Antibiotic Resistance in Manure- Amended Agricultural Soils

Manure application to agricultural land benefits soil health and agronomic yields. However, as antibiotic resistance becomes a more serious threat to public health, there is concern that antibiotic resistance originating from livestock manure could impact human health through contamination of the environment or food. This study sought to quantify this risk by monitoring concentrations of antibiotic resistance bacteria and genes in fallow soil during the period of October through April, representing fall manure application through spring planting. Resistance to three common antibiotics— tylosin, azithromycin and tetracycline— was monitored following application of fresh, stockpiled, or composted beef feedlot manure, or inorganic fertilizer. Overall, concentrations of all monitored resistant bacteria were below the detection limit for enumeration. Results indicate that while all the manure treatments increased at least one measure of antibiotic resistance during the sampling period, by the final sampling day antibiotic resistance prevalence and concentrations in manured plots were not significantly different from soil receiving no fertilizer treatments

MendelianRandomization v0.5.0: updates to an R package for performing Mendelian randomization analyses using summarized data.

The MendelianRandomization package is a software package written for the R software environment that implements methods for Mendelian randomization based on summarized data. In this manuscript, we describe functions that have been added to the package or updated in recent years. These features can be divided into four categories: robust methods for Mendelian randomization, methods for multivariable Mendelian randomization, functions for data visualization, and the ability to load data into the package seamlessly from the PhenoScanner web-resource. We provide examples of the graphical output produced by the data visualization commands, as well as syntax for obtaining suitable data and performing a Mendelian randomization analysis in a single line of code

Hedgerow rejuvenation management affects invertebrate communities through changes to habitat structure

Hedgerows are an important semi-natural habitat for invertebrates and other wildlife within agricultural landscapes. Hedgerow quality can be greatly affected either by over- or under-management. Neglect of hedgerows is an increasingly important issue as traditional management techniques such as hedgelaying become economically unviable. In the UK, funding for hedge management is available under agri-environment schemes but relatively little is known about how this impacts on wider biodiversity. We used a randomised block experiment to investigate how habitat structural change, arising from a range of techniques to rejuvenate hedgerows (including more economic/mechanised alternatives to traditional hedgelaying), affected invertebrate abundance and diversity. We combined digital image analysis with estimates of foliage biomass and quality to show which aspects of hedge structure were most affected by the rejuvenation treatments. All investigated aspects of habitat structure varied considerably with management type, though the abundance of herbivores and predators was affected primarily by foliage density. Detritivore abundance was most strongly correlated with variation in hedge gap size. The results suggest that habitat structure is an important organising force in invertebrate community interactions and that management technique may affect trophic groups differently. Specifically we find that alternative methods of hedgerow rejuvenation could support abundances of invertebrates comparable or even higher than traditional hedgelaying, with positive implications for the restoration of a larger area of hedgerow habitat on a limited budget

Experimental evidence for optimal hedgerow cutting regimes for brown hairstreak butterflies

1. The Brown hairstreak butterfly has declined in range and abundance over the past 50 years, leading to designated conservation status in several European countries including England and Wales. The Brown hairstreak's decline has been linked to changes in hedgerow management, based on mortality of eggs over winter and female oviposition preferences. 2. We assessed Brown hairstreak egg abundance in late winter over 4 years in response to hedgerow management treatments to manipulate the frequency, timing, and the intensity of trimming (reduced intensity resulting in an annual increase of approximately 10 cm in hedge height and width), using a field experiment with a randomised block design. 3. Hedgerow plots cut every year to a standard height and width had the lowest Brown hairstreak egg abundance; this is the most common hedgerow management outside agri-environment schemes (AES). Cutting hedgerow plots at a reduced intensity nearly doubled the number of surviving eggs in late winter. Plots cut at a reduced frequency in autumn (once every 3 years), which forms part of current English AES, had 1.3 times more eggs than those cut annually. 4. Current AES management prescriptions are likely to benefit the Brown hairstreak, but its requirements need to be balanced with those of other taxa in relation to the timing of hedgerow cutting. Cutting hedges at a reduced intensity has previously been shown to benefit the wider Lepidoptera community as well as Brown hairstreak butterflies. Reduced intensity cutting does not currently form part of AES hedgerow prescriptions, but could be considered for inclusion in future schemes

Automation-Induced Complacency Potential: Development and Validation of a New Scale

Complacency, or sub-optimal monitoring of automation performance, has been cited as a contributing factor in numerous major transportation and medical incidents. Researchers are working to identify individual differences that correlate with complacency as one strategy for preventing complacency-related accidents. Automation-induced complacency potential is an individual difference reflecting a general tendency to be complacent across a wide variety of situations which is similar to, but distinct from trust. Accurately assessing complacency potential may improve our ability to predict and prevent complacency in safety-critical occupations. Much past research has employed an existing measure of complacency potential. However, in the 25 years since that scale was published, our conceptual understanding of complacency itself has evolved, and we propose that an updated scale of complacency potential is needed. The goal of the present study was to develop, and provide initial validation evidence for, a new measure of automation-induced complacency potential that parallels the current conceptualization of complacency. In a sample of 475 online respondents, we tested 10 new items and found that they clustered into two separate scales: Alleviating Workload (which focuses on attitudes about the use of automation to ease workloads) and Monitoring (which focuses on attitudes toward monitoring of automation). Alleviating workload correlated moderately with the existing complacency potential rating scale, while monitoring did not. Further, both the alleviating workload and monitoring scales showed discriminant validity from the previous complacency potential scale and from similar constructs, such as propensity to trust. In an initial examination of criterion-related validity, only the monitoring-focused scale had a significant relationship with hypothetical complacency (r = -0.42, p < 0.01), and it had significant incremental validity over and above all other individual difference measures in the study. These results suggest that our new monitoring-related items have potential for use as a measure of automation-induced complacency potential and, compared with similar scales, this new measure may have unique value

Research priorities for mitochondrial disorders: Current landscape and patient and professional views

Primary mitochondrial disorders encompass a wide range of clinical presentations and a spectrum of severity. They currently lack effective disease-modifying therapies and have a high mortality and morbidity rate. It is therefore essential to know that competitively-funded research designed by academics meets core needs of people with mitochondrial disorders and their clinicians. The Priority Setting Partnerships are an established collaborative methodology that brings patients, carers and families, charity representatives and clinicians together to try to establish the most pressing and unanswered research priorities for a particular disease. We developed a web-based questionnaire, requesting all patients affected by primary mitochondrial disease, their carers, and clinicians to pose their research questions. This yielded 709 questions from 147 participants. These were grouped into overarching themes including basic biology, causation, health services, clinical management, social impacts, prognosis, prevention, symptoms, treatment, and psychological impact. Following the removal of 'answered questions' the process resulted in a list of 42 discrete, answerable questions. This was further refined by web-based ranking by the community to 24 questions. These were debated at a face-to-face workshop attended by a diverse range of patients, carers, charity representatives and clinicians to create a definitive 'Top Ten of unanswered research questions for primary mitochondrial disorders'. These Top Ten questions related to understanding biological processes, including triggers of disease onset, mechanisms underlying progression and reasons for differential symptoms between individuals with identical genetic mutations; new treatments; biomarker discovery; psychological support; and optimal management of stroke-like episodes and fatigue

Re-structuring hedges: rejuvenation management can improve the long term quality of hedgerow habitats for wildlife in the UK

Hedgerows provide key wildlife habitat in intensive agricultural landscapes, but are declining in length and structural condition due to a lack of rejuvenation management, neglect and over-frequent trimming with mechanised flails. Here, we test cheaper, alternative methods to traditional hedge laying methods using a multi-site manipulative field experiment. In the first quantitative test of new approaches to hedge rejuvenation management, hedge regrowth, structure, berry provision for over-wintering wildlife and cost of rejuvenation were assessed in response to five methods, for three years following rejuvenation. Three ‘laying’ methods and coppicing were effective at improving hedgerow condition by stimulating basal regrowth, thus increasing the density of woody material at the base and reducing gap size. The pros and cons of coppicing are discussed in relation to its impact on different wildlife groups, and it is recommended in limited circumstances. Differences between the three ‘laying’ methods reduced over time, so a cheaper conservation hedging method is recommended as an alternative to traditional hedge laying. This new approach to hedge management offers the potential to restore twice the length of hedgerow currently rejuvenated under agri-environment schemes

Brief Report: Correlates of Quality of Life-related Outcomes in Breast Cancer Patients Participating in the Pathfinders Pilot Study

In a pilot study, participation in the Pathfinders program was associated with reductions in distress and despair and improvements in quality of life (QOL) among advanced breast cancer patients. This paper explores the relationship between psychosocial resources invoked through the Pathfinders intervention and outcomes

Phase 2 pilot study of Pathfinders: a psychosocial intervention for cancer patients

Pathfinders is a multi-faceted psychosocial care program for cancer patients; it was developed in community oncology and adapted to the academic oncology setting. This prospective, single-arm, phase 2 pilot study examined the acceptability and feasibility of Pathfinders for women with metastatic breast cancer

Little and late: how reduced hedgerow cutting can benefit Lepidoptera

Hedgerows are a key semi-natural habitat for biodiversity in intensive agricultural landscapes across northern Europe and support a large invertebrate fauna. Management can have large effects on the value of hedgerows as a wildlife habitat, thus sensitive management is incentivised through agri-environment schemes (AES). We tested how current and potential future AES hedge management regimes affected the diversity and abundance of Lepidoptera species that utilise the hedge as a breeding resource, using a long term, multi-site, manipulative field experiment. Hedgerow management in some current AES options (reduced trimming frequency and cutting in winter) increased Lepidoptera abundance and the diversity of components of the Lepidoptera community linked with specific lifecycle traits. However, the most frequently applied hedgerow AES option currently applied in the UK (cutting once every 2 years in autumn) did not benefit Lepidoptera compared to standard hedgerow management outside AES (annual trimming in autumn). Decreasing the intensity of hedgerow trimming improves the diversity of the whole Lepidoptera assemblage, and should be considered as part of biodiversity conservation in farmed landscapes