Validity of naked eye single tube red cell osmotic fragility test (NESTROFT) in screening of beta-thalassemia trait

زمینه و هدف: تالاسمی متداولترین اختلال تک ژنی است که رهایی از آن از طریق درمان قطعی ممکن نبوده و مستلزم پیشگیری از طریق به کارگیری یک روش قابل اعتماد و کم هزینه برای غربالگری ناقلین و در مرحله بعد ارایه آموزش، مشاوره ژنتیک، تشخیص قبل از تولد و خاتمه انتخابی به زندگی جنین های مبتلا به این اختلال است. هدف از این مطالعه ارزیابی تست اسموتیک تک لوله ای چشمی گلبول های قرمز (NESTROFT) به عنوان یک تست غربالگری در راستای کشف مبتلایان به بتاتالاسمی مینور بود. روش بررسی: در این مطالعه توصیفی- تحلیلی، تست NESTROFT بر روی 158 نفر متشکل از 51 نفر فرزندان والدینی که حداقل یکی از بچه های آنها دارای بتا تالاسمی ماژور بود، 51 فرد طبیعی و 56 فرد از مبتلایان به فقر آهن انجام شد. داده های حاصل به کمک آزمون های آماری آنالیز واریانس یک راهه و آزمون تعقیبی دانت تجزیه و تحلیل گردید. یافته ها: بر اساس نتایج، حساسیت و ارزش اخباری منفی تست NESTROFT 100 بوده و با توجه به 14 مورد نتیجه مثبت کاذب ناشی از فقر آهن، ویژگی آن 9/86 و ارزش اخباری مثبت تست 5/78 می باشد. نتیجه گیری: تست NESTROFTدر عین کم هزینه بودن و سهولت انجام، حساسیت بالایی برای کشف بتا تالاسمی مینور داشته و می توان از آن در کشورهای در حال توسعه با منابع اقتصادی و تکنیکی محدود از قبیل ایران در مقیاس وسیع جهت غربالگری توده ای استفاده نمود

Study of -629C/A polymorphism of cholesteryl ester transfer protein gene in statin effects on plasma high density lipoprotein cholesterol level

Background and aim: Cholesteryl ester transfer protein (CETP) plays pivotal role in HDL metabolism and in reverse cholesterol transport (RCT) pathway. CETP gene variants such as -629C/A that affect HDL cholesterol directly modulates CETP gene tranh1ional activity. This study was aimed to determine influence of -629C/A polymorphism of CETP in statin effects with regard to plasma HDL cholesterol levels. Methods: In this deh1ive-analytical study 196 adult patients with LDL-C more than 120mg/dL were divided into two groups base on lovastatin and atorvastatin using. Lipid profile was measured in all subjects before and after treatment and -629C/A polymorphism of CETP promoter was studied using polymerase chain reaction/restriction fragment length polymorphism method. Data were compared with paired t-test and ANOVA in SPSS software. Results: Cholesterol was decreased and HDL was increased in AA genotype more than other genotypes by lovastatin but ApoA1 was increased in CC genotype. ApoA1 also was increased in CC genotype more than AA or AC genotypes by atorvastatin. Conclusion: In CC genotype lovastatin and specially atorvastatin increased ApoA1 in HDL particles more than other genotypes. Therefore treatment with lovastatin and atorvastatin is more effective in patients with CC genotype for raising HDL particles activity

Study of -629C/A polymorphism of cholesteryl ester transfer protein gene in statin effects on plasma high density lipoprotein cholesterol level

Background and aim: Cholesteryl ester transfer protein (CETP) plays in HDL metabolism and in reverse cholesterol transport (RCT) pivotal role pathway. CETP gene variants such as -629C/A that affect HDL cholesterol directly, modulates CETP gene transcriptional activity. This study was aimed to determine influence of -629C/A polymorphism of CETP in statin effects with regard to plasma HDL cholesterol levels. Methods: In this descriptive-analytical study, 196 adult patients with LDL-C more than 120mg/dL were divided into two groups base on lovastatin and atorvastatin using. Lipid profile was measured in all subjects before and after treatment and -629C/A polymorphism of CETP promoter was studied using polymerase chain reaction/restriction fragment length polymorphism method. Data were compared with paired t-test and ANOVA in SPSS software. Results: Cholesterol was decreased and HDL was increased in AA genotype more than other genotypes by lovastatin, but ApoA1 was increased in CC genotype. ApoA1 also was increased in CC genotype more than AA or AC genotypes by atorvastatin. Conclusion: In CC genotype, lovastatin and specially atorvastatin increased ApoA1 in HDL particles more than other genotypes. Therefore, treatment with lovastatin and atorvastatin is more effective in patients with CC genotype for raising HDL particles activity

Hydroxypropyl beta cyclodextrin: a water-replacement agent or a surfactant upon spray freeze-drying of IgG with enhanced stability and aerosolization

The great potential of hydroxypropyl beta-cyclodextrin (HP�CD), as a dried-protein stabilizer, has been attributed to various mechanisms namely water-replacement, vitrification and surfactant-like effects. Highlighting the best result in our previous study (weight ratio IgG: HP�CD of 1:0.4), herein we designed to evaluate the efficacy of upper (1:2) and lower (1:0.05) ratios of HP�CD in stabilization and aerosol properties of spray freeze-dried IgG. The protective effect of HPβCD, as measured by size exclusion chromatography (SEC-HPLC) was most pronounced at C3� and C3�, IgG:trehalose:HPβCD ratios of 1:2:0.25 and 1:2:0.05 with aggregation rate constants of 0.46 ± 0.02 and 0.58 ± 0.01 (1/month), respectively. The secondary conformations were analyzed through Fourier transform infrared spectroscopy (FTIR) and all powders well-preserved with the lack of any visible fragments qualified through sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PPAGE). Scanning electron microscopy (SEM) and twin stage impinger (TSI) were employed to characterize the suitability of particles for further inhalation therapy of antibodies and the highest values of fine particle fraction (FPF) were achieved by C3� and C3�, 56.43 and 48.12. The powders produced at the current ratio 1:2:0.25 and 1:2:0.05 are superior to our previous examination with regards to manifesting lower aggregation and comparable FPF values. © 2020, © 2020 Informa UK Limited, trading as Taylor & Francis Group