Primeiro registro de epidemias causadas pelo vírus Oropouche nos Estados do Maranhão e Goiás, Brasil

The authors describe the occurrence of outbreaks caused by Oropouche virus (ORO) in the states of Maranhão and Goiás, Brazil in 1988. 36 strains of the virus were obtained from the intracerebral inoculation of the blood of 120 patients into 2-3 day-old infant mice. The illness was characterized by headache, fever, pain in the muscles, joints and back, photophobia, retrobulbar pain, nausea and dizziness. 128 of 197 people examined in Porto Franco, MA, had hemagglutination-inhibiting antibodies to the agent, while 106 of them had IgM antibodies by MAC ELISA test. All age groups were infected, although the incidence was higher among who had 10 to 19 years old. There was no difference, in relation to sex infections. Recurrence of symptoms was reported in 56% of sick people. Mice inoculated with 3624 Culicoides paraensis (Ceratopogonidae) and 1970 Culex (Cux.) quinquefasciatus (Culicidae) collected in Porto Franco resulted in one single isolation of ORO virus, from the Culicoides. These are the first confirmed cases of ORO infection in Maranhão and Goiás states.Os autores descrevem a ocorrência de epidemias causadas pelo vírus Oropouche (ORO) nos Estados do Maranhão (MA) e Goiás (GO) em 1988. 36 amostras de vírus foram obtidas a partir da inoculação do sangue de 120 pacientes em camundongos recém nascidos. A doença foi caracterizada por febre, cefaléia, dores musculares, articulares, fotofobia, dor retro ocular, náuseas e tontura. 128 das 197 pessoas examinadas em Porto Franco, MA, tinham anticorpos inibidores da hemaglutinação (IH) para o agente e, em 106 foram detectados anticorpos IgM por MAC ELISA. Todos os grupos etários foram infectados, embora a incidência tenha sido mais elevada entre aqueles com 10 a 19 anos de idade. Quanto ao sexo, a infecção ocorreu igualmente em ambos os sexos. Recorrência dos sintomas foi observada em 56% dos casos positivos estudados. A inoculação em camundongos Swiss recém nascidos de 3.624 Culicoides paraensis (Ceratopogonidae) e 1.970 Culex (Culex) quinquefasciatus (Culicidae), coletados em Porto Franco-MA, resultou em um único isolamento do vírus ORO a partir dos Culicoides. Essa é a primeira descrição de casos confirmados de infecção pelo vírus Oropouche nos Estados do Maranhão e Goiás, Brasil

Characterization of the Gamboa virus serogroup (Orthobunyavirus genus, Peribunyaviridae family)

Comprehensive comparative phylogenetic analyses were performed on 17 Gamboa serogroup viruses (GAMSVs) from distinct geographic regions in the Americas and other representative members of the genus Orthobunyavirus (Peribunyaviridae), based on small (S), medium (M), and large (L) open reading frame full-length and partial sequences. Genome characterization showed that the GAMSVs divide into four clades or genotypes. The GAMSVs have a genetic organization similar to other orthobunyaviruses, except that they have a larger NSm protein than other orthobunyaviruses. A serosurvey for Gamboa virus antibodies was performed in plasma from birds, other wild animals, and humans living around the Tucuruí hydroelectric dam in Pará state, northern Brazil, a known focus of GAMSV activity. Newborn chicks (Gallus gallus domesticus) were experimentally infected with a GAMSV, and the pathogenesis is described. Histopathological changes were primarily in the lungs and liver. Also, a review of the ecology of the GAMSVs in the Americas is included. In sum, this study presents the genomic and evolutionary characterization of the Gamboa group and the potential model of pathogenesis, which would be helpful for diagnostic purposes, epidemiology, and immunopathogenesis studies

Arboviroses in the Brazilian Amazônia

Ministério da Saúde. Fundação Serviço de Saúde Pública. Instituto Evandro Chagas. Belém, PA, Brasil

A seroepidemiological study of previously unreported Alpha and Bunyaviruses in Nigerian human and animal populations

University of Ibadan. College of Medicine. Departament of Virology. Ibadan, Oyo state, Nigeria.Ministry of Health. Public Health Services Foundation. Evandro Chagas Institute. Belém, PA, Brazil

Simultaneous infections of St.Louis encephalitis virus and Leptospira in A patient from Belem, Para, Brazil, 1978

Ministério da Saúde. Fundação Serviços de Saúde Pública. Instituto Evandro Chagas. Belém, PA, Brasil.Ministério da Saúde. Fundação Serviços de Saúde Pública. Instituto Evandro Chagas. Belém, PA, Brasil

Urucuri (Uru) Strain: Be An 100049

Ministério da Saúde. Fundação Serviços de Saúde Pública. Instituto Evandro Chagas. Belém, PA, Brasil.Ministério da Saúde. Fundação Serviços de Saúde Pública. Instituto Evandro Chagas. Belém, PA, Brasil.Antigenic group: Phlebotomus fever (PHL). Original source: Isolated by Belem Virus Laboratory from whole blood of apparently well young male Proechimys guyannensis 1 collected on 19 April 1966 in Utinga forest, Lat. 1°28'S,Long. 48°27'W. Habitat: Floor of watershed forest. Storage: Frozen at -60°C. Method of isolation in laboratory: Blood inoculated ic into infant mice on 20 April 1966. Validity of isolation: Reisolation not tried and presence of homologous antibody in source animal not tested. However, four more strains isolated from Proechimys guyannensis captured in the same area. Properties of virus: Physical: No data. Chemical: No data. Resistance to chemicals: Sensitive to 1:2 ether; control titer 103.8, after-treatment titer 101.3. Sensitive to 1:1,000 sodium deoxycholate; control titer 105.0, after-treatment titer 101.0. Antigenic: Hemagglutinin detected using infected suckling mouse brains extracted with sucrose-acetone, treated with protamine sulfate, and tested with goose erythrocytes at pH 5.8 through 6.2 and at room temperature. Optimum pH was 6.0

Antigenic relationship among rhabdoviruses infecting terrestrial vertebrates

Yale Universyt. School of Medicine. Department of Epidemiology and Public Health. Yale Arbovirus Research Unit. New Haven, CO, U.S.A.Ministério da Saúde. Fundação Serviços de Saúde Pública. Instituto Evandro Chagas. Belém, PA, Brasil.Ministério da Saúde. Fundação Serviços de Saúde Pública. Instituto Evandro Chagas. Belém, PA, Brasil.Serological studies (complement-fixation, immunofluorescent and plaque reduction neutralization) were carried out with 50 vertebrate rhabdoviruses to determine their antigenic relationships. On the basis of these studies, an antigenic classification ofrhabdoviruses infecting terrestrial vertebrates is proposed. This classification includesthree major and four minor serogroups. Several new members of the vesicular stomatitis serogroup were identified

Estudos sobre arbovírus nas áreas de Cachoeira Porteira e Porto Trombetas, Oriximiná, Estado do Pará - 1978-1980

Ministério da Saúde. Fundação Serviços de Saúde Pública. Instituto Evandro Chagas. Belém, PA, Brasil.Ministério da Saúde. Fundação Serviços de Saúde Pública. Instituto Evandro Chagas. Belém, PA, Brasil.Ministério da Saúde. Fundação Serviços de Saúde Pública. Instituto Evandro Chagas. Belém, PA, Brasil