Developmental expression of esophageal gland antigens and their detection in stylet secretions of Meloidogyne incognita

La microscopie en immunofluorescence a été utilisée avec des anticorps monoclonaux pour montrer que la production d'antigènes dans les glandes oesophagiennes de #Meloidogyne incognita est régulée par le développement durant la phase parasite et que quelques-uns de ces antigènes peuvent être excrétés au travers du stylet. Plusieurs antigènes des glandes oesophagiennes sont temporairement exprimés dans les glandes subventrales et dorsale des juvéniles préparasites de deuxième stade (J2), des juvéniles parasites (J2) et des femelles adultes. Deux anticorps monoclonaux reconnaissant les glandes subventrales de J2 et un anticorps monoclonal reconnaissant la glande dorsale et les glandes subventrales de J2, reconnaissent également les sécrétions passant au travers du stylet des J2. Deux anticorps monoclonaux reconnaissant la glande dorsale des femelles adultes, et un anticorps monoclonal reconnaissant leurs glandes subventrales, reconnaissent aussi les sécrétions passant au travers du stylet des femelles adultes. Ces résultats montrent que les sécrétions des glandes dorsale et subventrales peuvent passer au travers du stylet de #M. incognita et que leur expression est régulée par le développement. (Résumé d'auteur

Immunocytochemical analysis of the stage-specific distribution of collagen in the cuticle of Meloidogyne incognita

Il a été porduit un antisérum polyclonal dirigé contre la protéine majeure du collagène (76 kDa) extraite de la cuticule de femelles adultes de #Meloidogyne incognita. La composition en acides aminés de cette protéine est semblable à celle du collagène d'autres nématodes. Parmi les protéines extraites de la cuticule de femelles adultes de #Meloidogyne et solubles dans le bêta-mercaptoéthanol, deux protéines du collagène (Mr 76 et 140 kDa) ainsi que plusieurs protéines du collagène de junéviles de deuxième stade (J2), réagissent au cours d'analyse Western blot avec des anticorps polyclonaux. L'antisérum polyclonal a été utilisé en microscopie électronique pour localiser le collagène dans la cuticule de différents stades et dans la paroi de l'oeuf. Chez les J2 libres et les adultes mâles, où la cuticule est formée de trois couches distinctes, un marquage, intense, par l'or ne concerne que la couche corticale, et non l'épicuticule, la couche médiane ou la couche basale striée. Chez les J2 sédentaires enflés, dont la cuticule est de structure homogène, les particules d'or sont réparties dans toute la profondeur de la cuticule. Les particules d'or sont également uniformément réparties dans toute l'épaisseur de la cuticule des femelles adultes, y compris après purification au SDS. Dans la paroi de l'oeuf, le marquage à l'or est visible à la surface de la couche chitineuse. (Résumé d'auteur

Enhancing Cybersecurity Content in Undergraduate Information Systems Programs: A Way Forward

The ongoing barrage of data and infrastructure breaches is a constant reminder of the critical need to enhance the cybersecurity component of modern undergraduate information systems (IS) education. Although the most recent undergraduate information systems curricular guidelines (IS2010) highlight security in the context of data, enterprise architecture, and risk management, much more needs to be done. The IS education community needs to identify cybersecurity competencies and curricular content that further integrates cybersecurity principles and practices into IS curricular guidelines. Until this is completed at the IS community level, IS programs will need to fulfill this role individually. This paper contributes to both these efforts by reviewing relevant literature and initiatives – highlighting two primary paths of curricular development: (1) the evolution of IS curricular guidelines, and (2) the development of Cybersecurity as a standalone discipline. Using these resources, the paper summarizes best practices for integrating cybersecurity into curricula and explores the integration of IS into cybersecurity programs

Particle motion and stain removal during simulated abrasive tooth cleaning

Stain removal from teeth is important both to prevent decay and for appearance. This is usually achieved using a filament-based toothbrush with a toothpaste consisting of abrasive particles in a carrier fluid. This work has been carried out to examine how these abrasive particles interact with the filaments and cause material removal from a stain layer on the surface of a tooth. It is important to understand this mechanism as while maximum cleaning efficiency is required, this must not be accompanied by damage to the enamel or dentine substrate. In this work simple abrasive scratch tests were used to investigate stain removal mechanism of two abrasive particles commonly used in tooth cleaning, silica and perlite. Silica particles are granular in shape and very different to perlite particles, which are flat and have thicknesses many times smaller than their width. Initially visualisation studies were carried out with perlite particles to study how they are entrained into a filament/counterface contact. Results were compared with previous studies using silica. Reciprocating scratch tests were then run to study how many filaments have a particle trapped at one moment and are involved in the cleaning process. Stain removal tests were then carried out in a similar manner to establish cleaning rates with the two particle types. Perlite particles were found to be less abrasive than silica. This was because of their shape and how they were entrained into the filament contacts and loaded against a counterface. With both particles subsurface damage during stain removal was found to be minimal. A simple model was built to predict stain removal rates with silica particles, which gave results that correlated well with the experimental data

Relativistic two-body equation based on the extension of the SL(2,C) group

A new approach to the two-body problem based on the extension of the $SL(2,C)$ group to the $Sp(4,C)$ one is developed. The wave equation with various forms of including the interaction for the system of the spin-1/2 and spin-0 particles is constructed. For this system, it was found that the wave equation with a linear confinement potential involved in the non-minimal manner has an oscillator-like form and possesses the exact solution.Comment: 9 pages, no figure

Pinned Balseiro-Falicov Model of Tunneling and Photoemission in the Cuprates

The smooth evolution of the tunneling gap of Bi_2Sr_2CaCu_2O_8 with doping from a pseudogap state in the underdoped cuprates to a superconducting state at optimal and overdoping, has been interpreted as evidence that the pseudogap must be due to precursor pairing. We suggest an alternative explanation, that the smoothness reflects a hidden SO(N) symmetry near the (pi,0) points of the Brillouin zone (with N = 3, 4, 5, or 6). Because of this symmetry, the pseudogap could actually be due to any of a number of nesting instabilities, including charge or spin density waves or more exotic phases. We present a detailed analysis of this competition for one particular model: the pinned Balseiro-Falicov model of competing charge density wave and (s-wave) superconductivity. We show that most of the anomalous features of both tunneling and photoemission follow naturally from the model, including the smooth crossover, the general shape of the pseudogap phase diagram, the shrinking Fermi surface of the pseudogap phase, and the asymmetry of the tunneling gap away from optimal doping. Below T_c, the sharp peak at Delta_1 and the dip seen in the tunneling and photoemission near 2Delta_1 cannot be described in detail by this model, but we suggest a simple generalization to account for inhomogeneity, which does provide an adequate description. We show that it should be possible, with a combination of photoemission and tunneling, to demonstrate the extent of pinning of the Fermi level to the Van Hove singularity. A preliminary analysis of the data suggests pinning in the underdoped, but not in the overdoped regime.Comment: 18 pages LaTeX, 26 ps. figure

Environmental baseline monitoring - Vale of Pickering: Phase I - final report (2015/16)

This report presents the collated results from the BGS-led project Science-based environmental baseline monitoring associated with shale gas development in the Vale of Pickering (including supplementary air quality monitoring in Lancashire). The project has been funded by a grant awarded by DECC for the period August 2015 – 31st March 2016. It complements (and extends to air quality) an on-going project, funded by BGS and the other project partners, in which similar activities are being carried out in the Fylde area of Lancashire. The project has initiated a wide-ranging environmental baseline monitoring programme that includes water quality (groundwater and surface water), seismicity, ground motion, atmospheric composition (greenhouse gases and air quality), soil gas and radon in air (indoors and outdoors). The motivation behind the project(s) was to establish independent monitoring in the area around the proposed shale gas hydraulic fracturing sites in the Vale of Pickering, North Yorkshire (Third Energy) and in Lancashire (Cuadrilla) before any shale gas operations take place. As part of the project, instrumentation has been deployed to measure, in real-time or near real-time, a range of environmental variables (water quality, seismicity, atmospheric composition). These data are being displayed on the project’s web site (www.bgs.ac.uk/Valeofpickering). Additional survey, sampling and monitoring has also been carried out through a co-ordinated programme of fieldwork and laboratory analysis, which has included installation of new monitoring infrastructure, to allow compilation of one of the most comprehensive environmental datasets in the UK. It is generally recognised that at least 12 months of baseline data are required. The duration of the grant award (7 months) has meant that this has not yet been possible. However there are already some very important findings emerging from the limited datasets which need be taken in to account when developing future monitoring strategy, policy and regulation. The information is not only relevant to the Vale of Pickering and Lancashire but will be more widely applicable in the UK and internationally. Although shale gas operations in other parts of the world are well-established there is a paucity of good baseline data and effective guidance on monitoring. It is hoped that the monitoring project will continue to allow at least 12 months of data for each of the work packages to be compiled and analysed. It will also allow the experience gained and the scientifically-robust findings to be used to develop and establish effective environmental monitoring strategies for shale gas and similar industrial activities

Barriers to development and progression of women entrepreneurs in Pakistan

This article would help integration of women entrepreneurs into the mainstream economy in Pakistan.In Pakistan, women entrepreneurs do not enjoy the same opportunities as men due to a number of deep-rooted discriminatory socio-cultural values and traditions. Furthermore, these restrictions can be observed within the support mechanisms that exist to assist such fledgling businesswomen. The economic potential of female entrepreneurs is not being realised as they suffer from a lack of access to capital, land, business premises, information technology, training and agency assistance. Inherent attitudes of a patriarchal society, that men are superior to women and that women are best suited to be homemakers, create formidable challenges. Women also receive little encouragement from some male family members, resulting in limited spatial mobility and a dearth of social capital. The research suggests that in order to foster development, multi-agency cooperation is required. The media, educational policy makers and government agencies could combine to provide women with improved access to business development services and facilitate local, regional and national networks

Tunneling conductance of SIN junctions with different gap symmetries and non-magnetic impurities by direct solution of real-axis Eliashberg equations

We theoretically investigate the effect of various symmetries of the superconducting order parameter Delta(omega) on the normalized tunneling conductance of SIN junctions by directly solving the real-axis Eliashberg equations (EEs) for a half-filled infinite band, with the simplifying assumption mu*=0. We analyze six different symmetries of the order parameter: s, d, s+id, s+d, extended s and anisotropic s, by assuming that the spectral function alpha^{2}F(Omega) contains an isotropic part alpha^{2}F(Omega)_{is} and an anisotropic one, alpha^{2}F(Omega)_{an}, such that alpha^{2}F(Omega)_{an} = g alpha^{2}F(Omega)_{is}, where g is a constant. We compare the resulting conductance curves at T=2 K to those obtained by analytical continuation of the imaginary-axis solution of the EEs, and we show that the agreement is not equally good for all symmetries. Then, we discuss the effect of non-magnetic impurities on the theoretical tunneling conductance curves at T=4 K for all the symmetries considered. Finally, as an example, we apply our calculations to the case of optimally-doped high-T_{c} superconductors (HTSC). Surprisingly, although the possibility of explaining the very complex phenomenology of HTSC is probably beyond the limits of the Eliashberg theory, the comparison of the theoretical curves calculated at T=4 K with the experimental ones obtained in various optimally-doped copper-oxides gives fairly good results.Comment: 11 pages, 11 figures, 2 tables. References added, figs. 6,7,10 and 11 changed, text change

Interaction between toothbrushes and toothpaste abrasive particles in simulated tooth cleaning

There are currently many toothbrush designs on the market incorporating different filament configurations such as filaments at various angles and different lengths and made from several different materials. In order to understand how the tooth cleaning process occurs there is a need to investigate in detail how the abrasive particles in a toothpaste interact with the filaments in a teeth cleaning contact and cause material removal from a plaque or stain layer. The following describes the development of optical apparatus to enable the visualisation of simulated teeth cleaning contacts. Studies have been carried out using the apparatus to investigate particle entrainment into the contact and how it differs with varying bristle configurations. The effects of filament stiffness and tip shape were also investigated. Various types of electric toothbrushes were also tested. The studies have shown how particles are trapped at the tips of toothbrush filaments. Particles, suspended in fluid, approach the filament tips, as they pass through they may become trapped. Greater particle entrainment into the filament tip contact occurs with a reciprocating action at low filament loads and deflections than with a sliding motion. Large particles are less likely to enter tip contacts and are trapped between tips or under the filament bend at higher loads. Whether the particles are likely to be trapped and how long they remain so depends on the filament stiffness and degree of splay on loading and the filament configuration. The direction the filaments point in, the number of filaments in a tuft, the spacing of the tufts and the way the filaments splay when deflected all have an influence on entrainment of particles. Tufts with tightly packed stiff filaments which deflected together on loading were more effective at trapping particles than more flexible filaments that splayed out on loading as they present more of a barrier to particle entry and exit from the tip region