The mapping count table

This count table was used as an input for differential gene expression analysis in edge

GO term reduction table

Gene Ontology terms were summarized and redundant GO terms were removed by semantic similarity-based approach employed in REViGO

Sequences of S. lalandi transcriptome (fasta file)

Transcriptome contigs assembled from filtered paired end sequencing reads on Illumina HiSeq™ 2000 platform using Trinity. Only non-redundant (cluster in CAP3), more than 300bp contigs with open reading frame were kep

Table containing differential expressed genes (result of edgeR)

This table contains data of list of genes that were < 0.01 FDR value and differed in their expression value at least 2 fold between two group

Information on SNPs identified from transcriptome data (vcf file)

The details of SNPs calling are described in Materials and method

Design, synthesis, molecular docking, and biological studies of novel phytoestrogen-tanaproget hybrids

<p>A diverse range of novel and highly functionalized flavonoid-based tanaproget hybrids were synthesized and evaluated in vitro for their antimicrobial and antiproliferative activities. Novel products were synthesized in good yields (81–95%) under Pd-catalyzed reaction from bromo flavones and tanaproget boronic acids within 18–20 min at 60 °C. Bioassay results exhibited excellent activities against both hormone-dependent and hormone-independent human breast cancer cells (MCF-7, MDA-MB-231, DU-145, PC-3, and HeLa). Among them, compounds <b>4e, 9a, 9c, 9e, 9 g, 9 h, 9 m,</b> and <b>9n</b> displayed excellent activity. Compounds <b>4d, 4o,</b> and <b>9o</b> were found equally potent against <i>C. albicans</i> compared to fluconazole. Compound <b>5c</b> showed better antibacterial activity against <i>S. aureus</i>. Compounds <b>5a, 9i, 9o,</b> and <b>10c</b> have shown admirable antibacterial activity against <i>E. coli</i>.</p

Insights into the Enhanced Lipid Production Characteristics of a Fresh Water Microalga under High Salinity Conditions

Bioprospecting of microalgae capable of growing and accumulating high amounts of lipids in high salinity conditions such as seawater can substantially improve the economic vaibaility of algal biodiesel production. In view of this, a fresh water microalga, <i>Scenedesmus</i> sp. IITRIND2, was cultivated under saline conditions to assess its halotolerant behavior and potential as biodiesel feedstock. The microalga efficiently adapted to 100% seawater salinity, enhanced its lipid content by 52%, thus yielded ∼3.2 fold higher lipid productivity as compared to the Bold’s basal media (BBM). The increase in the lipid content was balanced by a sharp decrease in its protein and carbohydrate content. Biochemical analysis evidenced that salinity induced oxidative stress resulted in reduced levels of photosynthetic pigments, elevated levels of reactive oxygen species (H₂O₂, thiobarbituric acid reactive substances), osmolytes (proline, glycine betaine), and activity of antioxidant enzymes (catalase, ascorbate peroxidase). These studies suggested that microalga efficiently modulated its metabolic flexibility in order to acclatamize the salanity induced stress. Further, the FAME analysis revealed the dominance of C14:0, C16:0, C18:0, C18:1, and C18:2 fatty acids under halotolerant conditions, and the properties of the resulting biodiesel were in compliance with ASTM (American Society for Testing Materials) D6751 and EN 14214 (European) fuel standards. These results consolidate that the lipid augmented halotolerant algal strains capable of growing in saline/seawater can be formulated as environmental sustainable and economic viable sources for biodiesel production