Re-annotation of the woodland strawberry (Fragaria vesca) genome

Fragaria vesca is a low-growing, small-fruited diploid strawberry species commonly called woodland strawberry. It is native to temperate regions of Eurasia and North America and while it produces edible fruits, it is most highly useful as an experimental perennial plant system that can serve as a model for the agriculturally important Rosaceae family. A draft of the F. vesca genome sequence was published in 2011 [Nat Genet 43:223,2011]. The first generation annotation (version 1.1) were developed using GeneMark-ES+[Nuc Acids Res 33:6494,2005]which is a self-training gene prediction tool that relies primarily on the combination of ab initio predictions with mapping high confidence ESTs in addition to mapping gene deserts from transposable elements. Based on over 25 different tissue transcriptomes, we have revised the F. vesca genome annotation, thereby providing several improvements over version 1.1. The new annotation, which was achieved using Maker, describes many more predicted protein coding genes compared to the GeneMark generated annotation that is currently hosted at the Genome Database for Rosaceae (http://www.rosaceae.org/). Our new annotation also results in an increase in the overall total coding length, and the number of coding regions found. The total number of gene predictions that do not overlap with the previous annotations is 2286, most of which were found to be homologous to other plant genes. We have experimentally verified one of the new gene model predictions to validate our results. Using the RNA-Seq transcriptome sequences from 25 diverse tissue types, the re-annotation pipeline improved existing annotations by increasing the annotation accuracy based on extensive transcriptome data. It uncovered new genes, added exons to current genes, and extended or merged exons. This complete genome re-annotation will significantly benefit functional genomic studies of the strawberry and other members of the Rosaceae.https://doi.org/10.1186/s12864-015-1221-

ВЛИЯНИЕ ВЗАИМООТНОШЕНИЙ МЕЖДУ ТРЕНЕРОМ И СПОРТСМЕНОМ НА УРОВЕНЬ МОТИВАЦИИ ДОСТИЖЕНИЯ СПОРТСМЕНА

The purpose of the study was to study the influence of the relationship between a coach and an athlete on the level of motivation of an athlete's achievement. The sample consisted of 64 athletes (girls and boys) aged 13 to 18 years, engaged in the Syrian Sports Federation in Latakia in such sports as athletics, football, table tennis, tennis, boxing and gymnastics. A descriptive approach was used as a methodology due to its relevance to the nature of the study, an author's questionnaire was used to assess the relationship in the coach-athlete system, and a methodology was used to assess the motivation for achievement (Abdel Karim and Barakat, 2007). The data were analyzed using statistical methods: arithmetic averages, standard deviations, Pearson correlation coefficient. The results of the study revealed that there are reliable links between the relationship in the "coach-athlete" system with the level of motivation of an athlete's achievement.Цель исследования заключалась в изучении влияния взаимоотношений между тренером и спортсменом на уровень мотивации достижения спортсмена. Выборка состояла из 64 спортсменов (девушек и юношей) в возрасте от 13 до 18 лет, занимающихся в Сирийской спортивной федерации в Латакии по таким видам спорта как легкая атлетика, футбол, настольный теннис, теннисом, бокс и гимнастика. В качестве методологии использовался описательный подход из-за его релевантности характеру исследования, для оценки отношений в системе «тренер–спортсмен» использовалась авторская анкета, для оценки мотивации достижения методика (Abdel Karim и Barakat, 2007). Данные анализировались с помощью статистических методов: средние арифметические, стандартные отклонения, коэффициент корреляции Пирсона. Результаты исследования выявили, что существуют достоверные связи между взаимоотношениями в системе «тренер–спортсмен» с уровнем мотивации достижения спортсмена

An efficient annotation and gene-expression derivation tool for Illumina Solexa datasets

<p>Abstract</p> <p>Background</p> <p>The data produced by an Illumina flow cell with all eight lanes occupied, produces well over a terabyte worth of images with gigabytes of reads following sequence alignment. The ability to translate such reads into meaningful annotation is therefore of great concern and importance. Very easily, one can get flooded with such a great volume of textual, unannotated data irrespective of read quality or size. CASAVA, a optional analysis tool for Illumina sequencing experiments, enables the ability to understand INDEL detection, SNP information, and allele calling. To not only extract from such analysis, a measure of gene expression in the form of tag-counts, but furthermore to annotate such reads is therefore of significant value.</p> <p>Findings</p> <p>We developed TASE (Tag counting and Analysis of Solexa Experiments), a rapid tag-counting and annotation software tool specifically designed for Illumina CASAVA sequencing datasets. Developed in Java and deployed using jTDS JDBC driver and a SQL Server backend, TASE provides an extremely fast means of calculating gene expression through tag-counts while annotating sequenced reads with the gene's presumed function, from any given CASAVA-build. Such a build is generated for both DNA and RNA sequencing. Analysis is broken into two distinct components: DNA sequence or read concatenation, followed by tag-counting and annotation. The end result produces output containing the homology-based functional annotation and respective gene expression measure signifying how many times sequenced reads were found within the genomic ranges of functional annotations.</p> <p>Conclusions</p> <p>TASE is a powerful tool to facilitate the process of annotating a given Illumina Solexa sequencing dataset. Our results indicate that both homology-based annotation and tag-count analysis are achieved in very efficient times, providing researchers to delve deep in a given CASAVA-build and maximize information extraction from a sequencing dataset. TASE is specially designed to translate sequence data in a CASAVA-build into functional annotations while producing corresponding gene expression measurements. Achieving such analysis is executed in an ultrafast and highly efficient manner, whether the analysis be a single-read or paired-end sequencing experiment. TASE is a user-friendly and freely available application, allowing rapid analysis and annotation of any given Illumina Solexa sequencing dataset with ease.</p

Attitude to migration of Syrian student-athletes

Рукопись поступила в редакцию: 19.02.2023. Принята к публикации: 18.03.2023.Received: 19.02.2023. Accepted: 18.03.2023.Проблема спортивной миграции рассматривается как новое направление междисцилинарных исследований. Цель исследования заключалась в изучении отношения сирийских студентов-спортсменов к миграции в сфере спорта. Исследование проводилось среди студентов бакалавриата 1-4 курса по направлению «Физическая культура» Университета Тишрин, Латтакия, Сирия. Всего в исследовании приняло участие 34 студента, которые занимаются разными видами спорта. В качестве метода использовалась авторская анкета. Результаты, проведенного исследования выявили, что сирийские студенты спортсмены достаточно информированы о миграции спортсменов, поскольку все имеют знакомых спортсменов-мигрантов. Получены данные относительно того, какие мотивы миграции в спорте они считают наиболее значимыми, выявлено мнение студентов о трудностях миграции и стратегиях необходимых для их преодоления. Данные могут быть актуальны для специалистов занимающихся проблемой миграции в спорте.The problem of sports migration is considered as a new direction of interdisciplinary research. The purpose of the study was to study the attitudes of Syrian student athletes towards migration in the field of sports. The study was conducted among undergraduate students of 1-4 years in the faculty of “Physical culture” Tishrin University, Lattakia, Syria. A total of 34 students took part in the study. involved in different sports. The author’s questionnaire was used as a method. The results of the study revealed that Syrian student athletes are quite informed about the migration of athletes, since everyone knows migrant athletes. Data have been obtained regarding which motives for migration in sports they consider the most significant, the opinion of students about the difficulties of migration and the strategies necessary to overcome them. The data may be relevant for specialists dealing with the problem of migration in sports

The Relationship between Emotional Intelligence and Psychological States of Coaches-Instructors

Рукопись поступила в редакцию: 24.08.2024. Принята к публикации: 21.09.2024.Received: 24.08.2024. Accepted: 21.09.2024.В статье рассматривается проблема эмоционального интеллекта в профилактике эмоционального выгорания тренеров-инструкторов. Цель исследования заключалась в изучении влияния эмоционального интеллекта на психологические состояния, снижающие работоспособности тренеров-инструкторов. В исследовании приняли участие 36 тренеров-фитнес инструкторов (20 мужчин и 16 женщин), средний возраст составил 26, 3 лет. В качестве методов использовалась методика ДОРС А. Б. Леоновой и С.Б. Величковской и методика изучения эмоционального интеллекта (автор К. Т. Вонг, перевел и адаптировал К. А. Бочавер). Полученные данные свидетельствуют о том, что чем выше показатель способности к регуляции эмоциональных состояний, тем ниже уровень утомляемости и стресса у тренеров-инструкторов, в то же время чем выше уровень способности к оценке эмоций других людей, тем выше у тренеров-инструкторов уровень психических состояний монотонии и пресыщения. Полученные свидетельствуют о необходимости разработки программ по развитию эмоционального интеллекта тренеров-инструкторов, причем с акцентом на специфику их деятельности.The article deals with the problem of emotional intelligence in the prevention of emotional burnout of coaches-instructors. The purpose of the study was to study the influence of emotional intelligence on psychological conditions that reduce the performance of coaches-instructors. The study involved 36 fitness trainers (20 men and 16 women), the average age was 26.3 years. The DORS methodology of A. B. Leonova and S. B. Velichkovskaya and the methodology for studying emotional intelligence (author K. T. Wong, translated and adapted by K. A. Bochaver) were used as the methods. The obtained data indicate that the higher the indicator of the ability to regulate emotional states, the lower the level of fatigue and stress among coaches-instructors. At the same time, the higher the level of the ability to assess the emotions of other people leads to the higher the level of mental states of monotony and satiety among coaches-instructors. The results indicate the need to create programs for the development of emotional intelligence of coaches-instructors, with an emphasis on the specifics of their activities

Experience of Organizing Sports Events in Russian Universities with The Participation of Foreign Students

The article presents a Literature review about the experience of organizing sports events with the participation of foreign students. The main tasks of sports events, the features of their organization and effectiveness in the adaptation of foreign students are considered.В статье представлен аналитический обзор литературы, посвященной организации спортивных мероприятий с участием иностранных студентов. Рассмотрены основные задачи спортивных мероприятиях, особенности их организации и эффективности по адаптации иностранных студентов

A plant-feeding nematode indirectly increases the fitness of an aphid

Plants suffer multiple, simultaneous assaults from above and below ground. In the laboratory, pests and/or pathogen attack are commonly studied on an individual basis. The molecular response of the plant to attack from multiple organisms and the interaction of different defence pathways is unclear. The inducible systemic responses of the potato (Solanum tuberosum L.) host plant were analysed to characterise the plant-mediated indirect interactions between a sedentary, endoparasitic nematode (Globodera pallida) and a phloem-sucking herbivore (Myzus persicae). The reproductive success of M. persicae was greater on potato plants pre-infected with G. pallida compared to control plants. Salicylic acid (SA) increased systemically in the leaves of potato plants following nematode and aphid infection singly with a corresponding increase in expression of SA-mediated marker genes. An increase in jasmonic acid (JA) associated with aphid infection was suppressed when plants were co-infected with nematodes. Our data suggests a positive, asymmetric interaction between a sedentary endoparasitic nematode and a sap-sucking insect. The systemic response of the potato plant following infection with G. pallida indirectly influences the performance of M. persicae. This work reveals additional secondary benefits of controlling individual crop pests