Разработка информационной системы для автоматизации учета шурфовочных работ на магистральном газопроводе

Разработанная информационная система "Мониторинг шурфовочных работ" необходима для мониторинга и ввода данных по выполняемым работам, связанным с подтверждением и устранением дефектов на магистральном газопроводе, на объектах в зоне ответственности ООО "Газпром трансгаз Томск".The developed information system "Monitoring of excavations" is necessary for monitoring and input of data on the work performed related to the confirmation and elimination of defects on the main gas pipeline at the facilities in the zone of responsibility of Gazprom transgaz Tomsk

PARP1 ADP-ribosylates lysine residues of the core histone tails

The chromatin-associated enzyme PARP1 has previously been suggested to ADP-ribosylate histones, but the specific ADP-ribose acceptor sites have remained enigmatic. Here, we show that PARP1 covalently ADP-ribosylates the amino-terminal histone tails of all core histones. Using biochemical tools and novel electron transfer dissociation mass spectrometric protocols, we identify for the first time K13 of H2A, K30 of H2B, K27 and K37 of H3, as well as K16 of H4 as ADP-ribose acceptor sites. Multiple explicit water molecular dynamics simulations of the H4 tail peptide into the catalytic cleft of PARP1 indicate that two stable intermolecular salt bridges hold the peptide in an orientation that allows K16 ADP-ribosylation. Consistent with a functional cross-talk between ADP-ribosylation and other histone tail modifications, acetylation of H4K16 inhibits ADP-ribosylation by PARP1. Taken together, our computational and experimental results provide strong evidence that PARP1 modifies important regulatory lysines of the core histone tail

Электропривод установки вибромагнитной обработки донных отложений нефти

Цель работы? провести расчет и исследование электропривода резонансного вибратора модульного типа, предназначенного для предотвращения и для удаления донных отложений нефти в резервуарах. В работе разработана математическая модель, которая позволяет исследовать режимы работы вибратора, произведен выбор преобразователя частоты и других элементов электропривода. С помощью математической модели, решаемой в программе Mathcad 14, проведено исследование режимов работы установки, получены основные параметры, необходимые для выбора силового оборудования электропривода.The aim of this work is to calculate and study of the electric resonant vibrator of modular type, intended for the prevention and removal of bottom sediments of oil in the tanks. In this paper we developed a mathematical model that allows to explore the modes of operation of the vibrator, selection of frequency Converter and other elements of the drive. Using a mathematical model solved in Mathcad 14, the study of the modes of the unit received the basic parameters required for selection of power equipment electric

PARP1 ADP-ribosylates lysine residues of the core histone tails

The chromatin-associated enzyme PARP1 has previously been suggested to ADP-ribosylate histones, but the specific ADP-ribose acceptor sites have remained enigmatic. Here, we show that PARP1 covalently ADP-ribosylates the amino-terminal histone tails of all core histones. Using biochemical tools and novel electron transfer dissociation mass spectrometric protocols, we identify for the first time K13 of H2A, K30 of H2B, K27 and K37 of H3, as well as K16 of H4 as ADP-ribose acceptor sites. Multiple explicit water molecular dynamics simulations of the H4 tail peptide into the catalytic cleft of PARP1 indicate that two stable intermolecular salt bridges hold the peptide in an orientation that allows K16 ADP-ribosylation. Consistent with a functional cross-talk between ADP-ribosylation and other histone tail modifications, acetylation of H4K16 inhibits ADP-ribosylation by PARP1. Taken together, our computational and experimental results provide strong evidence that PARP1 modifies important regulatory lysines of the core histone tails