ARROZ PRETO (Oryza sativa L.) UMA EXCELENTE FONTE DE ANOTOCIANINAS

O arroz preto, nutricionalmente superior ao arroz branco por possuir teores mais elevados de proteínas e fibras tem sido alvo do interesse gastronômico nacional e internacional devido à presença de pigmentos, sabor e aroma diferenciados.  O pigmento presente no pericarpo do arroz preto pertence à classe das antocianinas, substâncias que têm sido pesquisadas devido as suas contribuições na redução do risco de doenças crônicas. Neste trabalho tais substâncias foram investigadas no pericarpo da cultivar de arroz preto IAC 600, desenvolvida pelo Instituto Agronômico de Campinas (IAC) e adaptada às condições climáticas do Brasil. Para isso, foi utilizado Cromatógrafo Líquido de Ultra eficiência (CLUE) acoplado ao Espectrômetro de Massas de Alta Resolução (ESI-Q-TOF-EM). Foram identificadas cinco antocianinas: cianidina-3,5-O-diglicosídeo, cianidina-3-O-diglicosídeo, cianidina-3-O-glicosídeo (majoritária), cianidina-3-O-rutenosídeo e peonidina-3-O-glicosídeo

Analytical standards production for the analysis of pomegranate anthocyanins by HPLC

Pomegranate (Punica granatum L.) is a fruit with a long medicinal history, especially due to its phenolic compounds content, such as the anthocyanins, which are reported as one of the most important natural antioxidants. The analysis of the anthocyanins by high performance liquid chromatography (HPLC) can be considered as an important tool to evaluate the quality of pomegranate juice. For research laboratories the major challenge in using HPLC for quantitative analyses is the acquisition of high purity analytical standards, since these are expensive and in some cases not even commercially available. The aim of this study was to obtain analytical standards for the qualitative and quantitative analysis of the anthocyanins from pomegranate. Five vegetable matrices (pomegranate flower, jambolan, jabuticaba, blackberry and strawberry fruits) were used to isolate each of the six anthocyanins present in pomegranate fruit, using an analytical HPLC scale with non-destructive detection, it being possible to subsequently use them as analytical standards. Furthermore, their identities were confirmed by high resolution mass spectrometry. The proposed procedure showed that it is possible to obtain analytical standards of anthocyanins with a high purity grade (98.0 to 99.9%) from natural sources, which was proved to be an economic strategy for the production of standards by laboratories according to their research requirements

Acylated anthocyanins from organic purple-fleshed sweet potato (Ipomoea batatas (L.) Lam) produced in Brazil

Acylated anthocyanins from a purple-fleshed sweet potato (PFSP), obtained by organic cultivation in Brazil, were characterized after separation by a high performance liquid chromatography-diode array detector (HPLC-PDA). These anthocyanins were manually collected at the detector output, concentrated and injected into a high resolution mass spectrometer (ESIQTOF-MS2). Twenty-two acylated anthocyanins were detected. Among them, sixteen had been reported in the literature and six, derived from peonidin were reported for the first time in sweet potato roots in this study. These compounds showed molecular ions with accurate mass/charge ratios (m/z) of 909.2081, 961.3010, 961.2571, 963.3345, 1123.2932 and 1179.3862. Although anthocyanins in PFSP have already been extensively studied, the variety studied in this work is probably genetically different from all varieties and cultivars already researched, which would explain why these anthocyanins have not been observed in the previously studied varieties