Locomotor behaviour of Blattella germanica modified by DEET

N,N-diethyl-3-methylbenzamide (DEET) is the active principle of most insect repellents used worldwide. However, its toxicity on insects has not been widely studied. The aim of this work is to study the effects of DEET on the locomotor activity of Blattella germanica . DEET has a dose-dependent repellent activity on B. germanica. Locomotor activity was significantly lower when insects were pre-exposed to 700 μg/cm2 of DEET for 20 or 30 minutes, but it did not change when pre-exposure was shorter. Locomotor activity of insects that were pre-exposed to 2.000 μg/cm2 of DEET for 10 minutes was significantly lower than the movement registered in controls. No differences were observed when insects were pre-exposed to lower concentrations of DEET. A 30-minute pre-exposure to 700 μg/cm2 of DEET caused a significant decrease in locomotor activity. Movement was totally recovered 24 h later. The locomotor activity measured during the exposure to different concentrations of DEET remained unchanged. Insects with decreased locomotor activity were repelled to the same extent than control insects by the same concentration of DEET. We demonstrated that the repellency and modification of locomotor activity elicited by DEET are non-associated phenomena. We also suggested that the reduction in locomotor activity indicates toxicity of DEET, probably to insect nervous system.Fil: Sfara, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; Argentina. Universidad Nacional de San Martín. Instituto de Investigación en Ingeniería Ambiental; ArgentinaFil: Mougabure Cueto, Gastón Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; ArgentinaFil: Zerba, Eduardo Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; Argentina. Universidad Nacional de San Martín. Instituto de Investigación en Ingeniería Ambiental; ArgentinaFil: Alzogaray, Raul Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; Argentina. Universidad Nacional de San Martín. Instituto de Investigación en Ingeniería Ambiental; Argentin

Primer registro de Chrysoperla asoralis y C. argentina (Neuroptera: Chrysopidae) en cultivos hortícolas de La Plata asociado a pimiento (Capsicum annuum L.)

Chrisopids are economically significant predators of several agricultural pests. In Argentina, the species recorded of Chrysoperla Steinmann, 1964 are C. externa (Hagen, 1861), C. asoralis (Banks, 1915), C. argentina (González Olazo & Reguilón, 2002) and C. defreitasi (Brooks, 1994). Chrysoperla asoralis and C. argentina have been associated with citric, olive, maize and cotton crops. In the present note, we record for the first time these two species in La Plata and neighboring areas within the Buenos Aires province, in association with the sweet pepper, Capsicum annuum L.Los crisópidos son depredadores, económicamente importantes, de varias plagas de la agricultura. En Argentina, las especies registradas dentro del género Chrysoperla Steinmann, 1964 son C. externa (Hagen, 1861), C. asoralis (Banks, 1915), C. argentina (González Olazo & Reguilón, 2002) y C. defreitasi (Brooks, 1994). Chrysoperla argentina y C. asoralis han sido citadas asociadas con cultivos de cítricos, olivos, maíz y algodón. En la presente nota registramos, por primera vez, estas dos especies en la zona del Gran La Plata, Provincia de Buenos Aires, en asociación con el pimiento, Capsicum annuum L.Centro de Estudios Parasitológicos y de VectoresFacultad de Ciencias Naturales y Muse

Primer registro de Chrysoperla asoralis y C. argentina (Neuroptera: Chrysopidae) en cultivos hortícolas de La Plata asociado a pimiento (Capsicum annuum L.)

Chrisopids are economically significant predators of several agricultural pests. In Argentina, the species recorded of Chrysoperla Steinmann, 1964 are C. externa (Hagen, 1861), C. asoralis (Banks, 1915), C. argentina (González Olazo & Reguilón, 2002) and C. defreitasi (Brooks, 1994). Chrysoperla asoralis and C. argentina have been associated with citric, olive, maize and cotton crops. In the present note, we record for the first time these two species in La Plata and neighboring areas within the Buenos Aires province, in association with the sweet pepper, Capsicum annuum L.os crisópidos son depredadores, económicamente importantes, de varias plagas de la agricultura. En Argentina, las especies registradas dentro del género Chrysoperla Steinmann, 1964 son C. externa (Hagen, 1861), C. asoralis (Banks, 1915), C. argentina (González Olazo & Reguilón, 2002) y C. defreitasi (Brooks, 1994). Chrysoperla argentina y C. asoralis han sido citadas asociadas con cultivos de cítricos, olivos, maíz y algodón. En la presente nota registramos, por primera vez, estas dos especies en la zona del Gran La Plata, Provincia de Buenos Aires, en asociación con el pimiento, Capsicum annuum L.Fil: Haramboure, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Estudios Parasitológicos y de Vectores (i); ArgentinaFil: Reguilón, Carmen. Fundación Miguel Lillo; ArgentinaFil: Alzogaray, Raul Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas Para la Defensa. Centro de Investigación de Plagas E Insecticidas; Argentina. Universidad Nacional de San Martín; ArgentinaFil: Schneider, Marcela Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Estudios Parasitológicos y de Vectores (i); Argentina. Universidad Nacional de La Plata; Argentin

Primer registro de Chrysoperla asoralis y C. argentina (Neuroptera: Chrysopidae) en cultivos hortícolas de La Plata asociado a pimiento (Capsicum annuum L.)

Chrisopids are economically significant predators of several agricultural pests. In Argentina, the species recorded of Chrysoperla Steinmann, 1964 are C. externa (Hagen, 1861), C. asoralis (Banks, 1915), C. argentina (González Olazo & Reguilón, 2002) and C. defreitasi (Brooks, 1994). Chrysoperla asoralis and C. argentina have been associated with citric, olive, maize and cotton crops. In the present note, we record for the first time these two species in La Plata and neighboring areas within the Buenos Aires province, in association with the sweet pepper, Capsicum annuum L.Los crisópidos son depredadores, económicamente importantes, de varias plagas de la agricultura. En Argentina, las especies registradas dentro del género Chrysoperla Steinmann, 1964 son C. externa (Hagen, 1861), C. asoralis (Banks, 1915), C. argentina (González Olazo & Reguilón, 2002) y C. defreitasi (Brooks, 1994). Chrysoperla argentina y C. asoralis han sido citadas asociadas con cultivos de cítricos, olivos, maíz y algodón. En la presente nota registramos, por primera vez, estas dos especies en la zona del Gran La Plata, Provincia de Buenos Aires, en asociación con el pimiento, Capsicum annuum L.Centro de Estudios Parasitológicos y de VectoresFacultad de Ciencias Naturales y Muse

Susceptibility of different life stages of Blattella germanica (Blattodea: Blattellidae) and Periplaneta fuliginosa (Blattodea: Blattidae) to entomopathogenic fungi

The susceptibility of nymphs and adults of the German cockroach, Blattella germanica Linnaeus (Blattodea: Blattellidae) and the smokybrown cockroach, Periplaneta fuliginosa Serville (Blattodea: Blattidae) to Argentinian isolates of the entomopathogenic fungi Metarhizium anisopliae (Metschn.) Sorokin (CEP 085) and Beauveria bassiana (Bals.-Criv.) Vuill. (CEP 077) was evaluated. Fungi were tested by using two different methods: bait and direct contact. Mortality was monitored daily for twenty days to obtain LT50. M. anisopliae produced 60 and 93% mortality in nymphs and adults of B. germanica, respectively, when conidia were applied by direct contact. The LT50 for adults was 3.8 days, and 8.6 days for nymphs. Direct contact of B. bassiana produced 80% mortality on adults of B. germanica with a LT50 of 4.9 days, and for nymphs 40 % mortality in 10 days. When B. germanica was exposed to bait, the level of mortality was significant in adults. Nymphs of P. fuliginosa were treated with bait with M. anisopliae and B. bassiana and they caused 50% mortality with a LT50 of 22 days, and LT50 of 27 days respectively. Nymphs and adults of P. fuliginosa treated by direct contact and adults treated with bait showed that mortality level was not significantly different as compared to the control. Results showed differences in susceptibility between the two species of cockroaches and between nymphs and adults of the same species. In addition, different responses to the fungal species with the two methods that were used in the bioassays have been demonstrated. This is the first report of susceptibility of P. fuliginosa to entomopathogenic fungi. This study demonstrates the potential of fungi as biocontrol agents against this pest.Fil: Gutierrez, Alejandra Concepción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Estudios Parasitológicos y de Vectores (i); Argentina. Universidad Nacional de La Plata; ArgentinaFil: García, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Estudios Parasitológicos y de Vectores (i); Argentina. Universidad Nacional de La Plata; ArgentinaFil: Alzogaray, Raul Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; ArgentinaFil: Urrutia, María I.. Universidad Nacional de La Plata; ArgentinaFil: Lopez Lastra, Claudia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Estudios Parasitológicos y de Vectores (i); Argentina. Universidad Nacional de La Plata; Argentin

Susceptibility of different life stages of <i>Blattella germanica</i> (Blattodea: Blattellidae) and <i>Periplaneta fuliginosa</i> (Blattodea: Blattidae) to entomopathogenic fungi

The susceptibility of nymphs and adults of the German cockroach, Blattella germanica Linnaeus (Blattodea: Blattellidae) and the smokybrown cockroach, Periplaneta fuliginosa Serville (Blattodea: Blattidae) to Argentinian isolates of the entomopathogenic fungi Metarhizium anisopliae (Metschn.) Sorokin (CEP 085) and Beauveria bassiana (Bals.-Criv.) Vuill. (CEP 077) was evaluated. Fungi were tested by using two different methods: bait and direct contact. Mortality was monitored daily for twenty days to obtain LT50. M. anisopliae produced 60 and 93% mortality in nymphs and adults of B. germanica, respectively, when conidia were applied by direct contact. The LT50 for adults was 3.8 days, and 8.6 days for nymphs. Direct contact of B. bassiana produced 80% mortality on adults of B. germanica with a LT50 of 4.9 days, and for nymphs 40 % mortality in 10 days. When B. germanica was exposed to bait, the level of mortality was significant in adults. Nymphs of P. fuliginosa were treated with bait with M. anisopliae and B. bassiana and they caused 50% mortality with a LT50 of 22 days, and LT50 of 27 days respectively. Nymphs and adults of P. fuliginosa treated by direct contact and adults treated with bait showed that mortality level was not significantly different as compared to the control. Results showed differences in susceptibility between the two species of cockroaches and between nymphs and adults of the same species. In addition, different responses to the fungal species with the two methods that were used in the bioassays have been demonstrated. This is the first report of susceptibility of P. fuliginosa to entomopathogenic fungi. This study demonstrates the potential of fungi as biocontrol agents against this pest.Centro de Estudios Parasitológicos y de Vectore

Susceptibility of different life stages of <i>Blattella germanica</i> (Blattodea: Blattellidae) and <i>Periplaneta fuliginosa</i> (Blattodea: Blattidae) to entomopathogenic fungi

The susceptibility of nymphs and adults of the German cockroach, Blattella germanica Linnaeus (Blattodea: Blattellidae) and the smokybrown cockroach, Periplaneta fuliginosa Serville (Blattodea: Blattidae) to Argentinian isolates of the entomopathogenic fungi Metarhizium anisopliae (Metschn.) Sorokin (CEP 085) and Beauveria bassiana (Bals.-Criv.) Vuill. (CEP 077) was evaluated. Fungi were tested by using two different methods: bait and direct contact. Mortality was monitored daily for twenty days to obtain LT50. M. anisopliae produced 60 and 93% mortality in nymphs and adults of B. germanica, respectively, when conidia were applied by direct contact. The LT50 for adults was 3.8 days, and 8.6 days for nymphs. Direct contact of B. bassiana produced 80% mortality on adults of B. germanica with a LT50 of 4.9 days, and for nymphs 40 % mortality in 10 days. When B. germanica was exposed to bait, the level of mortality was significant in adults. Nymphs of P. fuliginosa were treated with bait with M. anisopliae and B. bassiana and they caused 50% mortality with a LT50 of 22 days, and LT50 of 27 days respectively. Nymphs and adults of P. fuliginosa treated by direct contact and adults treated with bait showed that mortality level was not significantly different as compared to the control. Results showed differences in susceptibility between the two species of cockroaches and between nymphs and adults of the same species. In addition, different responses to the fungal species with the two methods that were used in the bioassays have been demonstrated. This is the first report of susceptibility of P. fuliginosa to entomopathogenic fungi. This study demonstrates the potential of fungi as biocontrol agents against this pest.Centro de Estudios Parasitológicos y de Vectore

Susceptibility of different life stages of <i>Blattella germanica</i> (Blattodea: Blattellidae) and <i>Periplaneta fuliginosa</i> (Blattodea: Blattidae) to entomopathogenic fungi

The susceptibility of nymphs and adults of the German cockroach, Blattella germanica Linnaeus (Blattodea: Blattellidae) and the smokybrown cockroach, Periplaneta fuliginosa Serville (Blattodea: Blattidae) to Argentinian isolates of the entomopathogenic fungi Metarhizium anisopliae (Metschn.) Sorokin (CEP 085) and Beauveria bassiana (Bals.-Criv.) Vuill. (CEP 077) was evaluated. Fungi were tested by using two different methods: bait and direct contact. Mortality was monitored daily for twenty days to obtain LT50. M. anisopliae produced 60 and 93% mortality in nymphs and adults of B. germanica, respectively, when conidia were applied by direct contact. The LT50 for adults was 3.8 days, and 8.6 days for nymphs. Direct contact of B. bassiana produced 80% mortality on adults of B. germanica with a LT50 of 4.9 days, and for nymphs 40 % mortality in 10 days. When B. germanica was exposed to bait, the level of mortality was significant in adults. Nymphs of P. fuliginosa were treated with bait with M. anisopliae and B. bassiana and they caused 50% mortality with a LT50 of 22 days, and LT50 of 27 days respectively. Nymphs and adults of P. fuliginosa treated by direct contact and adults treated with bait showed that mortality level was not significantly different as compared to the control. Results showed differences in susceptibility between the two species of cockroaches and between nymphs and adults of the same species. In addition, different responses to the fungal species with the two methods that were used in the bioassays have been demonstrated. This is the first report of susceptibility of P. fuliginosa to entomopathogenic fungi. This study demonstrates the potential of fungi as biocontrol agents against this pest.Centro de Estudios Parasitológicos y de Vectore