Modernization using the structured system design of the Bhadra Reservoir Project, India: an intervention analysis

Performance evaluationIrrigation programsModernizationParticipatory managementFarmer participationFarmer-agency interactionsFarmers' attitudesRemote sensingRiceIrrigated farmingWater distributionWater supplyProductivity

Effect of residual detergents in specimen collection containers on routine serum biochemical analytes

The use of Teepol, Lysol and sodium hypochlorite are frequent in reusing of specimen collection tubes by the state hospitals in Sri Lanka. Detergent residue in collection tubes is   thought to be the reason for uncertain results in the recent past. This raised a concern to evaluate the effect of washing of the specimen collection tubes by various detergents on serum creatinine, aspartate transaminase (AST), Na+ and K+. Three sets of newly purchased glass Khan tubes were washed using Teepol, Lysol (concentration of 1%), and sodium hypochlorite (0.1 %) adhering to World Health Organization protocol. Blood from a single donor was aliquoted to a detergent washed tubes (test) and to a newly purchased plain glass Khan tube (control). Both were tested for serum creatinine, AST, Na+ and K+. A sample size of 20 was analyzed for each detergent-washed tube. There was no significant difference in serum creatinine, AST and K+ when Teepol, Lysol or sodium hypochlorite washed tubes were used in sequence (p>0.05). Na+ concentrations measured in serum was significantly different in specimens collected to Lysol-washed tubes (p<0.05). The Lysol-washed tubes were significantly contaminated with detergent residues. In conclusion, domestic detergents (Teepol, Lysol and sodium hypochlorite) don’t impart a significant effect on tested analytes if the cleansing is done according to guidelines by World Health Organization. However, newly purchased tubes are recommended in critical investigations such as serum electrolytes to improve the accuracy of laboratory reports.         KEYWORDS:  Teepol, Lysol, Sodium hypochlorite, creatinine, Na+ and K+ &nbsp

Observations on metal concentrations in commercial landings of two species of tilapia (Oreochromis mossambicus and Oreochromis niloticus) from reservoirs in six river basins in Sri Lanka

Samples of the muscle of two species of tilapia (Oreochromis mossambicus and O. niloticus; 17-20 cm length) were obtained from at least one reservoir in each of the six river basins (Aruvi Aru, Kala Oya, Kirindi Oya, Ma Oya, Mahaweli, and Walawe Ganga catchments) in Sri Lanka. The metals Ca, Cu, Fe, K, Mg, Mn, Na, and Zn were consistently detected in the muscle tissue. Overall, there were few differences in the concentration of metals between the two species of fish, although there were also some statistically significant differences (p &lt; 0.05) in the concentrations of some metals in fish obtained from some of the reservoirs. Aruvi Aru stands out as a river basin in which the two fish species have significantly lower concentration of metals when compared to other river basins. The concentration of the metals studied were below WHO and FSANZ guideline values for fish, suggesting that the consumption of the metals found in tilapia from these reservoirs poses little risk to human health. <br /

The evolutionary origin of CIPK16: a gene involved in enhanced salt tolerance

Abstract not availableShanika Amarasinghe, Nathan S. Watson-Haigh, Matthew Gilliham, Stuart Roy, Ute Bauman

Investigation of Socioeconomic Characteristics of Agricultural Communities in Relation to the Development of Culture-Based Fisheries in Non-Perennial Reservoirs of Sri Lanka

Participation of community groups is one of the main requirements for culture-based fisheries (CBF) in non-perennial reservoirs of Sri Lanka. Homogeneity of the group characteristics facilitates to arrive at collective decisions. And as such can be considered as positive feature for development of CBF. In this paper an attention made to identify the most important social and economic characteristics affecting the group decisions for development and management of CBF in non-perennial reservoirs of Sri Lanka. Principal component analysis (PCA) revealed that out of 25 measured parameters only 4 namely, the group size of fishers, participation rate in fisheries activities, caste of community and participation rate for regular meetings, were important in determining socioeconomic heterogeneity of the sample. Average income derived from CBF was appreciably high in communities with small group size and high participatory level in-group activities. Contingent evaluation of willingness to pay also demonstrated that the involvement of more people with fishing attitude would be preferred for the successful CBF activities. This analysis indicates that socioeconomic characteristics of rural communities can be meaningfully used for selecting non-perennial reservoirs suitable for development of CBF in Sri Lanka

Preliminary study on herpetofaunal diversity of Nilgala forest area in Monaragala district, Sri Lanka

Nilgala ForestArea (NFA) is one of the largest and important forest area in Monaragala District, UvaProvince. It contain 12,432 hectares and lies within 7° 08' - 7° 14' NL and 81 D 16' - 81020' EL. Itselevation range between 200m to 700m within the Irindahela, Hangale, Yakun hela (highest point 700rn), Hamapola, Badangarnuwa, Keenagoda, Makada, Karadugala, Kukulagoda, Ewalahela, Gorikkadahills. The mean annual rainfall varies between where the average annual rainfall 1500mm - 2000mm(rain during northeast monsoon). while the mean annual temperature of the area is 28 DC - 31°C.The vegetation comprised with lowland tropical moist semi evergreen forest and savannah forest,home gardens and small patch paddy cultivations. The dominant tree species are Aralu (Terminaliachebulav, Bulu iTerminalia bellirica) and Nelli iPhyllanthus emblicay. Other than biodiversity,Nilgala is rich of archaeological monuments, such as prehistoric, proto-historic and historical Buddhistmonasteries.During the two-year study period, total number of 70 reptile species were and 19 amphibian speciesrecorded. Reptiles include 44 genera of 17 families and 20 (28.5%) endemic species. Amphibianfauna contain 13 genera including 4 families and 6 (31.5%) endemic species. 41.4% (29) of reptilesand 26.3% (5) of Amphibians listed as 'Nationally Threatened' in the 1999 IUCN National threatenedlist. Out of70 species 38 (54.2%) are Serpentoid reptiles (11 endemics) and 32 (45.7%) species areof Tetrapod reptiles (9 endemics). Among the recorded species, 11 Serpentoid, 3 Tetrapod, and 2amphibians have not been recorded by previous workers. Furthermore seven unidentified specieswere also recorded during the survey, which probably include new amphibian species belonging togenus Nannophrys. Human activities such as man-made fire, ilIegallogging, extensive use of chemicalsfor agriculture, forest clearing for chena cultivation and road kills were identified as a main threat forthe natural habitats as well as faunal species.

Global Regulatory Functions of the Staphylococcus aureus Endoribonuclease III in Gene Expression

RNA turnover plays an important role in both virulence and adaptation to stress in the Gram-positive human pathogen Staphylococcus aureus. However, the molecular players and mechanisms involved in these processes are poorly understood. Here, we explored the functions of S. aureus endoribonuclease III (RNase III), a member of the ubiquitous family of double-strand-specific endoribonucleases. To define genomic transcripts that are bound and processed by RNase III, we performed deep sequencing on cDNA libraries generated from RNAs that were co-immunoprecipitated with wild-type RNase III or two different cleavage-defective mutant variants in vivo. Several newly identified RNase III targets were validated by independent experimental methods. We identified various classes of structured RNAs as RNase III substrates and demonstrated that this enzyme is involved in the maturation of rRNAs and tRNAs, regulates the turnover of mRNAs and non-coding RNAs, and autoregulates its synthesis by cleaving within the coding region of its own mRNA. Moreover, we identified a positive effect of RNase III on protein synthesis based on novel mechanisms. RNase III–mediated cleavage in the 5′ untranslated region (5′UTR) enhanced the stability and translation of cspA mRNA, which encodes the major cold-shock protein. Furthermore, RNase III cleaved overlapping 5′UTRs of divergently transcribed genes to generate leaderless mRNAs, which constitutes a novel way to co-regulate neighboring genes. In agreement with recent findings, low abundance antisense RNAs covering 44% of the annotated genes were captured by co-immunoprecipitation with RNase III mutant proteins. Thus, in addition to gene regulation, RNase III is associated with RNA quality control of pervasive transcription. Overall, this study illustrates the complexity of post-transcriptional regulation mediated by RNase III