Infecções associadas a felinos domésticos com esporotricose atendidos no IPEC/FIOCRUZ com ênfase na infecção por Bartonella spp

Made available in DSpace on 2016-01-07T13:34:26Z (GMT). No. of bitstreams: 2 amanda_kitada_ipec_mest_2013.pdf: 1152590 bytes, checksum: 4fb3c0cb040e31e0933902d7ef3dd156 (MD5) license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5) Previous issue date: 2015-10-29Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, BrasilA doença da arranhadura do gato é uma zoonose causada por bactérias do gênero Bartonella. O gato atua como reservatório de Bartonella henselae e a transmissão ao humano ocorre através da arranhadura ou mordedura. A esporotricose, causada por fungos do complexo Sporothrix, é transmitida aos humanos através da implantação traumática deste microrganismo no tecido subcutâneo. Os gatos com esporotricose apresentam lesões cutâneas ulceradas com elevada carga parasitária e têm importante papel na transmissão. Nos últimos 14 anos foram diagnosticados mais de 3.000 casos de esporotricose felina no IPEC/FIOCRUZ. Com o objetivo de estudar a soroprevalência de infecção por Bartonella spp. em gatos com esporotricose, 112 amostras de soro foram submetidas ao teste de imunofluorescência indireta utilizando o kit B. henselae IFA IgG (Bion®, USA). Além disso, foi realizada a pesquisa de anticorpos anti-vírus da leucemia felina (FeLV) e antígenos do vírus da imunodeficiência felina (FIV) utilizando kit comercial Snap Combo FIV-FeLV (Idexx®, USA). Um grupo composto por 77 amostras de soro de gatos sem lesões cutâneas aparentes também foi incluído no estudo. No grupo de gatos com esporotricose, 93 eram machos, a idade mediana foi 22 meses e oito (7,1%) foram positivos para FIV e 15 (13,4%) para FeLV No grupo sem lesões cutâneas, 36 eram machos, a idade mediana foi 48 meses, e dez (13,0%) gatos foram positivos para FIV e oito (10,4%) para FeLV. Dos 112 gatos com esporotricose e dos 77 sem leões cutâneas, 72 (64,3%) e 35 (45,5%), respectivamente, foram reativos ao teste de imunofluorescência para Bartonella spp. Não houve associação entre as variáveis faixa etária, sexo, status sorológico para FIV/FeLV e a presença de anticorpos anti-Bartonella spp. Os resultados obtidos sugerem que a população de gatos com esporotricose deste estudo pode ser considerada uma potencial fonte de infecção humana também para Bartonella sppCat scratch dise ase is a zoonosis caused by species of genus Bartonella . Cats are the main reservoir of Bartonella henselae . Transmission of these bacteria to humans occurs through bites or scratches of infected cats. Sporotrichosis, caused by fungus of Sporothrix complex , is transmitted by traumatic inoculation of soil, plants and organic matter contaminated with the fungus. Cats are important in zoonotic transmission because of the large amount of yeast cells in the lesions. In the last 14 years were diagnosed more than 3.000 cases of feline sporotrichosis in IPEC/FIOCRUZ. The main objective of this study was to investigate the prevalence of infection by Bartonella spp. in cats with sporotrichosis. Serum samples from 112 domestic cats were analyzed by indirect immunofluor escence test assay (IFA) using the commercial kit B. henselae IFA IgG (Bion®, USA). In addition, it was detected the presence of antibodies to feline leukemia vírus (FeLV) and antigens of feline immunodeficiency virus (FIV) using the commercial kit Snap Co mbo FIV - FeLV (Idexx®, USA). One group of 77 serum samples from cats with no apparent skin lesions was also included in the study. In the group of animals with sporotrichosis, 93 were males, median age was 22 months, and eight (7. 1% ) were positive for FIV, 15 (13. 4%) were positive for FeLV . In the group of animals without skin lesions 36 were males, median age was 48 months, and ten (13. 0%) w ere positive for FIV, eight (10. 4%) were positive for FeLV . Of the 112 cats with sporotrichosis and 77 ca ts without sk in lesions, 72 (64.3%) e 35 (45. 5%), respectively, were reactive to IFA . There was no association between age, sex, FIV/FeLV and the presence of antibodies to Bartonella spp. The results suggest that the study population can be considered a potential sourc e of human infection by both zoonos

The epidemiological scenario of feline sporotrichosis in Rio de Janeiro, State of Rio de Janeiro, Brazil

Introduction Sporotrichosis is a mycosis affecting both humans and animals. Within the context of the ongoing sporotrichosis epidemic in the State of Rio de Janeiro, Brazil, sick cats plays an important role in the zoonotic transmission. The aim of this study was to update the number of feline cases diagnosed at the Fundação Oswaldo Cruz (2005-2011). Methods The medical records of the cats followed were reviewed; the inclusion criterion was the isolation of Sporothrix spp. in culture. Results In total, 2,301 feline cases were identified. Conclusions These results should alert sanitary authorities to the difficulties associated with sporotrichosis control

Identificação molecular de bartonella henselae em paciente com SIDA soronegativo para doença da arranhadura do gato no Rio de Janeiro, Brasil

Submitted by Rodrigo Senorans ([email protected]) on 2015-04-22T16:43:23Z No. of bitstreams: 1 MOLECULAR IDENTIFICATION OF Bartonella henselae IN A SERONEGATIVE CAT SCRATCH DISEASE PATIENT WITH AIDS IN RIO DE JANEIRO, BRAZIL.pdf: 336144 bytes, checksum: 32d4441f4678489c606525e767614d6a (MD5)Approved for entry into archive by Anderson Silva ([email protected]) on 2015-04-27T16:06:18Z (GMT) No. of bitstreams: 1 MOLECULAR IDENTIFICATION OF Bartonella henselae IN A SERONEGATIVE CAT SCRATCH DISEASE PATIENT WITH AIDS IN RIO DE JANEIRO, BRAZIL.pdf: 336144 bytes, checksum: 32d4441f4678489c606525e767614d6a (MD5)Approved for entry into archive by Anderson Silva ([email protected]) on 2015-04-29T16:41:16Z (GMT) No. of bitstreams: 1 MOLECULAR IDENTIFICATION OF Bartonella henselae IN A SERONEGATIVE CAT SCRATCH DISEASE PATIENT WITH AIDS IN RIO DE JANEIRO, BRAZIL.pdf: 336144 bytes, checksum: 32d4441f4678489c606525e767614d6a (MD5)Made available in DSpace on 2015-05-08T16:14:05Z (GMT). No. of bitstreams: 1 MOLECULAR IDENTIFICATION OF Bartonella henselae IN A SERONEGATIVE CAT SCRATCH DISEASE PATIENT WITH AIDS IN RIO DE JANEIRO, BRAZIL.pdf: 336144 bytes, checksum: 32d4441f4678489c606525e767614d6a (MD5) Previous issue date: 2014Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hantaviroses e Rickettsioses. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hantaviroses e Rickettsioses. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hantaviroses e Rickettsioses. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hantaviroses e Rickettsioses. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hantaviroses e Rickettsioses. Rio de Janeiro, RJ, BrasilBartonella henselae está associada a um amplo espectro de manifestações clínicas, incluindo a doença da arranhadura de gato, endocardite, e meningoencefalite, em pacientes imunocompetentes e imunocomprometidos. Relatamos o primeiro caso confirmado por método molecular de B. henselae em um paciente com SIDA no estado do Rio de Janeiro, Brasil. Apesar da sequência de DNA de B. henselae ser detectada pela reação em cadeia da polimerase em uma biópsia do linfonodo, soros das fases aguda e convalescente foram não reativos.Bartonella henselae is associated with a wide spectrum of clinical manifestations, including cat scratch disease, endocarditis and meningoencephalitis, in immunocompetent and immunocompromised patients. We report the first molecularly confirmed case of B. henselae infection in an AIDS patient in state of Rio de Janeiro, Brazil. Although DNA sequence of B. henselae has been detected by polymerase chain reaction in a lymph node biopsy, acute and convalescent sera were nonreactive

Feline Sporotrichosis: Coinfection with Toxoplasma gondii, Feline Immunodeficiency Virus and Feline Leukemia Virus in Cats From an Endemic Area in Brazil

Submitted by sandra infurna ([email protected]) on 2016-03-29T12:26:09Z No. of bitstreams: 1 renata_barros_etal_IOC_2015.pdf: 242351 bytes, checksum: 5293d7b28a79664a3e6f8de628c17e2b (MD5)Approved for entry into archive by sandra infurna ([email protected]) on 2016-03-29T12:46:34Z (GMT) No. of bitstreams: 1 renata_barros_etal_IOC_2015.pdf: 242351 bytes, checksum: 5293d7b28a79664a3e6f8de628c17e2b (MD5)Made available in DSpace on 2016-03-29T12:46:34Z (GMT). No. of bitstreams: 1 renata_barros_etal_IOC_2015.pdf: 242351 bytes, checksum: 5293d7b28a79664a3e6f8de628c17e2b (MD5) Previous issue date: 2015Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxoplasmose. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos (LAPCLIN-DERMZOO). Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos (LAPCLIN-DERMZOO). Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos (LAPCLIN-DERMZOO). Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos (LAPCLIN-DERMZOO). Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Epidemiologia Clínica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxoplasmose. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxoplasmose. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxoplasmose. Rio de Janeiro, RJ, Brasil / Universidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia e Parasitologia. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos (LAPCLIN-DERMZOO). Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxoplasmose. Rio de Janeiro, RJ, Brasil.Background: Sporotrichosis is an endemic zoonosis in the metropolitan region of Rio de Janeiro caused by fungi included in the Sporothrix complex, in which cats are the main source of infection for humans and animals. Coinfections in cats with sporotrichosis from this region, their risk factors and how they affect the treatment outcome in these animals are little known. The objectives of this study were to determine the coinfections of Sporothrix spp. with Toxoplasma gondii, Feline Immunodeficiency Virus (FIV) and Feline Leukemia Virus (FeLV) and to correlate these infections with risk factors and the outcome of sporotrichosis treatment in cats from an endemic area of sporotrichosis in Rio de Janeiro, Brazil. Materials, Methods & Results: It was conducted a cohort study involving 213 cats with definitive diagnosis of sporotrichosis from the metropolitan area of Rio de Janeiro and assisted in the Laboratory of Clinical Research on Dermatozoonosis in Domestic Animals (LAPCLIN-DERMZOO)/Evandro Chagas National Institute of Infectious Diseases (INI)/Oswaldo Cruz Foundation (Fiocruz), in Rio de Janeiro, RJ, Brazil, from November 2007 until February 2011. These animals were monthly evaluated due to sporotrichosis treatment until their sporotrichosis treatment outcomes. In every clinical evaluation, 5 mL of blood were collected in order to obtain the serum, which was stored at -20ºC. Information from the animal’s medical records have also been collected, such as sex, eating habits, living with other cats, access to the streets, castration, age and the outcome of sporotrichosis treatment. Serological follow-up of anti-T. gondii antibodies were performed through indirect hemagglutination assay (IHA) and indirect fluorescent antibody test (IFAT) in all clinical evaluations. The FIV and FeLV antibody detection were made through a rapid immunoassay using the cats’serum samples from the first clinical evaluation. Fisher’s exact test was applied to verify associations between T. gondii, FIV and FeLV coinfections, the outcome of sporotrichosis treatment and risk factors. To compare IHA and IFAT, the values of total, positive and negative concordances were evaluated. A P-value < 0.05 indicated significant associations in the statistical tests. Of the 213 cats, fourteen (6.6%) showed antibodies anti-T. gondii, twelve (5.6%) anti-FIV and thirty-five (16.4%) anti-FeLV. There was a concordance of 100% between IFAT and IHA for the serological diagnosis of T. gondii infection. No statistical difference was observed between the presence of anti-T gondii antibodies with the FIV and FeLV infections and with the outcome of sporotrichosis treatment (P > 0.05). Furthermore there was no significant statistical difference between the presence of anti-T gondii antibodies and the variables sex, eating habits, living with other cats, free access to the street, castration and age (P > 0.05). The follow-up of anti-T.gondii antibodies showed that in two cats there was a fourfold rise in the titers between two consecutive follow-ups and in one there was seroconversion, which were indicative of acute infection. Discussion: The occurrence of coinfections of sporotrichosis with T. gondii, FIV and FeLV was low in cats from the metropolitan region of Rio de Janeiro, Brazil, where sporotrichosis is endemic. This was the first study that determine and follow-up the frequency of anti- T. gondii antibodies in a group of cats diagnosed with sporotrichosis. The fact that cats were domiciled with adequate feeding and management, the low frequency of T. gondii and the rare cases indicative of acute infection in the study population indicate that these animals are not highly exposed to infection by this protozoan