Evaluation of the Pesticide Emamectin and Methanol Extract of Wheat Bran against Biomphalaria Alexandrina Snails, Their Hemocytes and Their Infection with Schistosoma Mansoni

The present study was carried out to evaluate the molluscicidal activity of the pesticide Emamectin (5% aqueous solution) and the methanol extract from the wheat bran (MEWB) against B alexandrina and their infection with Schistosoma mansoni was studied. The LC90 and LC50 values for Emamectin were 50.4 and 22.3 ppm, respectively. Infection of snails with S. mansoni under the effect of the tested agents was evaluated via four experimental groups, each of 50 snails For three consecutive days, one group of snails was exposed to 9.08 ppm aqueous Emamectin solution, another group received 100 ppm methanol extract of wheat bran (MEWB), a third group was administered by a combination of 9.08 ppm Emamectin and 100ppm MEWB, The fourth group was control maintained under similar experimental conditions. After three days, all the experimental groups were infected with S. mansonimiracidia and observed till shedding of cercariae. The physiological and histological changes were assessed before and after the infection.The changes in hemocytes of infected snails after administration with LC25 of Emamectin or wheat bran was significantly (20% and 30%, respectively) suppressed compared to 75% for the control snails. However, the snails treated with joint of Emamectin and MEWB were the least infected snails (10%). On the other hand, the biochemical test results showed a remarkable reduction of GOT (p<0.01) GPT (p<0.05) and total protein (0.05) in the heamolyph extracted from the snails treated with an aqueous Emamectin solution. Yet, the levels of GOT were significantly increased in the groups administered with the MEWB alone (p<0.01) or in combination with the Emamectin (p<0.001). As for the total protein levels, there were slightly declining (p<0.05) in the group exposed to the aqueous Emamectin and to the contrary, theses levels were significantly increased (P<0.05) in the snails of the two other experimental groups when compared to the controls. Moreover, the haemocytes cells showed a differentiation which varied in number when detected under the microscope. Administration of the aqueous Emamectin solution resulted in a significant increase (p<0.05) of the amaebocytes and a considerable decrease (p<0.05) in the number of granulocytes and hyalinocytes compared to the control snails. On the contrary, a remarkable surge (P<0.05) of granulocytes and decrease of the amaebocytes was detected in the haemolyph of snails treated with MEWB

IMMUNOHISTOCHEMICAL STUDY OF THE INFLAMMATORY INFILTRATE IN VILLITIS OF UNKNOWN ETIOLOGY - A QUALITATIVE AND QUANTITATIVE-ANALYSIS

Villitis is characterized by an inflammatory infiltrate within the substance of the chorionic villi. Quantitative and qualitative analyses of the mononuclear infiltrate in areas of villitis were performed in placentas with villitis of unknown etiology (VUE). We used a panel of monoclonal antibodies and immunoperoxidase technique in paraffin sections from 17 placentas with VUE and 8 without VUE. Macrophages followed by T lymphocytes were the predominant inflammatory cells in areas of villitis in virtually all cases. B lymphocytes were not observed and monocytes were present usually in small number in 58 per cent of the cases. Mononuclear cells which expressed HLA-DR antigens were found in 75 per cent of the cases. In areas of villitis with trophoblastic necrosis, we found monocytes and some T lymphocytes adhered to them. These cells apparently had migrated from the maternal circulation. We suggest that in areas of villitis with destruction of the trophoblast and its basal membrane the inflammatory infiltrate might have a mixture of fetal and maternal cells. The maternal monocytes and T lymphocytes might be attracted to these sites of trophoblastic necrosis and activated due to exposure to fetal MHC antigens of the villous stroma.188330330

THROMBOSIS OF FETAL PLACENTAL VESSELS - A QUANTITATIVE STUDY IN PLACENTAS OF STILLBIRTHS

182568568

NON-THROMBOTIC CHANGES OF FETAL PLACENTAL VESSELS IN STILLBIRTHS - A QUALITATIVE AND QUANTITATIVE STUDY

182567668

Eosinophilic angiocentric fibrosis of the nasal cavity

Eosinophilic angiocentric fibrosis (EAF) is a rare condition of unknown etiology that causes stenosis of the upper respiratory tract, We report one case in which immunohistochemical studies were performed that involved the mucosa of the lateral nasal wall and septum of a 54-year-old woman, The lesion showed areas with vascular proliferation, small-vessel vasculitis, and dense inflammatory infiltrate consisting of T lymphocytes, macrophages, polyclonal plasma cells, and numerous eosinophils and neutrophils, Other areas were hypocellular and fibrotic, and in those, the collagen bundles showed perivascular onion-skin whorling, The clinical and histologic similarities between our case and those previously described, in addition to the immunohistochemical findings, support the hypothesis that eosinophilic angiocentric fibrosis is a chronic inflammatory condition with a peculiar and striking stromal response.10439139

HEMORRHAGIC ENDOVASCULITIS OF THE PLACENTA - A CLINICAL-PATHOLOGICAL STUDY IN BRAZIL

Hemorrhagic endovasculitis (HEV) of the placenta involves damage to or destruction of chorionic vessels and fetal erythrocytes. We evaluated the frequency and extension of the HEV and clinical events and morphologic changes associated with it in placentas from live-born and stillborn infants. Two hundred sixty four placentas were examined: 214 from livebirths and 50 from stillbirths. The livebirths were subdivided according to the time of intrauterine retention (IUR). HEV occurred in 8.8% of placentas from livebirths and in 32% from stillbirths. In the livebirths the lesion affected small number of villi, was frequently associated with villitis (73.6% of the cases), but there was no significant association with maternal disease or birth weight. In the stillbirths, HEV was strongly associated with the time of IUR. In stillbirths with 1 day of IUR, HEV occurred in frequency similar to the livebirths (9.5%), but in stillbirths with 2-7 days of IUR the frequency was high (71.4%) and numerous vessels were involved. In those over 7 days the lesion was less extensive and frequent (20%) and it had the appearance of end stage of organization. We conclude that HEV, in livebirths and fresh stillbirths is frequently a small lesion of little significance, while in macerated stillbirths it is an artifact of maceration.o TEXTO COMPLETO DESTE ARTIGO, ESTARÁ DISPONÍVEL À PARTIR DE AGOSTO DE 2015.23535936

CORD IGM LEVELS IN PLACENTAS WITH VILLITIS OF UNKNOWN ETIOLOGY

o TEXTO COMPLETO DESTE ARTIGO, ESTARÁ DISPONÍVEL À PARTIR DE AGOSTO DE 2015.17646546

CONGENITAL FOCAL GLOMERULAR-LESIONS IN ONLY ONE MONOZYGOTIC TWIN RELATED TO A PROBABLE TWIN TRANSFUSION SYNDROME

10999199

Immunohistochemical characterization of the inflammatory infiltrate in placental Chagas' disease: A qualitative and quantitative analysis

Chagas' disease, a systemic illness endemic to some regions of South America, is caused by the protozoan Trypanosoma cruzi. Transplacental infection may occur during any phase and cause fetal death. This study is the first to characterize the inflammatory cells in chagasic villitis by immunohistochemistry. Paraffin sections of 8 placentas with villitis by T. cruzi (4 live births and 4 stillbirths), as well as 8 control placentas without inflammation, were stained with hematoxylin and eosin, monoclonal antibodies for CD45RO, CD20, CD45RO/OPD4, CD8, HNK1, CD15, MAC387, and CD68 proteins, and a polyclonal antibody for S-100 protein. Quantification of positive cells was performed in 3 different high-power fields. In all cases of chagasic villitis, the inflammatory infiltrate was composed mainly of CD68+ macrophages, T lymphocytes, and a few natural killer cells. Among T cells, CD8+ cells outnumbered CD4+ cells in all placentas (CD4+:CD8+ ratios ranged from 0.04 to 0.38). B cells were absent or rare. In stillbirths, villitis was diffuse and severe with numerous T. cruzi, while in live births it was focal with few parasites. Other features that characterized villitis in stillbirths were 1) frequent trophoblastic necrosis, 2) presence of MAC387+ macrophages and CD15+ granulocytes attached to the sites of trophoblastic necrosis, 3) low CD4+: CD8+ ratios in most cases, 4) increased numbers of S-100 positive cells in the villous stroma. In conclusion, CD68+ macrophages and CD8+ T lymphocytes were the major cell population in villitis caused by T. cruzi. However, the pattern of inflammatory reaction differed between stillbirths and live births and was probably related to the number of parasites in the placental villi.o TEXTO COMPLETO DESTE ARTIGO, ESTARÁ DISPONÍVEL À PARTIR DE AGOSTO DE 2015.62231932