PROTECTIVE EFFECT OF ONION EXTRACT AGAINST EXPERIMENTAL IMMUNESUPPRESSION IN WISTAR RATS: BIOLOGICAL AND MOLECULAR STUDY

Background: The wrong use of drugs results in disturbances in the immunity that affect human health. These drugs have side effects that may lead to death because of lake of immunity. Human beings need to use natural products to strength the immune system and avoid such side effects. Of these products is the onion that used to strength the immune system. This study was conducted to study the protective effect of onion extract on immune-suppressed rats and its impact on the expression level of cytokines, acute phase proteins and antioxidants compared to both control and immune suppressed groups. Materials and Methods: Forty rats were divided into four groups (10 per group) control group (CNT), immunesuppressed group (DEXA) injected with dexamethasone at a dose of 5 mg per kg intraperitoneally (IP) twice daily for 3 days, onion extract administered group (OE) given orally at a dose of 500 mg per kg for 4 weeks. Group 4 (y), was given onion extract for a week then immune-suppressed with DEXA for 3 days then continued with OE for 3 weeks. Serum and RNA were extracted for examining the biochemical and genetic changes. Results: Injection of dexamethasone decreased number of leukocytes with increase in the number of neutrophils and the decrease of all other types of white blood cells. Moreover, a decrease in antioxidant levels such as catalase, super oxide dismutase (SOD), and reduced glutathione (GSH) with an increase in the level malondialdehyde (MDA). In parallel, a decrease in serum levels of cytokines, such as TNF and IL-6, together with immunoglobulins (IgG and IgM), were reported in DEXA injected rats that were ameliorated by prior administration of OE. Gene expression analysis revealed that dexamethasone suppressed gene expression of antioxidants together with IL-1 and 8 while increased IL- 10 mRNA expression. All of these changes have been normalized to the normal level by OE administration to DEXA injected rats. Conclusion: The present findings clearly emphasize the medical importance of onions as immune-stimulants at genetic and cellular levels, and that they are good for human health

Disposition of Cefquinome in Turkeys (Meleagris gallopavo) Following Intravenous and Intramuscular Administration

The bioavailability and pharmacokinetics in turkeys of cefquinome (CFQ), a broad-spectrum 4th-generation cephalosporin antibiotic, were explored after a single injection of 2 mg/kg body weight by intravenous (IV) and intramuscular (IM) routes. In a crossover design and 3-weeks washout interval, seven turkeys were assigned for this objective. Blood samples were collected prior to and at various time intervals following each administration. The concentration of CFQ in plasma was measured using HPLC with a UV detector set at 266 nm. For pharmacokinetic analysis, non-compartmental methods have been applied. Following IV administration, the elimination half-life (t1/2ʎz), distribution volume at steady state (Vdss), and total body clearance (Cltot) of CFQ were 1.55 h, 0.54 L/kg, and 0.32 L/h/kg, respectively. Following the IM administration, CFQ was speedily absorbed with an absorption half-life (t1/2ab) of 0.25 h, a maximum plasma concentration (Cmax) of 2.71 μg/mL, attained (Tmax) at 0.56 h. The bioavailability (F) and in vitro plasma protein binding of CFQ were 95.56% and 11.5%, respectively. Results indicated that CFQ was speedily absorbed with a considerable bioavailability after IM administration. In conclusion, CFQ has a favorable disposition in turkeys that can guide to estimate optimum dosage regimes and eventually lead to its usage to eradicate turkey’s susceptible bacterial infections

Disposition of Cefquinome in Turkeys (<i>Meleagris gallopavo</i>) Following Intravenous and Intramuscular Administration

The bioavailability and pharmacokinetics in turkeys of cefquinome (CFQ), a broad-spectrum 4th-generation cephalosporin antibiotic, were explored after a single injection of 2 mg/kg body weight by intravenous (IV) and intramuscular (IM) routes. In a crossover design and 3-weeks washout interval, seven turkeys were assigned for this objective. Blood samples were collected prior to and at various time intervals following each administration. The concentration of CFQ in plasma was measured using HPLC with a UV detector set at 266 nm. For pharmacokinetic analysis, non-compartmental methods have been applied. Following IV administration, the elimination half-life (t1/2ʎz), distribution volume at steady state (Vdss), and total body clearance (Cltot) of CFQ were 1.55 h, 0.54 L/kg, and 0.32 L/h/kg, respectively. Following the IM administration, CFQ was speedily absorbed with an absorption half-life (t1/2ab) of 0.25 h, a maximum plasma concentration (Cmax) of 2.71 μg/mL, attained (Tmax) at 0.56 h. The bioavailability (F) and in vitro plasma protein binding of CFQ were 95.56% and 11.5%, respectively. Results indicated that CFQ was speedily absorbed with a considerable bioavailability after IM administration. In conclusion, CFQ has a favorable disposition in turkeys that can guide to estimate optimum dosage regimes and eventually lead to its usage to eradicate turkey’s susceptible bacterial infections

Drought-Induced Oxidative Stress in Pearl Millet (Cenchrus americanus L.) at Seedling Stage: Survival Mechanisms through Alteration of Morphophysiological and Antioxidants Activity

We report the impact of drought stress on pearl millet during the early seedling stage and its survival mechanism. Drought stress imposed for a period of 7, 14 and 21 days showed considerable changes in morphophysiological attributes, which were evident by a decline in seedling elongation, fresh and dry biomass, and relative water content (RWC) and degradation of chlorophyll pigment. Besides this, visible chlorosis lesions were observed in leaves as compared to the control. As compared to the respective controls, a nearly 60% decline in chlorophyll content was recorded after 14 and 21 days of drought stress. In both root and shoot, drought stress raised the reactive oxygen species (ROS) levels. Both H2O2 and O2&#9679;&minus; levels were significantly elevated along with a significant increase in lipid peroxidation in both roots and shoots, which clearly indicated ROS-induced oxidative stress. Concomitant with the increase in ROS levels and malondialdehyde (MDA) content in roots, membrane integrity was also lost, which clearly indicated ROS-induced peroxidation of membrane lipids. The activities of antioxidant enzymes and levels of non-enzymatic antioxidants were significant (p &le; 0.001). After 7, 14 and 21 days of drought stress, activities of all the antioxidant enzymes viz., catalase (CAT), guaiacol peroxidase (GPX), superoxide dismutase (SOD) and glutathione reductase (GR) were inhibited, clearly indicating a loss of antioxidant defense machinery. Likewise, the levels of ascorbate (AsA) and reduced glutathione (GSH) levels declined significantly (p &le; 0.01). Our results reveal that, being tolerant to arid climatic conditions, pearl millet is highly susceptible to drought stress at the early seedling stage

Enhancement Effects of Water Magnetization and/or Disinfection by Sodium Hypochlorite on Secondary Slaughterhouse Wastewater Effluent Quality and Disinfection By-Products

Wastewater disinfection is one of the most critical issues in protecting human health against exposure to waterborne pathogenies. Chlorine is among the most commonly used disinfectants in many wastewaters&rsquo; treatment plants. Nevertheless, disquiets regarding chlorine&rsquo;s disinfection by-products (DBPs) have grown recently. One of the most effective ways to reduce DBPs generation is to reduce chlorine dosage by increasing disinfectant efficiency. Using magnetic field (MF) in wastewater treatment is one of the promising research topics with significant progression. This study aimed to evaluate the efficiency of using a magnetic field and/or sodium hypochlorite (NaClO) disinfection on secondary slaughterhouse wastewater effluent quality and by-products. Three groups of secondary slaughterhouse wastewater effluents were used: G1 was treated with NaClO only at 0, 2, 4, and 6 mg/L; G2 was treated with exposure to MF at 14,500 gausses, and G3 was pretreated with MF, then NaClO at the exact chlorine dosages and MF strength. The results showed an augmented effect when using a magnetic field as a pre-treatment step before NaClO treatment in the remediation of slaughterhouse wastewater over the use of any of them solely. The removal rate of COD and BOD increased by up to 26 and 20%, respectively, when pre-treatment with MF was employed as a mean percentage at all chlorine dosages, while TSS, TDS, and EC increased by 23.5 and 5.5%, respectively. Over and above, the removal rate for each TN and TP increased by 12 and 6.5% as a mean percentage at all chlorine dosages when using a combination of the two. In addition, pre-treatment by MF reduced the required concentration of NaClO from 6 to 4 mg/L, resulting in an 11% increase in the reduction rate of total coliform count, 8% increase in the reduction rate of fecal coliforms, and 10% increase in the reduction rate of E. coli and 5% in Salmonella via increasing the disinfection efficiency of NaClO. Finally, it decreased the concentration of Chloroform produced by more than 77.2% by using the higher concentration of NaClO (6 mg/L). The issue that approved the promising approach of using MF as a pre-treatment step in the treatment of slaughterhouse wastewater provides the advantage of using smaller dosages of disinfection, lowering the cost of the procedure process, and reducing the harmful concentration of DBPs

Protective Impacts of Moringa oleifera Leaf Extract against Methotrexate-Induced Oxidative Stress and Apoptosis on Mouse Spleen

Objective. The current study was aimed to examine the possible ameliorative impacts of MO leaf extract (MOLE) against MTX-induced alterations on oxidative stress of mouse spleen and explore the possible molecular mechanism that controls such impacts. Methods. Adult male mice were allocated into 4 groups: control, Moringa oleifera leaf extract (MOLE), MTX, and MOLE plus MTX. Mice received MOLE orally for a week before MTX injection and continued for 12 days. Serum and spleen were sampled for biochemical and quantitative gene expressions. Results. As compared with the MTX-injected group, MOLE effectively reduced the changes in total proteins, spleen MDA, SOD and catalase activities, and changes in serum antioxidants levels. Moreover, there is downregulation of antioxidant genes (SOD and catalase) and antiapoptotic genes (XIAP and Bcl-xl) along with upregulation in Bax and caspase-3 mRNA (apoptotic genes) in the MTX-injected group. MTX induced changes in IL-1β, IL-6, TNF-α, and IL-10 expression. MOLE restored and ameliorated the changes induced in biochemical, antioxidants, apoptosis, and apoptosis associated genes that were induced by MTX intoxication. Conclusion. Current findings indicated that pretreatment with MOLE to MTX-intoxicated mice showed the potential usage of MO for oxidative stress and apoptosis treatment

Prevalence and Characterization of PVL-Positive <i>Staphylococcus aureus</i> Isolated from Raw Cow’s Milk

The present study aimed to investigate the prevalence, antibiotic susceptibility profiles, and some toxin genes of Panton-Valentine leukocidin (PVL)-positive Staphylococcus aureus (S. aureus) in unpasteurized raw cow’s milk collected from retail outlets located at Mansoura, Dakahliya governorate, Egypt. In that context, a total of 700 raw cow’s milk samples were investigated for the presence of S. aureus, which was identified in 41.1% (288/700) of the samples. Among the S. aureus isolates, 113 PVL-positive S. aureus were identified and subjected for further analysis. The PVL-positive S. aureus were investigated for the existence of toxin-related genes, including hemolysin (hla), toxic shock syndrome toxin-1 (tst), and enterotoxins (sea, seb, sec, see, seg, sei, and selj). Genotypic resistance of PVL-positive strains was performed for the detection of blaZ and mecA genes. Among the PVL-positive S. aureus, sea, seb, and sec were detected in 44.2, 6.2%, and 0.9%, respectively, while the hla and tst genes were identified in 54.9% and 0.9%, respectively. The blaZ and mecA genes were successfully identified in 84.9 (96/113) and 32.7% (37/113) of the total evaluated S. aureus isolates, respectively. PVL-positive S. aureus displayed a high level of resistance to penicillin, ampicillin, and trimethoprim-sulfamethoxazole. Multidrug resistance (resistant to ≥3 antimicrobial classes) was displayed by all methicillin-resistant S. aureus (MRSA) and 38.2% of methicillin-sensitive S. aureus (MSSA) isolates. The obtained findings are raising the alarm of virulent PVL-positive MRSA clones in retail milk in Egypt, suggesting the requirement for limiting the use of β-lactam drugs in food-producing animals and the importance of implementing strong hygiene procedures in dairy farms and processing plants

The effects of paulownia (Paulownia tomentosa) leaf extract enriched diets on meat quality, sensory attributes, and the potential economic impact of broilers

The present study was conducted to assess the effect of paulownia (Paulownia tomentosa) leaf extract (PLE) enriched diets on the quality of meat and the economic efficiency of broiler chickens. A total of 180 one-day-old male chicks (Cobb) were randomly assigned to four treatments with five replications each (9 birds per replicate). The chickens were fed corn-soybean-based diets supplemented with different levels of PLE (0, 0.1, 0.3, and 0.5 g kg−1) for 42 days. The results showed that the inclusion of up to 0.5 g kg−1 PLE in the diet of broiler chickens significantly improved the live body weight, carcase weight, and carcase components (liver, heart, and gizzard) weights compared to the control group (P < 0.05). Enriched broiler diets with 0.5 g kg−1 of PLE significantly reduced collagen and lipid content as well as increased total protein levels in both breast and thigh muscle compared to the un-supplemented group (P < 0.001). Subjective evaluation of the breast meat showed a significant linear improvement in flavour and juiciness of meat samples from birds fed with dietary PLE in a dose-dependent manner (P < 0.05). Supplementation with different levels of PLE significantly improved the sensory attributes (flavour, tenderness, juiciness, and overall acceptability) of thigh meat in a dose-dependent manner (linear; P < 0.05). Total potential return and net profit were significantly increased in all groups fed PLE compared to the control group. Birds that received PLE-supplemented diets at a level of 0.5 g kg−1 had the highest economic efficiency (P < 0.05). Conclusively, supplementation with 0.5 g PLE/kg in broiler diets could improve meat quality and economic efficiency.Highlights Enriched broiler diets with 0.5 g paulownia leaf extract significantly enhanced carcase measurements. Supplemented broiler diets with 0.5 g PLE significantly enhanced the meat quality and sensory attributes of thigh meat. Birds fed PLE-supplemented diets at a level of 0.5 g/kg had the highest economic efficiency