Preservation of fertility: the importance of providing this possibility to patients with cancer

Universidade Federal de São Paulo (UNIFESP) Departamento de GinecologiaUNIFESP, Depto. de GinecologiaSciEL

Evaluation of FAS and caspase-3 in the endometrial tissue of patients with idiopathic infertility and recurrent pregnancy loss

Objective: To investigate expression of BcL-2, FAS, FAS ligand (FASL) and cleaved caspase-3 in the endometrial tissue of women with idiopathic infertility (with two consecutive failed cycles of in vitro fertilization) and women with idiopathic recurrent pregnancy loss. the control group consisted of fertile women.Study design: Endometrial tissue samples from fertile women (n = 25), women with idiopathic infertility (n = 25) and women with idiopathic recurrent pregnancy loss (n = 25) were collected on the seventh or eighth postovulatory day of their menstrual cycles for evaluation. Expression of BcL-2, FAS, FASL and cleaved caspase-3 was assessed using immunohistochemical methods.Results: Expression of BcL-2 and FAS was significantly higher and lower, respectively, in the women with idiopathic infertility than in the other groups (p < 0.01). Expression of cleaved caspase-3 was significantly lower in the women with idiopathic infertility than in the other groups (p < 0.01). Expression of FASL was similar in all three groups.Conclusion: Disturbances in endometrial apoptosis may be a contributing factor in patients with idiopathic infertility and recurrent pregnancy loss. (C) 2012 Elsevier Ireland Ltd. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Federal de São Paulo, Dept Gynaecol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Obstet, São Paulo, BrazilUniv São Paulo, Dept Obstet & Gynaecol, Gynaecol Div, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Gynaecol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Obstet, São Paulo, BrazilFAPESP: 2004/01424-0Web of Scienc

Successful birth after intrafallopian transfer of microhatched embryos

Objective: To present a successful transfer of microhatched embryos to the fallopian tubes via microlaparoscopy.Design: Case report.Setting: Private practice affiliated with a medical university.Patient: A 40-year-old woman with primary infertility, mildly elevated baseline FSH levels, and a history of poor ovarian response to ovulation induction. Her husband had severe oligoospermia after vasectomy reversal.Intervention(s): Late luteal leuprolide acetate to pituitary down-regulation followed by pure FSH, 300 IU, and hMG, 300 IU, daily for ovulation induction. Transvaginal oocyte retrieval, intracytoplasmic sperm injection, assisted embryo hatching, microlaparoscopic intrafallopian ET.Main Outcome Measure(s): Amniocentesis at the 14th week of gestation revealed a normal karyotype (46,XX); birth of a normal female infant (3700 g).Result(s): Establishment of a single, viable intrauterine gestation followed by a vaginal delivery at term.Conclusion(s): This case shows the possibility of using assisted-hatched embryos for laparoscopic intrafallopian tube transfer. (C) 1997 by American Society for Reproductive Medicine.Universidade Federal de São Paulo,ESCOLA PAULISTA MED,DEPT OBSTET & GYNECOL,São Paulo,BRAZILUniversidade Federal de São Paulo,ESCOLA PAULISTA MED,DEPT OBSTET & GYNECOL,São Paulo,BRAZILWeb of Scienc