Effects of Tourism on Subjective Dimensions of Quality of Life: The Case of Tourist Destination Villages of Marvdasht County

This research aimed at investigating the impacts of tourism on residents' subjective quality of life in tourist destination villages of Marvdasht County, Iran. The statistical population included 170 heads of households living in the tourist destination villages of Marvdasht County, selected by the simple randomization method and based on Cochran’s formula. The data collection instrument was a researcher-made questionnaire whose validity and reliability was confirmed. Data were analyzed by exploratory factor analysis and Morris’s model. The results of factor analysis showed that the components including cost, local livelihood, and asset accounted for 61.27, 58.77, and 58.06 percent of the total variance in the economic dimension of subjective quality of life, respectively. Social dimension components (including local community and nutrition and health) also captured 66.17 and 60.48 percent and environmental dimension components (including environmental sustainability and physical dimensions) captured 61.28 and 67.87 percent of the total variance in residents' subjective quality of life, respectively. According to the findings, the level of subjective quality of life was medium (with the coefficient of 0.55) based on the Morris method. While the economic (with a coefficient of 0.53) and environmental (with a coefficient of 0.54) dimensions of subjective quality of life were at the medium level, the social dimensions of subjective quality of life (with a coefficient of 0.6) were at the favorable level. Therefore, the highest score of the subjective quality of life in the tourist destination villages was related to the social index. These findings can be used to identify previous policy strategies and design future planning policies

Nano-Microbial Remediation of Polluted Soil: A Brief Insight

The pollution of soil by heavy metals and organic pollutants has become a significant issue in recent decades. For the last few years, nanobiotechnology has been used to bio-remediate or reclaim soil contaminated with organic and inorganic pollutants. The removal of pollutants from industrial wastes is a major challenge. The utilization of nanomaterials is gaining popularity, which might be accredited to their enhanced physical, chemical, and mechanical qualities. The development of advanced nanobiotechnological techniques involving the use of nanomaterials for the reclamation of polluted soils has indicated promising results and future hope for sustainable agriculture. By manufacturing environment-friendly nanomaterials, the industrial expenditure on decreasing the load of pollution might be reduced. A potential emerging domain of nanotechnology for eco-friendly production and cost reduction is “green biotechnology”, alongside the utilization of microorganisms in nanoparticle synthesis

Self-Organized Cerebral Organoids with Human-Specific Features Predict Effective Drugs to Combat Zika Virus Infection

Summary: The human cerebral cortex possesses distinct structural and functional features that are not found in the lower species traditionally used to model brain development and disease. Accordingly, considerable attention has been placed on the development of methods to direct pluripotent stem cells to form human brain-like structures termed organoids. However, many organoid differentiation protocols are inefficient and display marked variability in their ability to recapitulate the three-dimensional architecture and course of neurogenesis in the developing human brain. Here, we describe optimized organoid culture methods that efficiently and reliably produce cortical and basal ganglia structures similar to those in the human fetal brain in vivo. Neurons within the organoids are functional and exhibit network-like activities. We further demonstrate the utility of this organoid system for modeling the teratogenic effects of Zika virus on the developing brain and identifying more susceptibility receptors and therapeutic compounds that can mitigate its destructive actions. : Cerebral organoids recapitulate many aspects of human corticogenesis and are a useful platform for modeling neurodevelopmental mechanisms and diseases. Watanabe et al. describe enhanced organoid methods and model ZIKV pathology. More susceptibility receptors for ZIKV are identified, and differential effects of various compounds to mitigate ZIKV-induced cytopathy are demonstrated. Keywords: neurogenesis, neural stem cell, embryonic stem cell, organoid, differentiation, neural development, cerebral cortex, Zika virus, human brai

Self-Organized Cerebral Organoids with Human-Specific Features Predict Effective Drugs to Combat Zika Virus Infection.

The human cerebral cortex possesses distinct structural and functional features that are not found in the lower species traditionally used to model brain development and disease. Accordingly, considerable attention has been placed on the development of methods to direct pluripotent stem cells to form human brain-like structures termed organoids. However, many organoid differentiation protocols are inefficient and display marked variability in their ability to recapitulate the three-dimensional architecture and course of neurogenesis in the developing human brain. Here, we describe optimized organoid culture methods that efficiently and reliably produce cortical and basal ganglia structures similar to those in the human fetal brain in vivo. Neurons within the organoids are functional and exhibit network-like activities. We further demonstrate the utility of this organoid system for modeling the teratogenic effects of Zika virus on the developing brain and identifying more susceptibility receptors and therapeutic compounds that can mitigate its destructive actions

25-Hydroxycholesterol Protects Host against Zika Virus Infection and Its Associated Microcephaly in a Mouse Model

Zika virus (ZIKV) has become a public health threat due to its global transmission and link to severe congenital disorders. The host immune responses to ZIKV infection have not been fully elucidated, and effective therapeutics are not currently available. Herein, we demonstrated that cholesterol-25-hydroxylase (CH25H) was induced in response to ZIKV infection and that its enzymatic product, 25-hydroxycholesterol (25HC), was a critical mediator of host protection against ZIKV. Synthetic 25HC addition inhibited ZIKV infection in vitro by blocking viral entry, and treatment with 25HC reduced viremia and conferred protection against ZIKV in mice and rhesus macaques. 25HC suppressed ZIKV infection and reduced tissue damage in human cortical organoids and the embryonic brain of the ZIKV-induced mouse microcephaly model. Our findings highlight the protective role of CH25H during ZIKV infection and the potential use of 25HC as a natural antiviral agent to combat ZIKV infection and prevent ZIKV-associated outcomes, such as microcephaly