Analysis of corticosteroids in samples of animal origin using QuEChERS and ultrahigh-performance liquid chromatography coupled to high-resolution mass spectrometry.

A rapid and sensitive method for the confirmatory analysis of 8 synthetic corticosteroids (betamethasone, dexamethasone, prednisolone, 6 methyl prednisolone, triamcinolone, flumethasone, beclomethasone, fluocinolone acetonide) is proposed. The method is useful for detecting illegal treatments in different animal species. It consists of an extraction and clean-up using the Quick Easy Cheap Effective Rugged Safe (QuEChERS) strategy. Quantitative determination is achieved by ultra-high performance liquid chromatography coupled to high-resolution mass spectrometry with heated electrospray ionization in negative mode. Quantification is performed using surrogate matrix matched standard calibration curve with dexamethasone-D4 as the internal standard. The method was validated for analyzing liver samples according to the criteria established by Decision 2002/657/EC. Linearity was assessed in the 1-10 μg kg-1 range and linear correlation coefficients were over 0.99 for all the analytes. CCα ranged from 0.04 to 0.16 μg kg-1 for substances without maximum residue limit. The method allows confident quantification and confirmation of corticosteroids in liver samples, and its simplicity makes it suitable for analyzing large numbers of samples

Black Holes in Pseudobulges and Spheroidals: A Change in the Black Hole-Bulge Scaling Relations at Low Mass

We investigate the relationship between black hole mass and host galaxy properties for active galaxies with the lowest black hole masses currently known in galaxy nuclei. Hubble Space Telescope imaging confirms that the host galaxies have correspondingly low luminosity; they are ~1 mag below L*. In terms of morphology, ~60% of the members of the sample are disk-dominated, and all of these are consistent with containing a bulge or (more likely) pseudobulge, while the remainder are compact systems with no discernible disk component. In general the compact components of the galaxies do not obey the fundamental plane of giant elliptical galaxies and classical bulges, but rather are less centrally concentrated at a given luminosity, much like spheroidal galaxies. Our results strongly confirm that a classical bulge is not a requirement for a nuclear black hole. At the same time, the observed ratio of black hole to bulge mass is nearly an order of magnitude lower in this sample than that seen for classical bulges. While the M-sigma relation appears to continue to low mass, it seems that black hole-galaxy scaling relations do depend on galaxy structure.Comment: to appear in ApJ; 22 pages; 8 figures; original version available at http://www.astro.princeton.edu/~jgreene/paper.pd

Behavioral and clinical characteristics of people receiving medical care for HIV infection in an outpatient facility in Sicily, Italy

Aim: The authors examined a cohort of HIV-positive outpatients at the AIDS Center of Palermo University in Italy in order to identify factors related to the frequency of their visits to the outpatient facility for health care services. Methods: Two hundred and twenty-four HIV-infected subjects were enrolled in the study. Demographic and HIV disease characteristics were recorded and assessed with the number of days accessed to our outpatients unit in univariate and multivariate analyses. The potential relationship with immunological status was also analyzed stratifying the patients into groups according to their CD4+ T-cell counts ( 65500 vs, \u2c2500/mm3, and 65200 vs < 200/mm3). Results: Both univariate and multivariate analyses showed that duration of antiretroviral therapy, \u2c25 years and hypertension were significantly associated with a CD4+ T-cell count of, \u2c2500/mm3, whereas geographic origin (Africa) was associated with a CD4+ T-cell count of, \u2c2200/mm3. Mean number of days the patients sought access to day-care services for laboratory tests was negatively associated with CD4+ T-cell count. Conclusion: Patients with low CD4+ T-cell counts showed higher use of health care services, demonstrating how early HIV diagnosis can help to reduce health care costs. The CD4+ T-cell cut-off of 200 cells emphasizes the importance of identifying and managing HIV infection among hard-to-reach groups like vulnerable migrants. In our sle, the illegal status of immigrants does not influence the management of their HIV/AIDS condition, but the lack of European health card that documents the current antiretroviral status, could interfere with the efforts to eradicate AIDS. A better understanding of the major determinants of HIV treatment costs has led to appropriate large-scale actions, which in turn has increased resources and expanded intervention programs. Further guidance should be offered to hard-to-reach groups in order to improve early AIDS diagnosis, and procedures for identifying and managing these vulnerable subjects should be made available to care commissioners and service providers

Intermediate mass stars: updated models

A new set of stellar models in the mass range 1.2 to 9 $M_{\odot}$ is presented. The adopted chemical compositions cover the typical galactic values, namely $0.0001 \le Z \le 0.02$ and $0.23 \le Y \le 0.28$. A comparison among the most recent compilations of similar stellar models is also discussed. The main conclusion is that the differencies among the various evolutionary results are still rather large. For example, we found that the H-burning evolutionary time may differ up to 20 %. An even larger disagreement is found for the He-burning phase (up to 40-50 %). Since the connection between the various input physics and the numerical algorithms could amplify or counterbalance the effect of a single ingredient on the resulting stellar model, the origin of this discrepancies is not evident. However most of these discrepancies, which are clearly found in the evolutionary tracks, are reduced on the isochrones. By means of our updated models we show that the ages inferred by the theory of stellar evolution is in excellent agreement with those obtained by using other independent methods applied to the nearby Open Clusters. Finally, the theoretical initial/final mass relation is revised.Comment: 35 pages, 24 figures, 4 tables, accepted for publication in the Astrophisycal Journa

A Spectroscopic Study of the Ancient Milky Way: F- and G-Type Stars in the Third Data Release of the Sloan Digital Sky Survey

(Abridged) We perform an analysis of spectra and photometry for 22,770 stars included in the third data release (DR3) of the SDSS. We measure radial velocities and, based on a model-atmosphere analysis, derive estimates ofthe atmospheric parameters (effective temperature, surface gravity, and [Fe/H]) for each star. Stellar evolution models are then used to estimate distances. The SDSS sample covers a range in stellar brightness of 14 < V < 22, and comprises large numbers of F- and G-type stars from the thick-disk and halo populations (up to 100 kpc from the galactic plane), therefore including some of the oldest stars in the Milky Way. In agreement with previous results from the literature, we find that halo stars exhibit a broad range of iron abundances, with a peak at [Fe/H] ~ -1.4. This population exhibits essentially no galactic rotation. Thick-disk G-dwarf stars at distances from the galactic plane in the range 1<|z|<3 kpc show a much more compact metallicity distribution, with a maximum at [Fe/H] ~ -0.7, and a median galactic rotation velocity at that metallicity of 157 +/- 4 km/s (a lag relative to the thin disk of 63 km/s). A comparison of color indices and metal abundances with isochrones indicates that no significant star formation has taken place in the halo in the last ~ 11 Gyr, but there are thick-disk stars which are at least 2 Gyr younger. We find the metallicities of thick-disk stars to be nearly independent of galactocentric distance between 5 and 14 kpc, in contrast with the marked gradients found in the literature for the thin disk. No vertical metallicity gradient is apparent for the thick disk, but we detect a gradient inits rotational velocity of -16 +/- 4 km/s/kpc between 1 and 3 kpc from the plane.Comment: 18 pages, 16 figures; accepted for publication in the ApJ; also available from http://hebe.as.utexas.edu

The s-Process in Rotating Asymptotic Giant Branch Stars

(abridged) We model the nucleosynthesis during the thermal pulse phase of a rotating, solar metallicity AGB star of 3M_sun. Rotationally induced mixing during the thermal pulses produces a layer (~2E-5M_sun) on top of the CO-core where large amounts of protons and C12 co-exist. We follow the abundance evolution in this layer, in particular that of the neutron source C13 and of the neutron poison N14. In our AGB model mixing persists during the entire interpulse phase due to the steep angular velocity gradient at the core-envelope interface. We follow the neutron production during the interpulse phase, and find a resulting maximum neutron exposure of tau_max =0.04 mbarn^-1, which is too small to produce any significant s-process. In parametric models, we then investigate the combined effects of diffusive overshooting from the convective envelope and rotationally induced mixing. Models with overshoot and weaker interpulse mixing - as perhaps expected from more slowly rotating stars - yield larger neutron exposures. We conclude that the incorporation of rotationally induce mixing processes has important consequences for the production of heavy elements in AGB stars. Through a distribution of initial rotation rates it may lead to a natural spread in the neutron exposures obtained in AGB stars of a given mass - as appears to be required by observations. Our results suggest that both processes, diffusive overshoot and rotational mixing, may be required to obtain a consistent description of the s-process in AGB stars which fulfils all observational constraints. Finally, we find that mixing due to rotation within our current framework does increase the production of N15 in the partial mixing zone, however still falling short of what seems required by observations.Comment: 50 pages, 13 figures, ApJ in press, tentatively scheduled for v593 n2 August 20, 200

Leptomeningeal disease and brain control after postoperative stereotactic radiosurgery with or without immunotherapy for resected brain metastases

Purpose Immunotherapy has shown activity in patients with brain metastases (BM) and leptomeningeal disease (LMD). We have evaluated LMD and intraparenchymal control rates for patients with resected BM receiving postoperative stereotactic radiosurgery (SRS) and immunotherapy or postoperative SRS alone. We hypothesize that postoperative SRS and immunotherapy will result in a lower rate of LMD with acceptable toxicity compared with postoperative SRS. Patients and methods One hundred and twenty-nine patients with non-small-cell lung cancer (NSCLC) and melanoma BM who received postoperative fractionated SRS (fSRS; 3×9 Gy) in combination with immunotherapy or postoperative fSRS alone for completely resected BM were retrospectively evaluated. The primary endpoint of the study was the rate of LMD after treatments. The secondary endpoints were local failure, distant brain parenchymal failure (DBF), overall survival (OS), and treatment-related toxicity. Results Sixty-three patients received postoperative SRS and immunotherapy, either nivolumab or pembrolizumab, and 66 patients received postoperative SRS alone to the resection cavity. With a median follow-up of 15 months, LMD occurred in 19 patients: fSRS group, 14; fSRS and immunotherapy, 5. The 12-month LMD cumulative rates were 22% (95% CI 14% to 37%) in the fSRS group and 6% (95% CI 2% to 17%) in the combined treatment group (p=0.007). Resection cavity control was similar between the groups, whereas DBF and OS were significantly different; the 1-year DBF rates were 31% (95% CI 20% to 46%) in the fSRS and immunotherapy group and 52% (95% CI 39% to 68%) in the fSRS group; respective OS rates were 78% (95% CI 67% to 88%) and 58.7% (95% CI 47% to 70%). Twenty-two patients undergoing postoperative fSRS and immunotherapy and nine subjected to postoperative fSRS experienced treatment-related imaging changes suggestive of radiation-induced brain necrosis (p=0.02). Conclusions Postoperative fSRS in combination with immunotherapy decreases the incidence of LMD and DBF in patients with resected BM from NSCLC and melanoma as compared with fSRS alone, reducing the rate of neurological death and prolonging survival

On the Delta V_HB_bump parameter in Globular Clusters

We present new empirical estimates of the Delta V_HB_bump parameter for 15 Galactic globular clusters (GGCs) using accurate and homogeneous ground-based optical data. Together with similar evaluations available in the literature, we ended up with a sample of 62 GGCs covering a very broad range in metal content (-2.16<=[M/H]<=-0.58 dex). Adopting the homogeneous metallicity scale provided either by Kraft & Ivans (2004) or by Carretta et al. (2009), we found that the observed Delta V_HB_bump parameters are larger than predicted. In the metal-poor regime ([M/H]<=-1.7, -1.6 dex) 40% of GCs show discrepancies of 2sigma (~0.40 mag) or more. Evolutionary models that account either for alpha- and CNO-enhancement or for helium enhancement do not alleviate the discrepancy between theory and observations. The outcome is the same if we use the new Solar heavy-element mixture. The comparison between alpha- and CNO-enhanced evolutionary models and observations in the Carretta et al. metallicity scale also indicates that observed Delta V_HB_bump parameters, in the metal-rich regime ([M/H]=>0), might be systematically smaller than predicted

The giant, horizontal and asymptotic branches of galactic globular clusters. I. The catalog, photometric observables and features

A catalog including a set of the most recent Color Magnitude Diagrams (CMDs) is presented for a sample of 61 Galactic Globular Clusters (GGCs). We used this data-base to perform an homogeneous systematic analysis of the evolved sequences (namely, Red Giant Branch (RGB), Horizontal Branch (HB) and Asymptotic Giant Branch (AGB)). Based on this analysis, we present: (1) a new procedure to measure the level of the ZAHB (V_ZAHB) and an homogeneous set of distance moduli obtained adopting the HB as standard candle; (2) an independent estimate for RGB metallicity indicators and new calibrations of these parameters in terms of both spectroscopic ([Fe/H]_CG97) and global metallicity ([M/H], including also the alpha-elements enhancement). The set of equations presented can be used to simultaneously derive a photometric estimate of the metal abundance and the reddening from the morphology and the location of the RGB in the (V,B-V)-CMD. (3) the location of the RGB-Bump (in 47 GGCs) and the AGB-Bump (in 9 GGCs). The dependence of these features on the metallicity is discussed. We find that by using the latest theoretical models and the new metallicity scales the earlier discrepancy between theory and observations (~0.4 mag) completely disappears.Comment: 51 pages, 23 figures, AAS Latex, macro rtrpp4.sty included, accepted by A

Natural and anthropogenic changes to mangrove distributions in the Pioneer River Estuary (QLD, Australia)

We analyzed a time series of aerial photographs and Landsat satellite imagery of the Pioneer River Estuary (near Mackay, Queensland, Australia) to document both natural and anthropogenic changes in the area of mangroves available to filter river runoff between 1948 and 2002. Over 54 years, there was a net loss of 137 ha (22%) of tidal mangroves during four successive periods that were characterized by different driving mechanisms: (1) little net change (1948– 1962); (2) net gain from rapid mangrove expansion (1962–1972); (3) net loss from clearing and tidal isolation (1972–1991); and (4) net loss from a severe species-specific dieback affecting over 50% of remaining mangrove cover (1991–2002). Manual digitization of aerial photographs was accurate for mapping changes in the boundaries of mangrove distributions, but this technique underestimated the total loss due to dieback. Regions of mangrove dieback were identified and mapped more accurately and efficiently after applying the Normalized Difference Vegetation Index (NDVI) to Landsat Thematic Mapper satellite imagery, and then monitoring changes to the index over time. These remote sensing techniques to map and monitor mangrove changes are important for identifying habitat degradation, both spatially and temporally, in order to prioritize restoration for management of estuarine and adjacent marine ecosystems