The interprofessional organizations - from the need of establishment to the need of development and affirmation

Through the present study it aims to represent the actual image of the interprofessional organizations on product chain in Romania, reflecting briefly the establishment and development issues. In the context of the reorganization of entire agrifood production system, in dealing with future crises, whatever is their nature, it’s needed to seriously manage the organizational issues and to present them as a useful tool for increasing the access to new markets for producers and to offer local products quality to consumers in Romania both. Using the documentation method it was obtained an overview of the relationship between the need of affirmation and the real need to convert the interprofessional organizations in Romania in functional structures. The Ministry of Agriculture and Rural Development recognized till now only five IPO but the analysis and the directly involved interprofessional organizations opinions exchanges, it was emerged some primordial issues in order to improve the legislation of interprofessional organizations on product chain. The interprofessional organizations on product chain are meant, almost in ingrate way, to be interposed between the pressing help need in the development of intra-community trade relationships and the stiffness required by the legislation

Reducing the Sludge Quantity Produced from Used Water Purification – A Source of Profit

This paper has its origins in the analysis of a real situation met in the Water Purification Plant from Cluj-Napoca city. The produced dehydrated sludge is about 70 - 80 tonnes / 24 hours, with a density that allows for an equivalence of 1 tonne to 1 m³, 80,000 Kg or 80 m³, i.e. a huge amount and an impressive volume of material. This paper does not propose solutions to valorise or store the huge quantity of sludge, though the present solutions are quite inefficient. The stress is placed upon the sludge production sources, its transportation to the purification plants, the collection of the sludge in the purification plants and the dehydration methods that can be used. The analysis of these stages aims at identifying several measures that could lead to diminishing the amount of sludge produced after the purification of used and dehydrated water in the purification plants without affecting the quality of purified water. The measures identified are to be presented as measures that can be applied in the spirit of sustainable and efficient development. The business feature is given by the savings brought by the mitigation of the sludge amount to be stored and paid for in the ecological storage places in remote areas

Glioblastoma and glioblastoma stem-like cancer cells radiosensitization to X-ray and carbon ions using ATM and ATR inhibitors

International audienceGlioblastoma multiform (GBM) represents the deadliest brain cancer, being extremely chemo- and radioresistant. To overcome the resistance to radiotherapy, the use of DNA repair inhibitors was explored for photons and particle irradiation. The treatment with ATM (AZD1390, 10nM) and ATR (VE822, 100nM) DNA repair inhibitors in combination with X-ray and carbon ions was investigated in several commercial human GBM cell lines: U251, U87, U118, T98G and two stem-like cell lines: R633 (p53 mutated) and TG-1 (ATM missense mutation), isolated from neurosurgical biopsy of human GBM. The cells were treated with inhibitors before exposure to X-ray or carbon ion beam (28 and 73KeV/um2). Proliferation index, LDH release and senescence induction were evaluated 5 days post-irradiation. In addition, clonogenic survival was measured inU251 cells. The cell lines range from most radiosensitive to most radioresistant as follows: U87, TG-1, R633, T98G, U251, U118. ATMi induced a higher sensitization than ATRi in all cell lines, as measured by poliferation index after X-ray irradiation. The highest sensitization was induced in U87, U118 and U251 cells and the lowest in the stem-like cells, were ATRi had almost no effect. LDH release is not induced by inhibitors alone, but it is detected following irradiation alone and in combination with inhibitors. Stem-like cells presented more LDH release suggesting higher cellular death. Radiation alone and combination with DNA damage inhibitors induced senescence in the most radiosensitive cell lines (U118, U87) and in both stem-like cells. Clonogenic survival showed lower survival following carbon ions then X-ray, and moreover, a lower survival at higher LET beam. In conclusion, the proposed inhibitors are a promising tool for glioblastoma treatment in combination with radiotherapy. However, individual genetic and epigenetic background likely affects radiosensitivity and treatment response of tumor cell and can modulate the therapeutic outcome

Glioblastoma and glioblastoma stem-like cancer cells radiosensitization to X-ray and carbon ions using ATM and ATR inhibitors

International audienceGlioblastoma multiform (GBM) represents the deadliest brain cancer, being extremely chemo- and radioresistant. To overcome the resistance to radiotherapy, the use of DNA repair inhibitors was explored for photons and particle irradiation. The treatment with ATM (AZD1390, 10nM) and ATR (VE822, 100nM) DNA repair inhibitors in combination with X-ray and carbon ions was investigated in several commercial human GBM cell lines: U251, U87, U118, T98G and two stem-like cell lines: R633 (p53 mutated) and TG-1 (ATM missense mutation), isolated from neurosurgical biopsy of human GBM. The cells were treated with inhibitors before exposure to X-ray or carbon ion beam (28 and 73KeV/um2). Proliferation index, LDH release and senescence induction were evaluated 5 days post-irradiation. In addition, clonogenic survival was measured inU251 cells. The cell lines range from most radiosensitive to most radioresistant as follows: U87, TG-1, R633, T98G, U251, U118. ATMi induced a higher sensitization than ATRi in all cell lines, as measured by poliferation index after X-ray irradiation. The highest sensitization was induced in U87, U118 and U251 cells and the lowest in the stem-like cells, were ATRi had almost no effect. LDH release is not induced by inhibitors alone, but it is detected following irradiation alone and in combination with inhibitors. Stem-like cells presented more LDH release suggesting higher cellular death. Radiation alone and combination with DNA damage inhibitors induced senescence in the most radiosensitive cell lines (U118, U87) and in both stem-like cells. Clonogenic survival showed lower survival following carbon ions then X-ray, and moreover, a lower survival at higher LET beam. In conclusion, the proposed inhibitors are a promising tool for glioblastoma treatment in combination with radiotherapy. However, individual genetic and epigenetic background likely affects radiosensitivity and treatment response of tumor cell and can modulate the therapeutic outcome

Ethical implications of developing RNA-based therapies for cardiovascular disorders

The awareness concerning RNA-based therapies was boosted significantly after the successful development of COVID-19 vaccines. However, they can potentially lead to significant advances in other areas of medicine, such as oncology or chronic diseases. In recent years, there has been an exponential increase in the number of RNA-based therapies that were evaluated as potential treatments for cardiovascular disorders. One of the areas that was not explicitly assessed about these therapies is represented by their overall ethical framework. Some studies evaluate ethical issues of RNA-based treatments in general or targeting specific disorders (especially neurodegenerative) or interventions for developing RNA-based vaccines. Much less information is available regarding the ethical issues associated with developing these therapeutic strategies for cardiovascular disorders, which is the main aim of this study. We will focus our analysis on three main topics: risk-benefit analysis (including the management of public awareness about these technologies), and justice (in both research and clinical medicine)