Do All Opioid Drugs Share the Same Immunomodulatory Properties? A Review From Animal and Human Studies

Suppression of the immune system has been constantly reported in the last years as a classical side effect of opioid drugs. Most of the studies on the immunological properties of opioids refer to morphine. Although morphine remains the "reference molecule," other semisynthetic and synthetic opioids are frequently used in the clinical practice. The primary objective of this review is to analyze the available literature on the immunomodulating properties of opioid drugs different from morphine in preclinical models and in the human. A search strategy was conducted in PubMed, Embase, and the Cochrane databases using the terms "immunosuppression," "immune system," "opioids," "Natural killer cells," "cytokines," and "lymphocytes." The results achieved concerning the effects of fentanyl, methadone, oxycodone, buprenorphine, remifentanil, tramadol, and tapentadol on immune responses in animal studies, in healthy volunteers and in patients are reported. With some limitations due to the different methods used to measure immune system parameters, the large range of opioid doses and the relatively scarce number of participants in the available studies, we conclude that it is not correct to generalize immunosuppression as a common side effect of all opioid molecules

A novel technology for disinfecting surfaces infested with Candida auris: the UVC chip

Background The fungal pathogen Candida Auris is increasingly associated with multidrug-resistant infections that are highly expensive for the Health Care System. The spreading of this pathogen can occur, among others, through contact with infected surfaces or medical instruments. This study evaluated the efficacy of a novel UVC chip, novel alternative to UVC LEDs and lamps, in inactivating Candida auris strain. Methods This experimental study was carried out between July and September 2020 at the University of Siena. Candida auris (ATCC 12372) at two known concentrations (1.5X107 and 1.5x106 CFU/ml) at a fixed distance (7,5 cm) from the chip (5.1mW radiant power) was tested, in triplicates, with three exposure times (5, 10 and 15 minutes). Potato Dextrose Agar (PDA) plates without the plate lid and containing Candida auris were exposed to UVC light. Subsequently, the plates were incubated at 36 °C for 48 h. Log reduction between treated and positive control (not exposed to UVC light) samples was calculated. Results At 15 minutes, we had the highest inactivation result, mean 4.43 log10, starting from a 1.5x106 CFU/mL concentration. At a higher concentration, 1.5X107 CFU/mL, the reduction had a mean of 3.51 log10. Conclusions The results of the experiments showed a significant microbial reduction in relation to the exposure time. The highest level of reduction was reached after 15 minutes of exposure. UVC chip had a relevant biocidal effect on Candida auris and may represent a valuable tool in the prevention of infections caused by this pathogen, which is becoming increasingly prevalent and persistent globally. Key messages • The use of UVC Chip decreases surface contamination. • New technology against healthcare-associated infections

Wind of change: Better air for microbial environmental control

Background: The COVID19 epidemic highlighted the importance of air in the transmission of pathogens. Air disinfection is one of the key points to reduce the risk of transmission both in the health sector and in public, civil and industrial environments. All bacteria and viruses tested to date can be inactivated by UV-C rays. Laboratory tested UV-C systems are increasingly popular and proposed as effective technologies for air purification; few studies have evaluated their performance in populated indoor environments. The aim of this investigation was to evaluate the effectiveness of a UV-C disinfection system for air in a real working context. Methods: This experimental study was conducted between December 2020 and February 2021 in an office of the Department of Molecular and Developmental Medicine of the University of Siena, Italy. A pre-final version air purifier (Cleaning Air T12), capable of treating 210 m3/h of air, was first tested for its ability to filter particulates and reduce microbial air contamination in the absence of people. Subsequently, the experiments were conducted in the presence of 3–5 subjects who worked for several hours in an office. During the tests, microbiological samples of air were collected in real time, switching the system on and off periodically. Air samples were collected and incubated on Petri dishes at 36 ◦C and 22 ◦C. Statistical analysis was performed with Stata 16 software assuming a significance level of 95%. An interpolating model was identified to describe the dynamics of contamination reduction when the device operates. Results: Preliminary tests showed a significant 62.5% reduction in Colony-Forming Units (CFUs) with 36 ◦C incubation. Reductions in the particulate component were also observed. In the main test, comparison of CFU data, between the device-on phase (90 min) and the subsequent device-off phase (60 min), showed statistically significant increase (p = 0.001) of environmental contamination passing from a mean of 86.6 (65.8–107.4) to 171.1 (143.9–198.3) CFU/m3, that is a rise of about 100%. The interpolating model exhibited a good fit of CFU reduction trend with the device on. Conclusions: The system, which mainly uses UV-C lamps for disinfection, was able to significantly reduce envi- ronmental and human contamination in real time. Experimental tests have shown that as soon as the device is switched off, after at least half an hour of operation, the healthiness of the air decreases drastically within 10 minutes, bringing the airborne microbial contamination (induced by the presence of operators in the environ- ment) to levels even higher than 150% of the last value with the device on. Re-engineering strategies for system improvement were also discusse

Stylish UV-C lamp for disinfecting household objects

In this study, UV-C device proved to be a valuable tool for disinfecting household items and enhancing safety for everyday healt

Low-Velocity Impacts on a Polymeric Foam for the Passive Safety Improvement of Sports Fields: Meshless Approach and Experimental Validation

Over the past few years, foam materials have been increasingly used in the passive safety of sport fields, to mitigate the risk of crash injury. Currently, the passive safety certification process of these materials represents an expensive and time-consuming task, since a considerable number of impact tests on material samples have to be carried out by an ad hoc testing apparatus. To overcome this difficulty and speed up the design process of new protective devices, a virtual model for the low-velocity impact behaviour of foam protective mats is needed. In this study a modelling approach based on the mesh-free Element Galerkin method was developed to investigate the impact behaviour of ethylene-vinyl acetate (EVA) foam protective mats. The main advantage of this novel technique is that the difficulties related to the computational mesh distortion and caused by the large deformation of the foam material are avoided and a good accuracy is achieved at a relatively low computational cost. The numerical model was validated statistically by comparing numerical and experimental acceleration data acquired during a series of impact events on EVA foam mats of various thicknesses. The findings of this study are useful for the design and improvement of foam protective devices and allow for optimizing sports fields’ facilities by reducing head injury risk by a reliable computational method

Safer school with near-UV technology: novel applications for environmental hygiene

Systems capable of disinfecting air and surfaces could reduce the risk of infectious diseases transmission. Aim: to evalu-ate the effectiveness of near-UV LED ceiling lamps, with a wavelength of 405 nm, in improving environmental hygiene. Between November and December 2020, we conducted an experimental study having a pre-post design in a kindergarten room in Siena where 4 ceiling lamps with 405 nm LED technology were installed. Twice per day, sampling was performed before (T0) and after treatment with near-UV (T1). We used between 8 and 12 pairs of contact plates to sample at various random spots each day. Air samplings were also performed. The plates were incubated at 22 and 36 degrees C. Significance was set at 95% (p < 0.05). The mean level of Colony Forming Unit (CFU) at T(0) was 249 (95% CI 193.1 - 305.0) at 36 degrees C and 535.2 (374.3 - 696.1) at 22 degrees C. The reduction was significant at T(1): by 65% at 36 degrees C and, 72% at 22 degrees C. Also, for air contamination: 95.3% (98.4 & mdash;92.3). A dose threshold of about 5 J/cm2 was identified to have an 80% CFU abatement and remains nearly constant. The advantage of being able to use this technology in the presence of people is very important in the context of controlling environmental contamination. © 2023, The Author(s)

The antagonism of the prokineticin system counteracts bortezomib induced side effects: Focus on mood alterations

The development of neuropathy and of mood alterations is frequent after chemotherapy. These complications, independent from the antitumoral mechanism, are interconnected due to an overlapping in their processing pathways and a common neuroinflammatory condition. This study aims to verify whether in mice the treatment with the proteasome inhibitor bortezomib (BTZ), at a protocol capable of inducing painful neuropathy, is associated with anxiety, depression and supraspinal neuroinflammation. We also verify if the therapeutic treatment with the antagonist of the prokineticin (PK) system PC1, which is known to contrast pain and neuroinflammation, can prevent mood alterations. Mice were treated with BTZ (0.4 mg/kg three times/week for 4 weeks); mechanical allodynia and locomotor activity were evaluated over time while anxiety (dark light and marble burying test), depression (sucrose preference and swimming test) and supraspinal neuroinflammation were checked at the end of the protocol. BTZ treated neuropathic mice develop anxiety and depression. The presence of mood alterations is related to the presence of neuroinflammation and PK system activation in prefrontal cortex, hippocampus and hypothalamus with high levels of PK2 and PKR2 receptor, IL‐6 and TNF‐α, TLR4 and an upregulation of glial markers. PC1 treatment, counteracting pain, prevented the development of supraspinal inflammation and depression‐like behavior in BTZ mice