Walking For Health: A Community Education and Physical Activity Initiative

Background/Introduction: Vermont was ranked the nation’s healthiest state, according to 2007 America’s Health Rankings. However obesity, currently the second most common cause of death among VT adults, is becoming so common it may replace cigarette smoking as the number one risk factor for death. In fact obesity affects 21% of adults in VT, most commonly low income adults. Obesity is a risk factor for high cholesterol, high blood pressure, heart diseases, and diabetes. Diet quality and physical activity are important factors in preventing obesity. 42% of Vermont adults are below the recommended level of physical activity. Greater knowledge about nutrition correlates with improved diet quality and greater physical activity. A successful educational strategy on physical activity and nutrition promotes group activities and adapts for cultural relevance.https://scholarworks.uvm.edu/comphp_gallery/1013/thumbnail.jp

Detection of Intra-Tumor Self Antigen Recognition during Melanoma Tumor Progression in Mice Using Advanced Multimode Confocal/Two Photon Microscope

Determining how tumor immunity is regulated requires understanding the extent to which the anti-tumor immune response “functions” in vivo without therapeutic intervention. To better understand this question, we developed advanced multimodal reflectance confocal/two photon fluorescence intra-vital imaging techniques to use in combination with traditional ex vivo analysis of tumor specific T cells. By transferring small numbers of melanoma-specific CD8+ T cells (Pmel-1), in an attempt to mimic physiologic conditions, we found that B16 tumor growth alone was sufficient to induce naive Pmel-1 T cell proliferation and acquisition of effector phenotype. Tumor -primed Pmel-1 T cells, are capable of killing target cells in the periphery and secrete IFNγ, but are unable to mediate tumor regression. Within the tumor, Pmel-1 T cells have highly confined mobility, displaying long term interactions with tumor cells. In contrast, adoptively transferred non tumor-specific OT-I T cells show neither confined mobility, nor long term interaction with B16 tumor cells, suggesting that intra-tumor recognition of cognate self antigen by Pmel-1 T cells occurs during tumor growth. Together, these data indicate that lack of anti-tumor efficacy is not solely due to ignorance of self antigen in the tumor microenvironment but rather to active immunosuppressive influences preventing a protective immune response

Old and recent processes in a warm and humid desert hypogene cave: ‘A’rak Na‘asane, Israel

Recent environmental processes are studied in ʻA’rak Naʻasane Cave at the northern Judean Desert, Israel. The outer zone of the cave is heavily influenced by the outside environment through a large entrance, facilitating entry of air flow, fauna and humans, with minor cave-forming modifications. Conversely, the inner cave sustains humid and warm conditions, favoring modifications by condensation corrosion of convective air flow, associated with deposition of popcorn speleothems at the lower parts of dissolution pockets. The warm humid air of the inner cave may be associated with an underlying thermal water table. Active condensation corrosion is decreasing, possibly because of gradual change in the cave microclimate, associated with falling water table and ventilation. Increasing connection with the surface is indicated by high collapse domes, rare flood invasion, and a large Trident Leaf-nosed bat community which spends the winter within the innermost parts of the cave. Bat guano supports bedrock corrosion and a rich invertebrate fauna, but humans preferred the outer parts of the cave, particularly for refuge during the second Jewish revolt against the Romans. Rare occasions of ancient human entry into the inner cave support this scenario by the small number of artifacts compared with the outer cave. Enigmatic small cairns in the largest inner hall were probably erected during the Intermediate Bronze Age

Old and recent processes in a warm and humid desert hypogene cave: ‘A’rak Na‘asane, Israel

Recent environmental processes are studied in ʻA’rak Naʻasane Cave at the northern Judean Desert, Israel. The outer zone of the cave is heavily influenced by the outside environment through a large entrance, facilitating entry of air flow, fauna and humans, with minor cave-forming modifications. Conversely, the inner cave sustains humid and warm conditions, favoring modifications by condensation corrosion of convective air flow, associated with deposition of popcorn speleothems at the lower parts of dissolution pockets. The warm humid air of the inner cave may be associated with an underlying thermal water table. Active condensation corrosion is decreasing, possibly because of gradual change in the cave microclimate, associated with falling water table and ventilation. Increasing connection with the surface is indicated by high collapse domes, rare flood invasion, and a large Trident Leaf-nosed bat community which spends the winter within the innermost parts of the cave. Bat guano supports bedrock corrosion and a rich invertebrate fauna, but humans preferred the outer parts of the cave, particularly for refuge during the second Jewish revolt against the Romans. Rare occasions of ancient human entry into the inner cave support this scenario by the small number of artifacts compared with the outer cave. Enigmatic small cairns in the largest inner hall were probably erected during the Intermediate Bronze Age

Magnetic Targeting of Growth Factors Using Iron Oxide Nanoparticles

Growth factors play an important role in nerve regeneration and repair. An attractive drug delivery strategy, termed “magnetic targeting”, aims to enhance therapeutic efficiency by directing magnetic drug carriers specifically to selected cell populations that are suitable for the nervous tissues. Here, we covalently conjugated nerve growth factor to iron oxide nanoparticles (NGF-MNPs) and used controlled magnetic fields to deliver the NGF–MNP complexes to target sites. In order to actuate the magnetic fields a modular magnetic device was designed and fabricated. PC12 cells that were plated homogenously in culture were differentiated selectively only in targeted sites out of the entire dish, restricted to areas above the magnetic “hot spots”. To examine the ability to guide the NGF-MNPs towards specific targets in vivo, we examined two model systems. First, we injected and directed magnetic carriers within the sciatic nerve. Second, we injected the MNPs intravenously and showed a significant accumulation of MNPs in mouse retina while using an external magnet that was placed next to one of the eyes. We propose a novel approach to deliver drugs selectively to injured sites, thus, to promote an effective repair with minimal systemic side effects, overcoming current challenges in regenerative therapeutics