A conversação na entrevista de perfil na mídia escrita: uma questão para o ensino

Profile interviews are frequent conversational events in the media. In education, as an object of study, it fosters the development of a critical perception of verbal interactions occurring in social communication. In this article, we present the results of an analysis carried out with an interview published in the Yellow Pages section of Veja Magazine, on October 20th, 2004. Our goal is to examine the textual-discursive strategies behind such genre rendering.  Tents of Conversation Analysis and Interactional sociolinguistics will give support to the reflections proposed.A entrevista de perfil é um evento conversacional frequente na mídia. No ensino, como objeto de estudo, vem favorecer o desenvolvimento de uma percepção crítica da interação verbal que ocorre na comunicação social. Neste artigo, procedemos à análise de uma entrevista  publicada na Revista Veja, na seção Páginas Amarelas, em 20/10/2004, visando à discussão das estratégias textuais-discursivas de produção do gênero. Preceitos da Análise da Conversação e da Sociolinguística Interacional darão suporte às reflexões

A palavra na literatura e em outras artes

O presente número da Revista FronteiraZ traz um dossiê de artigos reunidos sob a temática A palavra na literatura e em outras artes. A literatura, como repositório das experiências humanas vividas e imaginadas, potencializa a palavra dando-lhe corporeidade. Matéria prima dos diálogos, produzidos com propósitos estéticos, aproxima-se da expressividade natural da fala e se abre a diferentes contextos de práticas interacionais. A palavra escrita, lida, vocalizada, ouvida é franqueada às diversas artes. Nas Artes Cênicas, ganha as dimensões do próprio corpo, de seu saber sensível, no trabalho do ator em seu processo de composição poética da voz. Em Artes outras, para além das literárias e performáticas, a palavra compõe visualidades e demarca sua expansão para os mais amplos territórios de manifestação estética. Os artigos que FronteiraZ acolhe neste número flagram a expressividade da palavra em diversas manifestações artísticas, analisando-a à luz de sólidos aportes teóricos que orientam e embasam as reflexões

Noscapine acts as a protease inhibitor of in vitro elastase-induced collagen deposition in equine endometrium

Research Areas: Biochemistry & Molecular Biology ; ChemistryABSTRACT - Endometrosis is a reproductive pathology that is responsible for mare infertility. Our recent studies have focused on the involvement of neutrophil extracellular traps enzymes, such as elastase (ELA), in the development of equine endometrosis. Noscapine (NOSC) is an alkaloid derived from poppy opium with anticough, antistroke, anticancer, and antifibrotic properties. The present work investigates the putative inhibitory in vitro effect of NOSC on collagen type I alpha 2 chain (COL1A2) mRNA and COL1 protein relative abundance induced by ELA in endometrial explants of mares in the follicular or mid-luteal phases at 24 or 48 h of treatment. The COL1A2 mRNA was evaluated by qPCR and COL1 protein relative abundance by Western blot. In equine endometrial explants, ELA increased COL 1 expression, while NOSC inhibited it at both estrous cycle phases and treatment times. These findings contribute to the future development of new endometrosis treatment approaches. Noscapine could be a drug capable of preventing collagen synthesis in mare’s endometrium and facilitate the therapeutic approach.info:eu-repo/semantics/publishedVersio

Collagen and microvascularization in placentas from young and older mares

Research Areas: Veterinary SciencesIn older mares, increasing collagen fibers (fibrosis) in the endometrium and oviduct predisposes to sub-fertility and infertility. In this study, (i) gene transcription of collagen (qPCR: COL1A1, COL1A2, COL3A1, COL5A1); (ii) total collagen protein (hydroxyproline); (iii) collagen distribution (Picrosirius red staining; polarized light microscopy); and (iv) microvascular density (Periodic acid-Schiff staining), were evaluated in mares’ placenta, and related to mares age, and placenta and neonate weights. Samples were collected from the gravid horn, non-gravid horn, and body of the placenta from younger (n = 7), and older mares (n = 9) of different breeds. Transcripts of COL1A1, COL3A1 and COL5A1, total collagen protein, chorionic plate connective tissue thickness, and microvascularization increased in the gravid horn of older mares’ placentas, compared to the youngest (P < 0.05). Although in other species placenta fibrosis may indicate placental insufficiency and reduced neonate weight, this was not observed here. It appears that older fertile mares, with more parities, may develop a heavier, more vascularized functional placenta with more collagen, throughout a longer gestation, which enables the delivery of heavier foals. Thus, these features might represent morphological and physiological adaptations of older fertile mares’ placentas to provide the appropriate nutrition to the equine fetus.info:eu-repo/semantics/publishedVersio

The inhibitory effect of noscapine on the in vitro cathepsin G-induced collagen expression in equine endometrium

Research Areas: Life Sciences & Biomedicine ; MicrobiologyABSTRACT - Cathepsin G (CAT) is a protease released by neutrophils when forming neutrophil extracellular traps that was already associated with inducing type I collagen (COL1) in equine endometrium in vitro. Endometrosis is a fibrotic condition mainly characterized by COL1 deposition in the equine endometrium. The objective was to evaluate if noscapine (an alkaloid for cough treatment with anti-neoplastic and anti-fibrotic properties) would reduce COL1A2 transcription (evaluated by qPCR) and COL1 protein relative abundance (evaluated by western blot) induced by CAT in equine endometrial explants from follicular and mid-luteal phases treated for 24 or 48 h. The explants treated with CAT increased COL1 expression. Noscapine decreased COL1A2 transcription at both estrous cycle phases, but COL1 relative protein only at the follicular phase, both induced by CAT. Additionally, the noscapine anti-fibrotic action was found to be more effective in the follicular phase. The CAT treatment caused more fibrosis at the longest period of treatment, while noscapine acted better at the shortest time of treatment. Our results showed that noscapine could act as an anti-fibrotic drug in equine endometrosis by inhibiting CAT in vitro. Noscapine offers a new promising therapeutic tool for treating fibrosis as a single non-selective agent to be considered in the future.info:eu-repo/semantics/publishedVersio

The In vitro inhibitory effect of sivelestat on elastase induced collagen and metallopeptidase expression in equine endometrium

Research Areas: Agriculture ; Veterinary Sciences ; ZoologyAbstract: Neutrophil extracellular traps (NETs) fight endometritis, and elastase (ELA), a protease found in NETs, might induce collagen type I (COL1) accumulation in equine endometrium. Metallopeptidases (MMPs) are involved in extracellular matrix balance. The aim was to evaluate the e ects of ELA and sivelestat (selective elastase inhibitor) on MMP-2 and MMP-9 expression and gelatinolytic activity, as well as the potential inhibitory e ect of sivelestat on ELA-induced COL1 in equine endometrium. Endometrial explants from follicular (FP) and mid-luteal (MLP) phases were treated for 24 or 48 h with ELA, sivelestat, and their combination. Transcripts of COL1A2, MMP2, and MMP9 were evaluated by qPCR; COL1 protein relative abundance by Western blot, and MMP-2 and MMP-9 gelatinolytic activity by zymography. In response to ELA treatment, there was an increase in MMP2 mRNA transcription (24 h) in active MMP-2 (48 h), both in FP, and in MMP9 transcripts in FP (48 h) and MLP (24 h) (p < 0.05). Sivelestat inhibited ELA-induced COL1A2 transcripts in FP (24 h) and MLP (24 h, 48 h) (p < 0.05). The sivelestat inhibitory e ect was detected in MMP9 transcripts in FP at 48 h (p < 0.05), but proteases activity was unchanged. Thus, MMP-2 and MMP-9 might be implicated in endometrium fibrotic response to ELA. In mare endometrium, sivelestat may decrease ELA-induced COL1 deposition and hinder endometrosis development.info:eu-repo/semantics/publishedVersio