Adaptação de um Bioensaio Simplificado para Avaliação do Status de Susceptibilidade em Larvas de Anopheles darlingi e Anopheles marajoara ao Piretroide Deltametrina

Made available in DSpace on 2015-05-19T13:26:06Z (GMT). No. of bitstreams: 2 license.txt: 1914 bytes, checksum: 7d48279ffeed55da8dfe2f8e81f3b81f (MD5) ademir_martinsetal_IOC_2014.pdf: 535113 bytes, checksum: c199881ead9476507b4e632c50a3d1da (MD5) Previous issue date: 2014Instituto Nacional de Pesquisas da Amazônia. Laboratório de Malária e Dengue. Manaus, AM, Brasil.Instituto Nacional de Pesquisas da Amazônia. Laboratório de Malária e Dengue. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Pesquisas da Amazônia. Laboratório de Malária e Dengue. Manaus, AM, Brasil.Instituto Nacional de Pesquisas da Amazônia. Laboratório de Malária e Dengue. Manaus, AM, Brasil.A malária ainda é um dos mais graves problemas de saúde pública do mundo. A ferramenta estratégica mais efetiva para tentar diminuir a transmissão da doença é a eliminação de vetores na forma adulta por meio da aplicação de inseticidas químicos. No entanto, o uso exacerbado de tais compostos tem provocado uma forte pressão de seleção sobre as populações de vetores, resultando na evolução da resistência. Nesse trabalho, objetivamos adaptar um método de avaliação do perfil de susceptibilidade aos inseticidas piretroides sobre larvas de Anopheles darlingi e Anopheles marajoara. Tal método é conhecido como Bioensaio Simplificado de Knockdown. As amostras foram coletadas em Manaus, São Gabriel da Cachoeira e Iranduba, no estado do Amazonas, em Macapá, no Amapá, e em Rio Branco, no Acre. Os bioensaios foram conduzidos usando larvas de 4º estádio. Cada larva foi colocada em copos contendo 20 mL da solução de inseticida e o tempo de knockdown foi observado durante 30 minutos. Com base na população natural mais susceptível (São Gabriel da Cachoeira), as doses diagnósticas obtidas foram 0,1 e 0,4 ppm. Foram observadas perda de susceptibilidade nas populações de Manaus e Iranduba, possivelmente, devido ao efeito de seleção dos inseticidas sobre os alelos de resistência específicos.Malaria remains one of the most serious public health problems in the world. The most effective strategic tool to reducing the transmission of the disease is the elimination of the mosquito vectors, through applying chemical insecticides against the adult form. However, the overuse of such compounds has been causing a strong selection pressure on vector populations, resulting in the emergence of resistance. In this study, we aimed to adapt a method for assessing susceptibility status to pyrethroid insecticides on larvae of Anopheles darlingi and Anopheles marajoara. This method was previously named as Knockdown Simplified Bioassay. The samples were collected in Manaus, São Gabriel da Cachoeira and Iranduba in the state of Amazonas, in Macapa, Amapa, and Rio Branco, Acre. Bioassays were performed using 4th instar larvae. Each larva was placed in cups containing 20 mL of the insecticide solution and the time of knockdown was observed for 30 minutes. Based on natural population most susceptible (São Gabriel da Cachoeira), diagnostic doses obtained were 0.1 and 0.4 ppm. Loss of susceptibility was observed in populations of Manaus and Iranduba, possibly due to selection effect of insecticides on the specific resistance alleles

First Measurement of the Total Inelastic Cross-Section of Positively-Charged Kaons on Argon at Energies Between 5.0 and 7.5 GeV

International audienceProtoDUNE Single-Phase (ProtoDUNE-SP) is a 770-ton liquid argon time projection chamber that operated in a hadron test beam at the CERN Neutrino Platform in 2018. We present a measurement of the total inelastic cross section of charged kaons on argon as a function of kaon energy using 6 and 7 GeV/$c$ beam momentum settings. The flux-weighted average of the extracted inelastic cross section at each beam momentum setting was measured to be 380$\pm$26 mbarns for the 6 GeV/$c$ setting and 379$\pm$35 mbarns for the 7 GeV/$c$ setting

Supernova Pointing Capabilities of DUNE

International audienceThe determination of the direction of a stellar core collapse via its neutrino emission is crucial for the identification of the progenitor for a multimessenger follow-up. A highly effective method of reconstructing supernova directions within the Deep Underground Neutrino Experiment (DUNE) is introduced. The supernova neutrino pointing resolution is studied by simulating and reconstructing electron-neutrino charged-current absorption on $^{40}$Ar and elastic scattering of neutrinos on electrons. Procedures to reconstruct individual interactions, including a newly developed technique called ``brems flipping'', as well as the burst direction from an ensemble of interactions are described. Performance of the burst direction reconstruction is evaluated for supernovae happening at a distance of 10 kpc for a specific supernova burst flux model. The pointing resolution is found to be 3.4 degrees at 68% coverage for a perfect interaction-channel classification and a fiducial mass of 40 kton, and 6.6 degrees for a 10 kton fiducial mass respectively. Assuming a 4% rate of charged-current interactions being misidentified as elastic scattering, DUNE's burst pointing resolution is found to be 4.3 degrees (8.7 degrees) at 68% coverage

Performance of a modular ton-scale pixel-readout liquid argon time projection chamber

International audienceThe Module-0 Demonstrator is a single-phase 600 kg liquid argon time projection chamber operated as a prototype for the DUNE liquid argon near detector. Based on the ArgonCube design concept, Module-0 features a novel 80k-channel pixelated charge readout and advanced high-coverage photon detection system. In this paper, we present an analysis of an eight-day data set consisting of 25 million cosmic ray events collected in the spring of 2021. We use this sample to demonstrate the imaging performance of the charge and light readout systems as well as the signal correlations between the two. We also report argon purity and detector uniformity measurements, and provide comparisons to detector simulations

Performance of a modular ton-scale pixel-readout liquid argon time projection chamber

International audienceThe Module-0 Demonstrator is a single-phase 600 kg liquid argon time projection chamber operated as a prototype for the DUNE liquid argon near detector. Based on the ArgonCube design concept, Module-0 features a novel 80k-channel pixelated charge readout and advanced high-coverage photon detection system. In this paper, we present an analysis of an eight-day data set consisting of 25 million cosmic ray events collected in the spring of 2021. We use this sample to demonstrate the imaging performance of the charge and light readout systems as well as the signal correlations between the two. We also report argon purity and detector uniformity measurements, and provide comparisons to detector simulations

Performance of a modular ton-scale pixel-readout liquid argon time projection chamber

International audienceThe Module-0 Demonstrator is a single-phase 600 kg liquid argon time projection chamber operated as a prototype for the DUNE liquid argon near detector. Based on the ArgonCube design concept, Module-0 features a novel 80k-channel pixelated charge readout and advanced high-coverage photon detection system. In this paper, we present an analysis of an eight-day data set consisting of 25 million cosmic ray events collected in the spring of 2021. We use this sample to demonstrate the imaging performance of the charge and light readout systems as well as the signal correlations between the two. We also report argon purity and detector uniformity measurements, and provide comparisons to detector simulations

First Measurement of the Total Inelastic Cross-Section of Positively-Charged Kaons on Argon at Energies Between 5.0 and 7.5 GeV

International audienceProtoDUNE Single-Phase (ProtoDUNE-SP) is a 770-ton liquid argon time projection chamber that operated in a hadron test beam at the CERN Neutrino Platform in 2018. We present a measurement of the total inelastic cross section of charged kaons on argon as a function of kaon energy using 6 and 7 GeV/$c$ beam momentum settings. The flux-weighted average of the extracted inelastic cross section at each beam momentum setting was measured to be 380$\pm$26 mbarns for the 6 GeV/$c$ setting and 379$\pm$35 mbarns for the 7 GeV/$c$ setting

The hypothetical track-length fitting algorithm for energy measurement in liquid argon TPCs

International audienceThis paper introduces the hypothetical track-length fitting algorithm, a novel method for measuring the kinetic energies of ionizing particles in liquid argon time projection chambers (LArTPCs). The algorithm finds the most probable offset in track length for a track-like object by comparing the measured ionization density as a function of position with a theoretical prediction of the energy loss as a function of the energy, including models of electron recombination and detector response. The algorithm can be used to measure the energies of particles that interact before they stop, such as charged pions that are absorbed by argon nuclei. The algorithm's energy measurement resolutions and fractional biases are presented as functions of particle kinetic energy and number of track hits using samples of stopping secondary charged pions in data collected by the ProtoDUNE-SP detector, and also in a detailed simulation. Additional studies describe impact of the dE/dx model on energy measurement performance. The method described in this paper to characterize the energy measurement performance can be repeated in any LArTPC experiment using stopping secondary charged pions

Supernova Pointing Capabilities of DUNE

International audienceThe determination of the direction of a stellar core collapse via its neutrino emission is crucial for the identification of the progenitor for a multimessenger follow-up. A highly effective method of reconstructing supernova directions within the Deep Underground Neutrino Experiment (DUNE) is introduced. The supernova neutrino pointing resolution is studied by simulating and reconstructing electron-neutrino charged-current absorption on $^{40}$Ar and elastic scattering of neutrinos on electrons. Procedures to reconstruct individual interactions, including a newly developed technique called ``brems flipping'', as well as the burst direction from an ensemble of interactions are described. Performance of the burst direction reconstruction is evaluated for supernovae happening at a distance of 10 kpc for a specific supernova burst flux model. The pointing resolution is found to be 3.4 degrees at 68% coverage for a perfect interaction-channel classification and a fiducial mass of 40 kton, and 6.6 degrees for a 10 kton fiducial mass respectively. Assuming a 4% rate of charged-current interactions being misidentified as elastic scattering, DUNE's burst pointing resolution is found to be 4.3 degrees (8.7 degrees) at 68% coverage

Performance of a modular ton-scale pixel-readout liquid argon time projection chamber

International audienceThe Module-0 Demonstrator is a single-phase 600 kg liquid argon time projection chamber operated as a prototype for the DUNE liquid argon near detector. Based on the ArgonCube design concept, Module-0 features a novel 80k-channel pixelated charge readout and advanced high-coverage photon detection system. In this paper, we present an analysis of an eight-day data set consisting of 25 million cosmic ray events collected in the spring of 2021. We use this sample to demonstrate the imaging performance of the charge and light readout systems as well as the signal correlations between the two. We also report argon purity and detector uniformity measurements, and provide comparisons to detector simulations