POSTPARTUM ELEVATED Β-HYDROXYBUTYRATE AND NON-ESTERIFIED FATTY ACIDS TOGETHER OR SEPARATELY AND THEIR ASSOCIATION WITH PLASMA METABOLITES, BODY CONDITION AND REPRODUCTIVE PERFORMANCE IN DAIRY COWS

This study aimed to assess post-partum elevated nonesterified fatty acids (NEFA) and β hydroxybutyrate (BHB), considered either togetheror separately,relative to the estrus cyclicity and first service pregnancy status of cows and their association withbody condition scores and some metabolites.Blood samples from 50 Montbéliarde dairy cowswere collected from 15 to 52 DIMto measure serum  BHB, NEFA,glucose, triglycerides, total cholesterol, urea nitrogen, total protein, aspartate aminotransferase (AST), alanine aminotransferase (ALT), γ-glutamyltransferase (γGT), calcium, magnesium, potassium, phosphorus, sodium;and progesterone concentrations.Body condition score (BCS) was assessed at calving and at each time when blood samples were taken.Cows were considered as having post-partum elevated NEFA (H-NEFA) concentration if the concentration was≥0.70 mM and post-partum elevated BHB (H-BHB) concentration if the concentration was≥1.20 mM at 30 DIM. Overall, 93.33 % of cows having an elevated BHB show an elevated of NEFA and 51.61% of cows having an elevated NEFA have not an elevated BHB. Indeed, considering postpartum elevated NEFA as a predictor of sub-clinical ketotic cows can overrateresults. Whereas, considering postpartum elevated BHB as a predictor of cows with NEB can underestimate results. Excessive BCS at calving results in increasing the risk of post-partum elevated BHB. Cholesterol, triglycerides, AST, ALT, and urea were increased in cows having elevated BHB and NEFA compared with those having elevated NEFA only or healthy cows. Further, the risk of estrus cyclicity and pregnancy rate at first insemination (P/AI) was decreased in cows having both elevated BHB and NEFA or NEFA only

Induction of Human Immunodeficiency Virus (HIV-1) Envelope Specific Cell-Mediated Immunity by a Non-Homologous Synthetic Peptide

Peer reviewed: YesNRC publication: Ye

Mortality from gastrointestinal congenital anomalies at 264 hospitals in 74 low-income, middle-income, and high-income countries: a multicentre, international, prospective cohort study

Summary Background Congenital anomalies are the fifth leading cause of mortality in children younger than 5 years globally. Many gastrointestinal congenital anomalies are fatal without timely access to neonatal surgical care, but few studies have been done on these conditions in low-income and middle-income countries (LMICs). We compared outcomes of the seven most common gastrointestinal congenital anomalies in low-income, middle-income, and high-income countries globally, and identified factors associated with mortality. Methods We did a multicentre, international prospective cohort study of patients younger than 16 years, presenting to hospital for the first time with oesophageal atresia, congenital diaphragmatic hernia, intestinal atresia, gastroschisis, exomphalos, anorectal malformation, and Hirschsprung’s disease. Recruitment was of consecutive patients for a minimum of 1 month between October, 2018, and April, 2019. We collected data on patient demographics, clinical status, interventions, and outcomes using the REDCap platform. Patients were followed up for 30 days after primary intervention, or 30 days after admission if they did not receive an intervention. The primary outcome was all-cause, in-hospital mortality for all conditions combined and each condition individually, stratified by country income status. We did a complete case analysis. Findings We included 3849 patients with 3975 study conditions (560 with oesophageal atresia, 448 with congenital diaphragmatic hernia, 681 with intestinal atresia, 453 with gastroschisis, 325 with exomphalos, 991 with anorectal malformation, and 517 with Hirschsprung’s disease) from 264 hospitals (89 in high-income countries, 166 in middleincome countries, and nine in low-income countries) in 74 countries. Of the 3849 patients, 2231 (58·0%) were male. Median gestational age at birth was 38 weeks (IQR 36–39) and median bodyweight at presentation was 2·8 kg (2·3–3·3). Mortality among all patients was 37 (39·8%) of 93 in low-income countries, 583 (20·4%) of 2860 in middle-income countries, and 50 (5·6%) of 896 in high-income countries (p<0·0001 between all country income groups). Gastroschisis had the greatest difference in mortality between country income strata (nine [90·0%] of ten in lowincome countries, 97 [31·9%] of 304 in middle-income countries, and two [1·4%] of 139 in high-income countries; p≤0·0001 between all country income groups). Factors significantly associated with higher mortality for all patients combined included country income status (low-income vs high-income countries, risk ratio 2·78 [95% CI 1·88–4·11], p<0·0001; middle-income vs high-income countries, 2·11 [1·59–2·79], p<0·0001), sepsis at presentation (1·20 [1·04–1·40], p=0·016), higher American Society of Anesthesiologists (ASA) score at primary intervention (ASA 4–5 vs ASA 1–2, 1·82 [1·40–2·35], p<0·0001; ASA 3 vs ASA 1–2, 1·58, [1·30–1·92], p<0·0001]), surgical safety checklist not used (1·39 [1·02–1·90], p=0·035), and ventilation or parenteral nutrition unavailable when needed (ventilation 1·96, [1·41–2·71], p=0·0001; parenteral nutrition 1·35, [1·05–1·74], p=0·018). Administration of parenteral nutrition (0·61, [0·47–0·79], p=0·0002) and use of a peripherally inserted central catheter (0·65 [0·50–0·86], p=0·0024) or percutaneous central line (0·69 [0·48–1·00], p=0·049) were associated with lower mortality. Interpretation Unacceptable differences in mortality exist for gastrointestinal congenital anomalies between lowincome, middle-income, and high-income countries. Improving access to quality neonatal surgical care in LMICs will be vital to achieve Sustainable Development Goal 3.2 of ending preventable deaths in neonates and children younger than 5 years by 2030

Identification of oligopeptides from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) non structural protein 8 (NSP8) and their similarities with type 1 angiotensin II receptor key sites

Coronavirus disease 2019 is associated with clinical symptoms including severe inflammatory syndrome and a higher expression of angiotensin II. As a pro-inflammatory mediator, the physiologic effects of angiotensin II are mediated by a G-protein coupled receptor, termed AT1R. Following binding, AT1R initiates the process of signal desensitization necessary to maintain cellular homeostasis. At the cellular level, this function occurs via the G protein-dependent signaling and the phosphorylation. We describe amino acids similarities between SARS COV-2 nonstructural protein (NSP8) which is associated with intracellular membranes and AT1R key sites. Since abnormal activation of AT1R receptor leads to a number of physiological disorders, we hypothesize that SARS COV-2 might further interfere with the angiotensin II receptor functions

MPI-PERF-SIM: Towards an automatic performance prediction tool of MPI programs on hierarchical clusters

International audienceWe present in this paper a framework for performance prediction of parallel programs on hierarchical clusters. This framework is mainly designed for the use by the switching functions in parallel adaptive applications. Indeed, the principal referred objectives by this framework are the accuracy of the prediction and the rapidity of the prediction process. To achieve these objectives, our framework is based on two principal steps, the first is at the installation moment of the parallel application, and the second is at runtime. In the first step, we profile two components which are sequential kernels of the program and network performances. In order to model accurately these two components we have developed new strategies of regression. In the second step, we use the generated models and the runtime variables to the completion time estimation via our fast simulator MPI-PERF-SIM. Our experimentations on the Grid'5000 platform demonstrate the interest of this approach that can be the basis of adaptivity for parallel numerical libraries on dedicated hierarchical platforms

Study of the mechanical behavior and durability of mortars based on prepared sand

Sand occupies a great proportion of the cementitious matrix product and in particular mortars. Hence, the study of fine aggregates used for concrete and mortar in general, deserves to be objects of research including sand which has always been considered as inert material, whose role is exclusively physical. The study of mortars performances based on prepared sand to assess the effect of the type and rate of substitution of mineral additions pozzolana and blast furnace slag of a natural sand fine fraction (sieve diameter less than 0.16 mm) is seen as the main objective of the present experimental research work. The natural sand replaced size is less than 160 µm and for rheological reasons the maximum replacement rate of natural sand is limited to 10%. The results obtained show a significant improvement of the mechanical properties for the mortars based on the new activated sand. With regard to durability tests of HCl and H2SO4 acids chemical attacks, the substitution of the quartz by active mineral additions in the sand-size skeleton allows an advantageous reduction in loss of resistance up to 50% and a mass gain around 75%

Pleiotrophin induces expression of inflammatory cytokines in peripheral blood mononuclear cells.

Pleiotrophin (PTN) is a polypeptide that belongs to a family of heparin-binding growth factor, which displays mitogenic activity for a wide variety of cells. Since PTN induces the proliferation of immune cells the mechanism of action was investigated. In the present study, we show for the first time that PTN induces the expression of inflammatory cytokines including TNF-alpha, IL-1beta and IL-6 in quiescent human peripheral blood mononuclear cells (PBMC). These results emphasize the importance of PTN in the regulation of inflammatory processes. Elucidation of the mechanisms by which a host factor such PTN regulates cytokines production will significantly advance our understanding of endothelium-immunity interactions

T cell survival/proliferation reconstitution by trifluoperazine in human immunodeficiency virus-1 infection

AbstractRecent findings support an indirect relationship between T cell depletion in HIV-1 infection and the rate of virus replication with implications for treatment strategies. We have initiated a new approach to recover immune function through the use of novel chemical agents. A cationic amphiphilic drug that binds to Ca2+-calmodulin at high concentrations, [10-{3-(4-methyl-1-piperazinyl)-propyl}-2- (trifluoromethyl)-10H-phenothiazine dihydrochloride] [denoted trifluroperazine dihydrochloride (Tfp); molecular weight 480.43] TFP was found at low concentrations (10−6 to 10−10 M) to help T cells from AIDS patients to restore proliferation in vitro. Here we show that the Tfp molecule can restore the cell survival of T lymphocytes from PBMCs derived from HIV-1-infected patients in vitro. Tfp enhances T cell proliferation and Th-cell responses by selectively inhibiting cell mortality and apoptosis. The restored antigen-specific response is associated with the synthesis of IL-2 and γ-interferon. Even though this drug does not possess any detectable antiviral effect, it might be considered as a potential therapeutic agent in HIV-infected patients, to correct immune defects. Besides antiviral compounds, these data may facilitate immune reconstitution in patients with HIV infection and other immunosuppressive diseases