Biological Particle Control and Separation using Active Forces in Microfluidic Environments

Exploration of active manipulation of bioparticles has been impacted by the development of micro-/nanofluidic technologies, enabling evident observation of particle responses by means of applied tunable external force field, namely, dielectrophoresis (DEP), magnetophoresis (MAG), acoustophoresis (ACT), thermophoresis (THM), and optical tweezing or trapping (OPT). In this chapter, each mechanism is presented in brief yet concise, for broad range of readers, as strong foundation for amateur as well as brainstorming source for experts. The discussion covers the fundamental mechanism that underlying the phenomenon, presenting the theoretical and schematic description; how the response being tuned; and utmost practical, the understanding by specific implementation into bioparticles manipulation engaging from micron-sized material down to molecular level particles

Приклади завдань для позакласної роботи з інформатики у початковій школі

У статті коротко описана методика проведення позакласної роботи з інформатики у початковій школі

Dielectrophoretic deformation of breast cancer cells for lab on a chip applications

This paper presents the development and experimental analysis of a curved microelectrode platform for the DEP deformation of breast cancer cells (MDA-MB-231). The platform is composed of arrays of curved DEP microelectrodes which are patterned onto a glass slide and samples containing MDA-MB-231 cells are pipetted onto the platform's surface. Finite element method is utilised to characterise the electric field gradient and DEP field. The performance of the system is assessed with MDA-MB-231 cells in a low conductivity 1% DMEM suspending medium. We applied sinusoidal wave AC potential at peak to peak voltages of 2, 5, and 10 Vpp at both 10 kHz and 50 MHz. We observed cell blebbing and cell shrinkage and analyzed the percentage of shrinkage of the cells. The experiments demonstrated higher percentage of cell shrinkage when cells are exposed to higher frequency and peak to peak voltage electric field.</p

Dielectrophoretic deformation of breast cancer cells for lab on a chip applications

This paper presents the development and experimental analysis of a curved microelectrode platform for the DEP deformation of breast cancer cells (MDA-MB-231). The platform is composed of arrays of curved DEP microelectrodes which are patterned onto a glass slide and samples containing MDA-MB-231 cells are pipetted onto the platform's surface. Finite element method is utilised to characterise the electric field gradient and DEP field. The performance of the system is assessed with MDA-MB-231 cells in a low conductivity 1% DMEM suspending medium. We applied sinusoidal wave AC potential at peak to peak voltages of 2, 5, and 10 Vpp at both 10 kHz and 50 MHz. We observed cell blebbing and cell shrinkage and analyzed the percentage of shrinkage of the cells. The experiments demonstrated higher percentage of cell shrinkage when cells are exposed to higher frequency and peak to peak voltage electric field

Customized Two-Dimensional Nanostructured MoO₃ Inks For Spectrally Selective UV Chromic Patches

Ultraviolet (UV) radiation exposure plays an important role in human health. However, excessive exposure can lead to skin injuries and melanoma. It is crucial to have a technology that can monitor exposure, particularly to UV A, which penetrates the human skin the most and also assists in building healthy sun habits in the general population. Current sensors do not offer the needed combination of narrow optical absorbance, ease of use, and low cost. Here, we report MoO3 photochromic inks that are customized to the skin complexion based on Fitzpatrick’s skin typing system. The ink is subsequently incorporated into bioelastomers to make “stick-on” UV patches that provide a qualitative assessment of UV A exposure to a user on the go. The photochromism of the patch upon UV exposure is correlated to the calculated percentages of the minimal erythema dose (MED) limits. The patch gives a visual indication of the levels of UV A exposure, which will allow the users to make informed choices to avoid overexposure and prevent skin damage. The patch is fabricated using a manufacturing-compatible screen printing process. This technology has the potential to drive behavioral changes in the population and move toward an aware and sun-smart community

Dielectrophoresis-based microfluidic platforms for cancer diagnostics

The recent advancement of dielectrophoresis (DEP)-enabled microfluidic platforms is opening new opportunities for potential use in cancer disease diagnostics. DEP is advantageous because of its specificity, low cost, small sample volume requirement, and tuneable property for microfluidic platforms. These intrinsic advantages have made it especially suitable for developing microfluidic cancer diagnostic platforms. This review focuses on a comprehensive analysis of the recent developments of DEP enabled microfluidic platforms sorted according to the target cancer cell. Each study is critically analyzed, and the features of each platform, the performance, added functionality for clinical use, and the types of samples, used are discussed. We address the novelty of the techniques, strategies, and design configuration used in improving on existing technologies or previous studies. A summary of comparing the developmental extent of each study is made, and we conclude with a treatment of future trends and a brief summary