Postmortem biochemistry

Postmortem biochemistry is becoming more important in forensic pathology. Involving of biochemical investigations full autopsy can provide to detect divers pathologic conditions such as antemortem acute/chronic diseases, fatal metabolic conditions, survival time, postmortem biochemical changes, and the source of analytes. Biochemical tests may be usefull where the morphological examinations cannot lighten the fatal pathology (Diabetes Mellitus (DM), alcoholic ketoacidosis, sepsis, electrolytic disorders). This article presents the analytes that may be useful in forensic medicine upon the studies performed and published in the literature

Severe methanol intoxication due to spirit consumption and magnetic resonance imaging findings İspirto tüketimine baǧli{dotless} ciddi metanol zehirlenmesi ve manyetik rezonans görüntü bulgulari{dotless}

Acute methanol intoxication may result in a wide range of damage to the central nervous system. Most patients are typically admitted to the hospital for several days after methanol ingestion, presenting with visual disruptions, severe acidosis, or both. Rapid diagnosis and treatment are necessary to prevent death and to minimize the neurological sequelae. We want to emphasize the importance of specific MRI findings in a patient, who had drunk a bottle of beer and 70 mL of spirits, and was admitted to hospital after 20 hours, with severe metabolic acidosis and loss of consciousness. A methanol assay was unavailable. © 2013 by Erciyes University School of Medicine

Interleukin-1 Induced Nuclear Factor-B Binds to a Disintegrin-Like and Metalloproteinase with Thrombospondin Type 1 Motif 9 Promoter in Human Chondrosarcoma Cells

Nuclear factor-B (NF-B) is involved in the regulation of inflammation-associated genes. NF-B forms dimers which bind with sequences referred to as NF-B sites (9-11 bp). A disintegrin-like and metalloproteinase with thrombospondin type 1 motif 9 (ADAMTS9) is a type of proteoglycanase, which proteolytically cleaves versican and aggrecan. ADAMTS9 is a cytokine-inducible gene that contains binding sites for NF-B within its promoter region. Interleukin-1 (IL-1) affects cartilage metabolism and is involved in the NF-B pathway. It is therefore hypothesized that NF-B binding with ADAMTS9 promoters may activate IL-1, thereby promoting chondrocytic cell growth. In the present study, the OUMS-27 chondrocytic human chondrosarcoma cell line was treated with IL-1 with or without inhibitors of NF-B signaling pathways. Chromatin immunoprecipitation (ChIP) and electromobility shift assays (EMSA) were conducted order to analyze the binding of NF-B with the ADAMTS9 promoter region. NF-B-p65 subunit phosphorylation was promoted in IL-1-treated cells, which were not treated with inhibitors of NF-B signaling pathways. By contrast, NF-B-p65 subunit phosphorylation was inhibited in cells that had been treated with BAY-117085, an NF-B pathway inhibitor. ChIP and EMSA assays demonstrated that, following treatment with IL-1, NF-B-p65 bound to elements located at -1177 and -1335 in the ADAMTS9 promoter region, in contrast to the untreated samples. The results of the present study suggested that NF-B may be involved in IL-1-induced activation of ADAMTS9 in human chondrocytes.WoSScopu