The Expression of Heat Shock Protein 70 Gene with Organic Selenium Supplementation and Its Effetc on Productivity of Broilers in Tropical Environment

The purpose of this experiment is to study the effect of organic selenium (Se) supplementation on the expression of heat shock protein 70 gene (HSP70), glutathione peroxidase (GSH-Px) enzyme activity, malondialdehyde (MDA) and productivity of broilers in tropical environment. Three kinds of environmental pens were designed in this experiment: comfortable environment pens with temperature of air conditioner adjusted at 22oC (R0), tropical environment pens (±30oC ) without organic Se (R1), and tropical environment pens supplemented with 0.30 ppm organic Se (R2). One hundred and twenty broiler chickens (unisex) were used in this study. There were 40 chicks per pen for each treatment. The experimental design was completely randomized with four replications for each treatment. The data were statistically analyzed using the general linear model of SAS program. Results showed that R0 and R2 groups had significantly increased (P<0.05) feed intake, body weight, body weight gain, and decreased feed conversion ratio compared to R1 groups. Meanwhile, the expression of HSP70, GSH-Px enzyme activity and MDA of R2 groups and R0 groups were significantly lower (P<0.05) than that of R1 groups. It was concluded that the broilers given 0.30 ppm organic Se in tropical environment had similar productivity and expression of HSP 70 with broilers kept in comfortable environment

Effect of Bacillus amyloliquefaciens as probiotic on total colonies of bacteria, pH and cellulase activity in the small intestine of domestic chicken

This study aimed to determine the effect of the addition of Bacillus amyloliquefaciens into drinking water on the total colonies of Bacillus amyloliquefaciens, Lactobacillus sp, pH, and cellulase activity in the small intestine of domestic chicken in starter period. This study was used 200 days old of domestic chicken CP 808 kept for 8 weeks. Type of cage used 20 units cage measuring 85 cm x 70 cm x 30 cm which each unit contained 10 chickens. The study used the Completely Randomized Design (CRD) with 4 treatments in 5 replications. Treatment was the addition of B. amyloliquefaciens into drinking water (without B. amyloliquefaciens, 45 x 109 CFU/ml, 65x1010 CFU/ml, and 43 x 1012 CFU/ml). The variables observed were the total colonies of B. amyloliquefaciens, Lactobacillus sp, pH, and cellulase activity in the small intestine of domestic chicken. The results showed that addition of B. amyloliquefaciens in drinking water of domestic chicken increased the total colonies of B. amyloliquefaciens, Lactobacillus sp, and cellulase activity in the small intestine, but did not affect the pH. The highest total colonies (B. amyloliquefaciens and Lactobacillus sp) and cellulase activity were found on the addition of B. amyloliquefaciens at a level of 43 x 1012 CFU/gram. Meanwhile, the pH intestine added B. amyloliquefaciens tended to be lower than without B. amyloliquefacien

Performa Puyuh (Coturnix coturnix japonica) yang Disuplementasi Susu Bubuk Kadaluarsa pada Tingkat Protein Berbeda

Penelitian bertujuan untuk mengetahui performa puyuh (Coturnix coturnix japonica) yang suplementasi susu bubuk kadaluarsa pada tingkat protein berbeda terhadap performa puyuh. Puyuh yang digunakan yaitu puyuh betina petelur (Coturnix coturnix japonica) periode pertumbuhan umur 21 hari sebanyak 240 ekor. Penelitian menggunakan metode Rancangan Acak Lengkap (RAL) pola faktorial 2x3 dengan 4 ulangan, setiap ulangan terdiri dari 10 ekor puyuh petelur. Penelitian terdiri dari dua faktor yaitu level kebutuhan protein (20% dan 18%) sebagai faktor pertama dan suplementasi susu bubuk kadaluarsa (0%; 0,25%; 0,50%) sebagai faktor kedua. Peubah yang diamati yaitu konsumsi ransum (g/ekor/hari), pertambahan bobot badan (g/ekor/hari), konversi ransum, dan umur pertama bertelur. Data yang diperoleh dianalisis dengan ANOVA, jika berbeda nyata diuji lanjut dengan DMRT. Hasil anasisis ragam menunjukkan bahwa suplementasi susu bubuk kadaluarsa dalam ransum berpengaruh tidak nyata (P>0,05) terhadap konsumsi ransum, pertambahan bobot badan, konversi ransum, dan umur pertama bertelur. Dapat disimpulkan bahwa pada level protein 18% dengan penambahan susu bubuk kadaluarsa sebanyak 0,50% dalam ransum puyuh periode pertumbuhan mampu mempertahankan performa puyuh dengan nilai konsumsi ransum 12,64 g/ekor/hari, pertambahan bobot badan 2,65 g/ekor/hari, konversi ransum 5,23, dan umur pertama bertelur 48 hari