal (MLP) phases were
treated for 24 or 48 h with ELA, sivelestat, and their combination. Transcripts of COL1A2, MMP2, and
MMP9 were evaluated by qPCR; COL1 protein relative abundance by Western blot, and MMP-2 and
MMP-9 gelatinolytic activity by zymography. In response to ELA treatment, there was an increase in
MMP2 mRNA transcription (24 h) in active MMP-2 (48 h), both in FP, and in MMP9 transcripts in FP
(48 h) and MLP (24 h) (p < 0.05). Sivelestat inhibited ELA-induced COL1A2 transcripts in FP (24 h)
and MLP (24 h, 48 h) (p < 0.05). The sivelestat inhibitory e ect was detected in MMP9 transcripts
in FP at 48 h (p < 0.05), but proteases activity was unchanged. Thus, MMP-2 and MMP-9 might be
implicated in endometrium fibrotic response to ELA. In mare endometrium, sivelestat may decrease
ELA-induced COL1 deposition and hinder endometrosis development.info:eu-repo/semantics/publishedVersio
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