Object-based modelling for representing and processing speech corpora

This thesis deals with modelling data existing in large speech corpora using an object-oriented paradigm which captures important linguistic structures. Information from corpora is transformed into objects and are assigned properties regarding their behaviour. These objects, called speech units, are placed onto a multi-dimensional framework and have their relationships to other units explicitly defined through the use of links. Frameworks that model temporal utterances or atemporal information like speaker characteristics and recording conditions can be searched efficiently for contextual matches. Speech units that match desired contexts are the result of successful linguistically motivated queries and can be used in further speech processing tasks in the same computational environment. This allows for empirical studies of speech and its relation to linguistic structures to be carried out, and for the training and testing of applications like speech recognition and synthesis. Information residing in typical speech corpora is discussed first, followed by an overview of object-orientation which sets the tone for this thesis. Then the representation framework is introduced which is generated by a compiler and linker that rely on a set of domain-specific resources that transform corpus data into speech units. Operations on this framework are then presented along with a comparison between a relational and object-oriented model of identical speech data. The models described in this work are directly applicable to existing large speech corpora, and the methods developed here are tested against relational database methods. The object-oriented methods outperform the relational methods for typical linguistically relevant queries by about three orders of magnitude as measured by database search times. This improvement in simplicity of representation and search speed is crucial for the utilisation of large multi-lingual corpora in basic research on the detailed properties of speech, especially in relation to contextual variation.reviewe

Youth of West Cameroon are at high risk of developing IDD due to low dietary iodine and high dietary thiocyanate.

Objectives: Hypothyroidism in utero leading to mental retardation is highly prevalent and recurrent in developing countries where iodine deficiency and thiocyanate overload are combined. So, to explore and identify human population's risks for developing iodine deficiency disorders and their endemicity in Western Cameroon, with the aim to prevent this deficiency and to fight again it, urinary iodine and thiocyanate levels were determined. Methods: The district of Bamougoum in Western Cameroon was selected for closer study due to its geographic location predisposing for iodine deficiency disorders (IDD). A comprehensive sampling strategy included 24-h urine samples collected over three days from 120 school-aged children. Urinary iodine and thiocyanate levels were measured by colorimetric methods. Results: Twenty one percent of boys between the ages 3 and 19 were classified as iodine deficient. The prevalence of thiocyanate overload in the same population was found to be 20%. Conclusion: Presence of endemic iodine deficiency and excessive thiocyanate in the population indicates that the region is at risk of iodine deficiency disorder. A multifactorial approach that includes improvement of diet, increasing iodine and minimizing goitrogen substances intake, soil and crop improvement and an iodine supplementation program may help alleviate IDD in the affected area studied. African Health Sciences Vol. 8 (4) 2008: pp. 227-23

Identification of three wheat globulin genes by screening a Triticum aestivum BAC genomic library with cDNA from a diabetes-associated globulin

<p>Abstract</p> <p>Background</p> <p>Exposure to dietary wheat proteins in genetically susceptible individuals has been associated with increased risk for the development of Type 1 diabetes (T1D). Recently, a wheat protein encoded by cDNA WP5212 has been shown to be antigenic in mice, rats and humans with autoimmune T1D. To investigate the genomic origin of the identified wheat protein cDNA, a hexaploid wheat genomic library from Glenlea cultivar was screened.</p> <p>Results</p> <p>Three unique wheat globulin genes, <it>Glo-3A</it>, <it>Glo3-B </it>and <it>Glo-3C</it>, were identified. We describe the genomic structure of these genes and their expression pattern in wheat seeds. The <it>Glo-3A </it>gene shared 99% identity with the cDNA of WP5212 at the nucleotide and deduced amino acid level, indicating that we have identified the gene(s) encoding wheat protein WP5212. Southern analysis revealed the presence of multiple copies of <it>Glo-3</it>-like sequences in all wheat samples, including hexaploid, tetraploid and diploid species wheat seed. Aleurone and embryo tissue specificity of WP5212 gene expression, suggested by promoter region analysis, which demonstrated an absence of endosperm specific <it>cis </it>elements, was confirmed by immunofluorescence microscopy using anti-WP5212 antibodies.</p> <p>Conclusion</p> <p>Taken together, the results indicate that a diverse group of globulins exists in wheat, some of which could be associated with the pathogenesis of T1D in some susceptible individuals. These data expand our knowledge of specific wheat globulins and will enable further elucidation of their role in wheat biology and human health.</p

Preliminary Evaluation of Lectins as Fluorescent Probes of Seed Structure and Composition

Several commercially available fluoresceinisothiocyanate and rhodamine isothiocyanateconjugated plant lectins have been applied to cereal and oilseed tissues to permit identification and localization of specific structures and carbohydrates by fluorescence microscopy . Ulex europeaus Agglutinin I (UEAl) and Ricinis communis Agglutinin I (RCA I) showed specificity for the amyloids in rapeseed cotyledonary cell walls . Wheat Germ Agglutinin (WGA) bound to rapeseed coat mucilage, as well as fungal hyphae in infected wheat . Lens culinaris Agglutinin (LCA) bound only to starch in cereal sections, and at higher magnifications of isolated starch granules , the annular structure was clearly visible