E Actitrode: The new selective stimulation interface for functional movements in hemiplegics patients

We describe the new multi-contact electrode-array for surface electrical stimulation, and the corresponding interface device that allows on-line selection of the conductive fields during the application of the system. This new device has a specific value for therapeutic applications of electrical stimulation since it allows effective generation of desired functional movements. The user-friendly interface also allows patients at home to select the optimal electrode array; thereby, to receive therapies out of the clinical environment. The electrode was tested in three post-stroke hemiplegics patients. The pilot experiments showed that system works sufficiently good for control of fingers during grasp and release functions without the interference of the wrist movement. The use of electrode is also envisioned for many other applications (foot-drop fitness, shoulder subluxation, etc)

The Role of Ovalbumin in Manganese Homeostasis during Chick Embryogenesis: An EPR Spectroscopic Study

Ovalbumin (OVA), a protein vital for chick embryo nutrition, hydration, and antimicrobial protection, together with other egg-white proteins, migrates to the amniotic fluid and is orally absorbed by the embryo during embryogenesis. Recently, it has been shown that for optimal eggshell quality, the hen diet can be supplemented with manganese. Although essential for embryonic development, manganese in excess causes neurotoxicity. This study investigates whether OVA may be involved in the regulation of manganese levels. The binding of Mn(II) to OVA was investigated using electron paramagnetic resonance (EPR) spectroscopy. The results show that OVA binds a maximum of two Mn(II) ions, one with slightly weaker affinity, even in a 10-fold excess, suggesting it may have a protective role from Mn(II) overload. It seems that the binding of Mn(II), or the presence of excess Mn(II), does not affect OVA’s tertiary structure, as evidenced from fluorescence and UV/vis measurements. Comparative analysis with bovine and human serum albumins revealed that they exhibit higher affinities for Mn(II) than OVA, most likely due to their essentially different physiological roles. These findings suggest that OVA does not play a role in the transport and storage of manganese; however, it may be involved in embryo protection from manganese-induced toxicity

Glucosomes: Magnetically induced controlled release of glucose modified liposomes

Novel methods of cancer therapy are constantly being investigated since the current approach heavily relies on the use of non-specific and toxic chemotherapy agents. Ideally, a drug used for cancer therapy would specifically target tumor sites or rather bind specifically with cancer cells. The way to achieve this is by targeting cancer cell specific receptors or receptors present in abnormally high counts at the surface. Rapid proliferation of cancer cells is fueled by large amounts of energy that is in turn produced by abnormal glucose uptake. Because of this high energy/glucose demand, cancer cells exhibit an abnormally high glucose receptor (GLUTs) count on their surface, compared to normal, healthy cells. We have utilized this glucose dependency to create glucose modified liposomes (Glucosomes) that are specifically bound by cancer cells. Glucosomes can be used to transport different substances, either hydrophilic or hydrophobic, and can therefore deliver any type of drug to cancer cells, increasing its efficiency. Another important aspect to consider is the controlled release of the drug being transported in order to maximize therapeutic efficiency. Controlled release can be achieved by utilizing different internal or external influences. In our study, we have used standard Fe3O4 magnetic nanoparticles to load glucosomes and induce their controlled opening via an external magnetic field. By applying an external magnetic field, the magnetic nanoparticles start heating up and transferring this thermal energy to the surrounding lipid bilayer, causing its perturbation and opening of the glucosome. Our study has found that controlled release can be achieved with high efficiency while the chemical stability of the Fe3O4 nanoparticles stays practically intact. Using EPR spectroscopy, we have shown that Fe3O4 nanoparticles remain trapped within the lipid bilayer and are essentially protected from oxidation that would diminish their magnetic properties. Since magnetic Fe3O4 nanoparticles are lodged well within the lipid bilayer no thermal damage can be caused to the drug being transported within the glucosome bilayer, making this a viable controlled release cancer targeting drug delivery system.Twentieth Young Researchers’ Conference - Materials Science and Engineering: Program and the Book of Abstracts; November 30 – December 2, 2022, Belgrade, Serbi

Supplementary data for article: Božić, B.; Korać, J.; Stanković, D. M.; Stanić, M.; Popović-Bijelić, A.; Bogdanović Pristov, J.; Spasojević, I.; Bajčetić, M. Mechanisms of Redox Interactions of Bilirubin with Copper and the Effects of Penicillamine. Chemico-Biological Interactions 2017, 278, 129–134. https://doi.org/10.1016/j.cbi.2017.10.022

Supplementary material for: [https://doi.org/10.1016/j.cbi.2017.10.022]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2570]Related to accepted version: [http://cherry.chem.bg.ac.rs/handle/123456789/3105

Supplementary data for the article: Božić, B.; Korać, J.; Stanković, D. M.; Stanić, M.; Romanović, M.; Pristov, J. B.; Spasić, S.; Popović-Bijelić, A.; Spasojević, I.; Bajčetić, M. Coordination and Redox Interactions of β-Lactam Antibiotics with Cu2+ in Physiological Settings and the Impact on Antibacterial Activity. Free Radical Biology and Medicine 2018, 129, 279–285. https://doi.org/10.1016/j.freeradbiomed.2018.09.038

Supplementary material for: [https://doi.org/10.1016/j.freeradbiomed.2018.09.038]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/349]Related to accepted version: [http://cherry.chem.bg.ac.rs/handle/123456789/2940

Supplementary data for article: Božić, B.; Korać, J.; Stanković, D. M.; Stanić, M.; Popović-Bijelić, A.; Bogdanović Pristov, J.; Spasojević, I.; Bajčetić, M. Mechanisms of Redox Interactions of Bilirubin with Copper and the Effects of Penicillamine. Chemico-Biological Interactions 2017, 278, 129–134. https://doi.org/10.1016/j.cbi.2017.10.022

Supplementary material for: [https://doi.org/10.1016/j.cbi.2017.10.022]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2570]Related to accepted version: [http://cherry.chem.bg.ac.rs/handle/123456789/3105

Supplementary data for the article: Božić, B.; Korać, J.; Stanković, D. M.; Stanić, M.; Romanović, M.; Pristov, J. B.; Spasić, S.; Popović-Bijelić, A.; Spasojević, I.; Bajčetić, M. Coordination and Redox Interactions of β-Lactam Antibiotics with Cu2+ in Physiological Settings and the Impact on Antibacterial Activity. Free Radical Biology and Medicine 2018, 129, 279–285. https://doi.org/10.1016/j.freeradbiomed.2018.09.038

Supplementary material for: [https://doi.org/10.1016/j.freeradbiomed.2018.09.038]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/349]Related to accepted version: [http://cherry.chem.bg.ac.rs/handle/123456789/2940