5 research outputs found
Magnetite Mineralization inside Cross-Linked Protein Crystals
Crystallization in confined spaces is a widespread process in nature that also has important implications for the
stability and durability of many man-made materials. It has been reported that confinement can alter essential crystallization events,
such as nucleation and growth and, thus, have an impact on crystal size, polymorphism, morphology, and stability. Therefore, the
study of nucleation in confined spaces can help us understand similar events that occur in nature, such as biomineralization, design
new methods to control crystallization, and expand our knowledge in the field of crystallography. Although the fundamental interest
is clear, basic models at the laboratory scale are scarce mainly due to the difficulty in obtaining well-defined confined spaces allowing
a simultaneous study of the mineralization process outside and inside the cavities. Herein, we have studied magnetite precipitation in
the channels of cross-linked protein crystals (CLPCs) with different channel pore sizes, as a model of crystallization in confined
spaces. Our results show that nucleation of an Fe-rich phase occurs inside the protein channels in all cases, but, by a combination of
chemical and physical effects, the channel diameter of CLPCs exerted a precise control on the size and stability of those Fe-rich
nanoparticles. The small diameters of protein channels restrain the growth of metastable intermediates to around 2 nm and stabilize
them over time. At larger pore diameters, recrystallization of the Fe-rich precursors into more stable phases was observed. This study
highlights the impact that crystallization in confined spaces can have on the physicochemical properties of the resulting crystals and
shows that CLPCs can be interesting substrates to study this process.Ministry of Science and Innovation, Spain (MICINN) PID2020-116261GB-I00
PID2020-118498GB-I00
PDC2021-121135.100FEDER/Junta de Andalucia-Consejeria de Transformacion Economica, Industria, Conocimiento y Universidades (Spain) A-FQM-340-UGR20
UCE-PP2016-0
Synthesis and Biological Activity of Triterpene-Coumarin Conjugates.
A set of 12 maslinic acid-coumarin conjugates was synthesized, with 9 being maslinic acid-diamine-coumarin conjugates at the C-28 carboxylic acid group of triterpene acid and the other three being maslinic acid-coumarin conjugates at C-2/C-3 and/or C-28 of the triterpene skeleton. The cytotoxic effects of these 12 triterpene conjugates were evaluated in three cancer cell lines (B16-F10, HT29, and Hep G2) and compared, respectively, with three nontumor cell lines from the same or similar tissue (HPF, IEC-18, and WRL68). The most potent cytotoxic results were achieved by a conjugate with two molecules of coumarin-3-carboxylic acid coupled through the C-2 and C-3 hydroxy groups of maslinic acid. This conjugate showed submicromolar IC50 values in two of the three cancer cell lines tested (0.6, 1.1, and 0.9 μM), being between 110- and 30-fold more effective than its corresponding precursor. Furthermore, this conjugate (10) showed percentages of cell viability for the three nontumor lines of 90%. Four maslinic acid-coumarin conjugates displayed apoptotic effects in the treated cells, with total apoptosis rates of between 40 and 85%, relative to the control. Almost all the compounds assayed caused cell-cycle arrest in all cancer cell lines, increasing the number of these cells in the G0/G1 phase
International conference on the healthy effect of virgin olive oil
Ageing represents a great concern in developed countries because the number of people involved and the pathologies related with it, like atherosclerosis, morbus Parkinson, Alzheime's disease, vascular dementia, cognitive decline, diabetes and cancer. Epidemiological studies suggest that a Mediterranean diet (which is rich in virgin olive oil) decreases the risk of cardiovascular disease. The Mediterranean diet, rich in virgin olive oil, improves the major risk factors for cardiovascular disease, such as the lipoprotein profile, blood pressure, glucose metabolism and antithrombotic profile. Endothelial function, inflammation and oxidative stress are also positively modulated. Some of these effects are attributed to minor components of virgin olive oil. Therefore, the definition of the Mediterranean diet should include virgin olive oil. Different observational studies conducted in humans have shown that the intake of monounsaturated fat may be protective against age-related cognitive decline and Alzheimer's disease. Microconstituents from virgin olive oil are bioavailable in humans and have shown antioxidant properties and capacity to improve endothelial function. Furthermore they are also able to modify the haemostasis, showing antithrombotic properties. In countries where the populations fulfilled a typical Mediterranean diet, such as Spain, Greece and Italy, where virgin olive oil is the principal source of fat, cancer incidence rates are lower than in northern European countries. The protective effect of virgin olive oil can be most important in the first decades of life, which suggests that the dietetic benefit of virgin olive oil intake should be initiated before puberty, and maintained through life. The more recent studies consistently support that the Mediterranean diet, based in virgin olive oil, is compatible with a healthier ageing and increased longevity. However, despite the significant advances of the recent years, the final proof about the specific mechanisms and contributing role of the different components of virgin olive oil to its beneficial effects requires further investigations. © 2005 Blackwell Publishing Ltd
Magnetite Mineralization inside Cross-Linked Protein Crystals
Crystallization in confined spaces is a widespread process
in nature
that also has important implications for the stability and durability
of many man-made materials. It has been reported that confinement
can alter essential crystallization events, such as nucleation and
growth and, thus, have an impact on crystal size, polymorphism, morphology,
and stability. Therefore, the study of nucleation in confined spaces
can help us understand similar events that occur in nature, such as
biomineralization, design new methods to control crystallization,
and expand our knowledge in the field of crystallography. Although
the fundamental interest is clear, basic models at the laboratory
scale are scarce mainly due to the difficulty in obtaining well-defined
confined spaces allowing a simultaneous study of the mineralization
process outside and inside the cavities. Herein, we have studied magnetite
precipitation in the channels of cross-linked protein crystals (CLPCs)
with different channel pore sizes, as a model of crystallization in
confined spaces. Our results show that nucleation of an Fe-rich phase
occurs inside the protein channels in all cases, but, by a combination
of chemical and physical effects, the channel diameter of CLPCs exerted
a precise control on the size and stability of those Fe-rich nanoparticles.
The small diameters of protein channels restrain the growth of metastable
intermediates to around 2 nm and stabilize them over time. At larger
pore diameters, recrystallization of the Fe-rich precursors into more
stable phases was observed. This study highlights the impact that
crystallization in confined spaces can have on the physicochemical
properties of the resulting crystals and shows that CLPCs can be interesting
substrates to study this process
In situ real-time monitoring of the mechanism of self-assembly of short peptide supramolecular polymers
Making use of the combination of multiparametric Fluorescence Lifetime Imaging Microscopy (FLIM) and single-molecule Fluorescence Lifetime Correlation Spectroscopy (FLCS), we have been able to study for the early stages of the fluorenylmethyloxycarbonyl-diphenylalanine (Fmoc-FF) self-assembly process with single-molecule resolution, the kinetics of fiber formation, the packaging of the peptides within the fibers and the capacity of the peptides to reassemble after disruption (self-healing) in the presence of different metallic cations. Other techniques such as FTIR, TEM, DSC and DFT calculations support our findings. The impact that the mechanism of self-assembly has on the physical (rigidity and self-healing) properties of the resulting gels have also been evaluated by rheology. Calcium ions are able to promote the self-assembly of Fmoc-FF faster and more efficiently, forming more rigid hydrogels than do cesium ions. The reasons behind this effect may be explained by the different capacities that these two cations have to coordinate with the peptide, modulate its hydrophobicity and stabilize the water-solute interphase. These findings shed light on the impact that small changes have on the process of self-assembly and can help to understand the influence of the environmental conditions on the in vivo uncontrolled self-assembly of certain proteins