A Whole Virus Pandemic Influenza H1N1 Vaccine Is Highly Immunogenic and Protective in Active Immunization and Passive Protection Mouse Models

The recent emergence and rapid spread of a novel swine-derived H1N1 influenza virus has resulted in the first influenza pandemic of this century. Monovalent vaccines have undergone preclinical and clinical development prior to initiation of mass immunization campaigns. We have carried out a series of immunogenicity and protection studies following active immunization of mice, which indicate that a whole virus, nonadjuvanted vaccine is immunogenic at low doses and protects against live virus challenge. The immunogenicity in this model was comparable to that of a whole virus H5N1 vaccine, which had previously been demonstrated to induce high levels of seroprotection in clinical studies. The efficacy of the H1N1 pandemic vaccine in protecting against live virus challenge was also seen to be equivalent to that of the H5N1 vaccine. The protective efficacy of the H1N1 vaccine was also confirmed using a severe combined immunodeficient (SCID) mouse model. It was demonstrated that mouse and guinea pig immune sera elicited following active H1N1 vaccination resulted in 100% protection of SCID mice following passive transfer of immune sera and lethal challenge. The immune responses to a whole virus pandemic H1N1 and a split seasonal H1N1 vaccine were also compared in this study. It was demonstrated that the whole virus vaccine induced a balanced Th-1 and Th-2 response in mice, whereas the split vaccine induced mainly a Th-2 response and only minimal levels of Th-1 responses. These data supported the initiation of clinical studies with the same low doses of whole virus vaccine that had previously been demonstrated to be immunogenic in clinical studies with a whole virus H5N1 vaccine

Genetic and environmental influences on the covariance of facets defining the domains of the five-factor model of personality

Jang KL, Livesley WJ, Angleitner A, Riemann R, Vernon PA. Genetic and environmental influences on the covariance of facets defining the domains of the five-factor model of personality. PERSONALITY AND INDIVIDUAL DIFFERENCES. 2002;33(1):83-101.Multivariate genetic analyses were applied to the six facets defining each of the five personality domains (Neuroticism, Extraversion, Openness to Experience, Agreeableness, and Conscientiousness) assessed by Costa and McCrae's Revised NEO Personality Inventory (NEO-PI-R). The analyses are designed to partition the observed covariance of facets defining each domain into their genetic and environmental bases to determine the basis for their coherence as a domain. The analyses were applied separately to a sample of 253 identical and 207 fraternal twin pairs from Canada and 526 identical and 269 fraternal pairs from Germany. Results showed that each of the NEO-PI-R domains is composed of multiple genetic and environmental factors common to the facets supporting the observed coherence of the NEO-PI-R facet sets. Differences between the German and Canadian sample appeared limited to the magnitude of the genetic and environmental effects on each facet, but not the number or type of genetic and environmental influences. (C) 2002 Elsevier Science Ltd. All rights reserved

Covariance structure of neuroticism and agreeableness: A twin and molecular genetic analysis of the role of the serotonin transporter gene

Jang KL, Hu S, Livesley WJ, et al. Covariance structure of neuroticism and agreeableness: A twin and molecular genetic analysis of the role of the serotonin transporter gene. JOURNAL OF PERSONALITY AND SOCIAL PSYCHOLOGY. 2001;81(2):295-304.The Revised,NEO Personality Inventory domains of Neuroticism and Agreeableness are considered factorially distinct despite several intercorrelations between these. domains. The genetic correlation, an index of the degree to which these intercorrelations are caused by genetic influences, was estimated using data from 913 monozygotic and 562 dizygotic volunteer twin pairs from Canada, Germany, and Japan. The serotonin transporter gene, 5-HTTLPR, was assayed in a sample of 388 nontwin sibling pairs from the United States to determine the contribution of the serotonin transporter locus to the covariation between the Neuroticism and Agreeableness scales. In all four samples, genetic influences contributed to the covariance of Neuroticism and Agreeableness, with the serotonin transporter gene accounting for 10% of the relationship between these domains