Rapid in vitro Propagation of Boerhaavia diffusa (L.) through Nodal Segments

 A rapid and efficient protocol for the large scale propagation of a potential medicinal plant Boerhaavia diffusa L., through in vitro culture of nodal segment explants obtained from aseptic seedlings. In vitro multiple shoot (14-15) induction was observed from axillary bud explants cultured on MS medium fortified with BAP (2.0 mg/l) and Kn (3.5 mg/l). The multiple shoots were separated and subcultured for their elongation on same medium supplemented with gibberelic acid (0.5 mg/l). Rooting on in vitro produced elongated shoots was achieved on half MS medium having IBA (0.5 mg/l). Rooted plantlets were hardened in plastic pots containing sterilized soil and vermiculite (3:1). Well established plantlets were acclimatized to the field with 70% survival rate.  Key words: Medicinal plants, Nodal segments, Micropropagation, in vitro rooting  Plant biotechnology laboratory, Department of Botany, University of Rajasthan, Jaipur 302004, Rajastha

Household remedies of Keshavraipatan tehsil in Bundi district, Rajasthan

362-367The paper presents ethnomedicinal remedies for joints pain, arthritis, diabetes, asthma, swollen gums, dermatitis, ear, eye and hair problems, pneumonia, hypo-pigmentation, snakebite, scorpion bite, piles, mouth sore, dysentery, gastric and urinary problems, jaundice, whooping cough, bone fracture, swelling, cardiac and liver problems, etc. practiced by rural population of Keshavraipatan tehsil of Bundi district. In all, 54 plant species belonging to 35 families used in the treatment have been enumerated giving botanical and local names, mode of preparation, dosage and usages

An alternative source for regenerable organogenic callus induction in <i style="">Jatropha curcas</i> L.

545-548Plant regeneration of Jatropha curcas was achieved through organogenesis in callus cultures. Calli were induced from leaf explants on MS basal medium supplemented with NAA (1.0 mg/L) and BAP (5.0 mg/L). Green compact nodules containing clusters of meristematic centres were induced in these calli after transfer to MS basal medium containing various concentrations of BAP/Kn (0.5-5.0 mg/L) alone or in combination with auxins. MS medium supplemented with BAP (1.5 mg/L) and IBA (0.5 mg/L) was found to be the most effective combination for shoot bud differentiation. The microshoots rooted well on MS+IBA (3.0 mg/L) medium and the plantlets successfully acclimatized in soil

High frequency <i>in vitro</i> shoot regeneration of <i>Clitoria ternatea</i> L. affected by different cultural conditions

210-214An efficient in vitro propagation system was established for direct shoot regeneration in Clitoria ternatea L.. The regeneration protocol was standardized by using different explants, viz., nodal, cotyledonary node and shoot tip, on Murashige and Skoog (MS) medium with different concentrations of sucrose (1-6% ), plant growth regulators BA and Kn (1.0-6.0 µM), and different levels of pH (5.2-6.2). The highest mean number of shoots (18.7±1.0) was obtained from nodal explants on MS medium containing 3% sucrose and 0.8% agar supplemented with BA (5.0 µM) at pH 5.8. In vitro regenerated and elongated shoots were rooted on ½ MS medium fortified with IBA (2.0 µM). Complete plantlets were then hardened, acclimatized and transplanted to natural conditions, where they exhibited 80% survivability

Micropropagation of <i>Salvadora persica </i>Linn. via Cotyledonary Nodes

197-200An efficient and reliable protocol for micropropagation of Salvadora persica Linn. has been standardized, which is a medicinally as well as economically important arid zone plant species. Cotyledonary nodes (1cm long) excised from 15-20 days-old seedlings germinated in vitro served as explant source. The seeds were germinated on half strength MS medium devoid of phytohormones. Cotyledonary nodes were cultured on MS medium supplemented with different concentrations of cytokinins (BAP and KN) and auxins (IAA, IBA and NAA). Maximum shoot proliferation from single explant was obtained on MS medium incorporated with BAP (4.0 mg/l), IAA (0.5 mg/l), adenine sulphate (40 mg/l), glutamine (l00 mg/l) and thiamine HCl (10 mg/l). In vitro produced shoots were induced to root on a range of IBA concentrations (0.5-5.0 mg/l) supplemented to half strength MS medium. The highest frequency of root proliferation was on half strength MS medium supplemented with 3.0 mg/l IBA. The regenerates were transferred to field conditions after acclimatization with a success rate of 60%

BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY A N I N T E R N A T I O N A L J O U R N A L Comparative Microbicidal Efficacy of Fractionated Extracts from In Vitro and In Vivo Raised Cells of Tinosporacordifolia Against MDR Pathogens

ABSTRAC

Plant regeneration and stimulation of in vitro flowering in Eruca sativa Mill.

Explants such as apical buds, axillary buds, cotyledons, cotyledonary nodes, leaves, hypocotyls and immature embryonal axes from in vitro-grown plantlets were inoculated on the Murashige and Skoog (MS) medium supplemented with 4.44 μM 6-benzylaminopurine in combination with 2.85 μM indole-3-acetic acid. Best multiple shoots formation was obtained with cotyledonary nodes. Each inoculated explant produced 18.10 ± 0.66 shoots within 2 to 3 weeks. These shoots were separated carefully and were transferred to the fresh half strength MS solid medium with indole-3-butyric acid (4.90 μM) for the development of the roots. These in vitro-developed plantlets produced flowers on the same medium with supplementation of 6-furfuril kinetin (0.23 μM). These plantlets were successfully transferred to the soil where they grew well for 8 to 10 weeks with 80% survivability.Keywords: Eruca sativa, cotyledonary nodes, in vitro regeneration, in vitro flowering, shoot multiplicatio