Pathological Findings of Tramadol on Liver Tissue in the Cadaver Referred to Legal Medicine Organization of Tehran 2008-2013

Background: Tramadol is a synthetic opioid used to control chronic pain and pain after surgery. However, many cases of poisoning and dangerous side effects have been reported. In order to discover the causes of death in forensic medicine is usually examine tissue samples taken from the body, although the results of toxicology tests generally give us more information, but identifying the pathological effects of Tramadol on the internal organs, especially the livers can be very helpful.Methods: In this cross-sectional study, information on the history, the autopsy report and toxicology testing of patients who died due to poisoning Tramadol were studied. In this study, all of the information already provided by the use of particular forms collected and then analyzed using computer software SPSS.Results: A total of 49 cadavers were examined in this study. There were 39 dead (80%) of men and 10 of (20%) women. Most common age groups in the study were 24 to 34 years old (41%), 15 to 24 years old (39%), were reported. There were significant difference between liver microscopic spread among different ages, history of Tramadol, positive and negative toxicology tests stomach contents, urine, blood, tissue and vitreous. Most common pathological changes were in liver, degenerative changes and inflammatory cell infiltration in patients who under 35 years old.Conclusion: In this study, the majority of patients had acute poisoning with Tramadol, it can be concluded that the majority of changes in tissue obtained was as a result of acute Tramadol, respectively incidence of atelectasis and edema and alveolar bleeding in the liver were most pathological findings. In reviewing toxicology, Tramadol are also present in the stomach contents, urine, gall bladder, liver and blood were positive, the result of stomach contents will be most helpful for us

Performance, Intestinal Histomorphology, and Blood Variables of Broilers Fed Amaranth Grain in Pellet Diet

An experiment was conducted to evaluate the effects of amaranth grain in pellet diet on performance, intestinal morphology of jejunum, and selected blood variables of broilers. A total of 400 seven-day-old Ross 308 male broilers were allocated to 4 treatments with 5 replicates of 20 birds in a completely randomized design. Experimental treatments were included 4 levels of amaranth grain (0% (control), 2%, 4%, and 6%) in the isonitrogenous and isocaloric pellet diets. During the experiment, body weight (BW) and feed intake (FI) were recorded weekly and average daily gain (ADG), feed conversion ratio (FCR), as well as European broiler index (EBI), were calculated. On day 42, blood sera and jejunal tissue samples were obtained from 6 birds per replicate to evaluate morphological variables including villus height, villus width, and crypt depth, as well as selected blood variables. Although intestinal morphology and average daily feed intake (ADFI) were not influenced by experimental treatments, birds receiving 2% amaranth grain showed higher BW, ADG, and EBI compared to the other treatments (p<0.05). Chickens fed with diets including various levels of amaranth grain showed the decreased low-density lipoprotein (LDL) and cholesterol concentrations in the blood sera and reduced relative weight of abdominal fat compared to the control (p<0.05). Dietary addition of amaranth grain up to the level of 2% could improve the performance of broiler chickens, decreased blood cholesterol and LDL levels, and relative weight of abdominal fat which may have healthful effects on the birds and broiler-meat-consumers

Comparison of Different Herbal Additives on Immune Response and Growth Performance of Broiler Chickens

This experiment was conducted to compare the effects of four commercial herbal additives (Noviherb®, Bioessence®, Biostrong®, and Novigrow®), and a commercial antibiotic (virginiamycin) on growth performance, carcass yield, visceral organs weight, thyroid hormones, and humoral immune responses of broiler chickens. Nine hundred day-old Ross 308 male broiler chicks were reared in litter-covered floor cages and distributed into five experimental groups with six replicates of 30 birds. Five dietary treatments tested were diets supplemented with Noviherb®, Bioessence®, Biostrong® (each of them 100g/ton of diet), Novigrow® (1000g/ton of diet), and virginiamycin (100g/ton of diet) as a positive control. Feed intake, body weight, mortality, feed conversion ratio, European broiler index, and feed cost per kilogram of body weight were measured during the experiment. Antibody titers against sheep red blood cells (SRBC), immunoglobulin G, immunoglobulin M, and plasma concentrations of thyroxine (T4) and triiodothyronine (T3) were evaluated at the age of 42 days. Then, four birds per replicate were killed to determine the relative weight of carcass and organs. The GLM procedures of SAS software and Duncan’s multiple range test were applied to analyze data in a completely randomized design with five treatments and six replicates of 30 birds per each. The effects of herbal additives on productive traits feed cost per kilogram of body weight and relative weight of organs were not significant in comparison with antibiotic treatment. Dietary inclusion of Noviherb®, Biostrong®, and Novigrow® significantly decreased feed cost per kilogram of carcass compared with Bioessence® or antibiotic treatments (p<0.05). The titers of IgG in Noviherb® and virginiamycin were significantly higher (p<0.05) than in Biostrong® and Novigrow® fed groups. The plasma concentration of triiodothyronine was significantly decreased (p<0.05) by dietary inclusion of Noviherb® and Novigrow® compared to the antibiotic. In conclusion, all the evaluated herbal additives could act as a substitute for the growth-promoting antibiotic

Bioavailability of rectal acetaminophen in children following anorectal surgery

Background: Acetaminophen is widely used as an analgesic and antipyretic agent in pediatrics. Although bioavailability of rectal acetaminophen is unpredictable, rectal route is a usual and acceptable method of prescription. Major anorectal surgery may alter the normal structure of the surgical site, especially the vascular elements and the normal connections between port and systemic vessels. As a result the pharmacokinetics of rectal medications might also be altered. Based on this hypothesis, we decided to study acetaminophen plasma concentration among children who underwent these types of surgeries to determine the pharmacokinetic of absorption, plasma concentration, safety, and efficacy of rectal acetaminophen. Materials and methods: The study included 20 cases with previous history of pull-through procedure owing to Hirschsprung's disease (HD), 20 cases with imperforate anus (IA) reconstructive surgeries who were admitted for colostomy closure, and 20 otherwise healthy cases of inguinal herniotomy. Venus blood sampling was done 4, 8 and 12 hrs after a single loading dose of rectal acetaminophen (40 mg/kg), and plasma acetaminophen concentration was compared between groups. Results: Mean serum acetaminophen levels of the HD group were significantly higher than those of the herniotomy group (36.3 ± 6.79, 27.4 ± 8.42, 16.8 ± 7.62 versus 25.9 ± 9.12, 16.7 ± 6.74, 8.1 ± 5.79 (μg/ml) at 4, 8 and 12 hrs after drug administration and P < 0.05). The IA group had higher concentrations of plasma acetaminophen compared to the herniotomy group; however, the p values were not statistically significant. (31.4 ± 10.39, 21.5 ± 9.12, 13.3 ± 6.79 versus 25.9 ± 9.12, 16.7 ± 6.74, 8.1 ± 5.79 (μg/ml) at 4, 8 and 12 hrs after drug administration). Serum concentrations of acetaminophen in IA and HD patients were above the therapeutic range four hours after administering the loading dose (31.4 ± 10.39 and 36.3 ± 6.79 versus 5�20 μg/ml). Conclusion: Bioavailability of rectal acetaminophen might get altered after major anorectal surgery in children. Rectal acetaminophen should be administered with special caution among infants with history of anorectal operations. Repeated dose of rectal acetaminophen may cause the drug blood concentration to reach toxic levels in these patients. Type of study: Prospective comparative study. Level of evidence: Level II. © 2020 Elsevier Inc