Cohort profile: the 100 million Brazilian cohort

The creation of The 100 Million Brazilian Cohort was motivated by the availability of high quality but dispersed social and health databases in Brazil and the need to integrate data and evaluate the impact of policies aiming to improve the social determinants of health (e.g. social protection policies) on health outcomes, overall and in subgroups of interest in a dynamic cohort. • The baseline of The 100 Million Brazilian Cohort comprises 131 697 800 low-income individuals in 35 358 415 families from 2011 to 2018. The Cohort population is mostly composed of children and young adults, with a higher proportion of females than the general Brazilian population, who identify themselves as Brown and live in the urban area of the country. • Exposure to social protection and the follow-up of individuals are obtained through: (i) deterministic linkage using the Social Identification Number (NIS) to link the Cohort baseline to social protection programmes and to periodically renewed socioeconomic information in Cadatro U ́ nico datasets; and/or (ii) non-deterministic linkage using the CIDACS-RL non-deterministic linkage tool, to link the Cohort baseline to administrative health care datasets such as mortality (Mortality Information System, SIM), disease notification (Information System for Notifiable Diseases, SINAN), birth information (Live Birth Information System, SINASC) and nutrition status (Food and Nutrition Surveillance System, SISVAN). • So far, studies have used The 100 Million Brazilian Cohort to investigate the socioeconomic and demographic determinants of leprosy, leprosy treatment outcomes and low birthweight and to evaluate the impact of the Bolsa Familia Programme (BFP) on leprosy and child mortality. Other studies are now being conducted that are of utmost relevance to the health inequalities of Brazil and many low- and middle-income countries, and many research opportunities are being opened up with the linkage of a range of health outcomes

Evapotranspiration of rose cultivated in protected environment

The objective of this study was to determine the evapotranspiration and crop coefficient for the cut rose (cv. Carola) cultivated in protected environment and the coefficient of small tank installed inside the greenhouse during the period of one year. Five rows of plants were planted with spacing of 1.20 m between rows and 0.20 m between plants. Each line consisted of five plots with five plants per plot. Plants of the second and fourth rows and of the second and fourth plots were installed in four drainage lysimeters with dimensions of 1.20 x 1.20 x 0.80 m. The plants of the remaining plots were considered as border plants. A drip irrigation system was used. An automatic weather station was installed in the greenhouse and the data were collected and stored daily at an interval of one hour in a data-logger. The crop coefficient (Kc) showed mean values of 0.75 in the vegetative phase and 1.18 during the productive phase. The small pan (tank) coefficient (Ktr) was 0.72

Phytochemical screening of the dichloromethaneethanolic extract of Eriosema campestre var. macrophylum roots and its antiproliferative effect on human peripheral blood lymphocytes.

Eriosema campestre var. macrophylum (Grear) Fortunato, Fabaceae, is a native plant of the Brazilian Cerrado and the decoction of its roots has been used by folk medicine for the therapy of inflammatory diseases. In this study we aimed to investigate the effect of the dichloromethane-ethanolic extract of E. campestre roots on the proliferative response of lymphocytes and to examine the profile of IL-2 production. The effect of dichloromethane-ethanolic extract of E. campestre on the proliferation of phytohemagglutinin-stimulated lymphocytes was evaluated by using flow cytometry and the cell supernatants were assayed for IL-2 concentrations by using an enzyme-linked immunosorbent assay. The phytochemical screening of E. campestre roots was performed to determine the main secondary metabolites through chromogenic and precipitation reactions and by using HPLC-PAD. In addition to the presence of subclasses of flavonoids (flavones and flavonols) in dichloromethaneethanolic extract of E. campestre, we observed that the extract induced a concentration-dependent decrease in IL-2 levels on the supernatant of the cell cultures as well as an antiproliferative effect on T lymphocytes, including CD4+ and CD8+ cells. The anti-inflammatory effects attributed to E. campestre by folk medicine may partly be explained by its antiproliferative action on T lymphocytes. (C) 2016 Sociedade Brasileira de Farmacognosia. Published by Elsevier Editora Ltda.FAPEMIGCNPqCAPESUniv Fed Vales Jequitinhonha & Mucuri, Dept Farm, Lab Imunol, Diamantina, MG, BrazilUniv Fed Vales Jequitinhonha & Mucuri, Inst Ciencia & Tecnol, Diamantina, MG, BrazilUniv Fed Vales Jequitinhonha & Mucuri, Lab Farmacognosia, Diamantina, MG, BrazilUniv Fed Sao Paulo, Lab Ciencias Ambientais Quim & Farmaceut, Diadema, SP, BrazilUniv Fed Sao Paulo, Lab Ciencias Ambientais Quim & Farmaceut, Diadema, SP, BrazilFAPEMIG: CBB-APQ-00581-11Web of Scienc