The number of transcription factor binding sites per proximal promoter is higher on autosomes than on sex chromosomes.

<p>This figure consists of two parts identified as <i>a</i> and <i>b</i>. In part <i>a</i>, the average number of transcription factor binding sites per promoter in symmetrical windows around transcriptional start sites (TSSes) is shown. The plots have a characteristic shape of the V-sign. On the <i>x</i>-axis of panel <i>a</i>, values from negative 3 kbps to zero signify positions upstream TSSes (negative values signify positions downstream the TSS). In part <i>b</i>, V-sign-shaped curves are plotted separately for each chromosome (and the <i>x</i>-axis corresponds to the order of chromosomes from 1 to 22 plus the X and Y). The curves are similar between autosomes, but TfbsNo is lower for sex chromosomes. The underlying data can be found at <a href="http://fantom.gsc.riken.jp/5/data/" target="_blank">http://fantom.gsc.riken.jp/5/data/</a> and in Dryad Digital Repository (doi:<a href="http://dx.doi.org/10.5061/dryad.p4s57" target="_blank">10.5061/dryad.p4s57</a>).</p

A correlation between tissue-specific maximal expression (TSME) and binary enrichment on the X chromosome.

<p>This figure shows a scatterplot where each data point is a FANTOM5 library (points are colored-coded to highlight brain tissues, sex-specific tissues, and the placenta). <i>X</i>-axis corresponds to the average maximal expression of given tissue’s top 0.1% preferentially expressed genes (i.e., TSME, using a logarithmic scale). <i>Y</i>-axis corresponds to binary enrichment. The strength of the Spearman correlation and <i>p-</i>values are annotated with text above the figure panel. Data points that have standardized residuals more than 1.96 standard deviations (highlighted as grey area) from the linear regression line (which is plotted in black) have their names annotated with text. The underlying data can be found at <a href="http://fantom.gsc.riken.jp/5/data/" target="_blank">http://fantom.gsc.riken.jp/5/data/</a> and in Dryad Digital Repository (doi:<a href="http://dx.doi.org/10.5061/dryad.p4s57" target="_blank">10.5061/dryad.p4s57</a>).</p

The comparison of change in gene expression (Z) since the human-Chimpanzee common ancestor for five somatic tissues.

<p>Genes are divided into X-linked (yellow) and autosomal (green). In turn, they are split into a half with low expression in the ancestor (<i>low</i>) and a half with high expression (<i>high</i>). Genes with no expression in the ancestor are excluded from this analysis (but this makes no qualitative difference). In all instances, the high-expression X-linked genes have a lower median Z-score than high-expression autosomal genes, this being significant in three instances using a Mann Whitney U test (shown as *). The combined <i>p-</i>value is highly significant (see main text). There is no consistent trend for the low-expression genes. The underlying data can be found at <a href="http://fantom.gsc.riken.jp/5/data/" target="_blank">http://fantom.gsc.riken.jp/5/data/</a> and in Dryad Digital Repository (doi:<a href="http://dx.doi.org/10.5061/dryad.p4s57" target="_blank">10.5061/dryad.p4s57</a>).</p

A shift in the breadth of expression for split pairs of autosomal-X paralogs.

<p>This figure shows barplots for the shift in the breadth of expression (ΔBoE) depending on the taxon of duplication for autosomal-X paralogs. The critical result is that all groups except primates were shifted significantly below zero (Wilcoxon one-sided test <i>p-</i>values are given brackets): primate (<i>p</i> = 0.639), mammalian (<i>p</i> = 2.479e-12), vertebrate (<i>p</i> = 9.178e-13), animal (<i>p</i> = 1.046e-07), eukaryotic (<i>p</i> = 0.00087). The differences between groups were not statistically significant after multiple-testing correction, but it was not the point of this analysis to show any differences between the taxa. For figure clarity, we do not show these data, but as expected (as these are non-directional comparisons), the average ΔBoE is close to zero for autosomal-only (same or different chromosome), X-only, and Y-only duplications, regardless of age. The underlying data can be found at <a href="http://fantom.gsc.riken.jp/5/data/" target="_blank">http://fantom.gsc.riken.jp/5/data/</a> and in Dryad Digital Repository (doi:<a href="http://dx.doi.org/10.5061/dryad.p4s57" target="_blank">10.5061/dryad.p4s57</a>).</p

Analog enrichment in expression on the X chromosome.

<p>This figure consists of two panels identifiable as <i>a</i> and <i>b</i>. Each panel shows the ratio of average (<i>per locus</i>) expression on autosomes over that observed on the X chromosome (if the ratio was higher than one given tissue was enriched in expression on autosomes). Panel <i>a</i> shows data for all genes, panel <i>b</i> shows data only for tissue-specific genes (i.e., these with the breadth of expression lower than 0.33). Only the top ten over-represented and the top ten under-represented tissues are shown. Brain subsets are clearly most X-enriched tissues. Exocrine gastrointestinal glands, in contrast, are the most X-depleted tissues. The underlying data can be found at <a href="http://fantom.gsc.riken.jp/5/data/" target="_blank">http://fantom.gsc.riken.jp/5/data/</a> and in Dryad Digital Repository (doi:<a href="http://dx.doi.org/10.5061/dryad.p4s57" target="_blank">10.5061/dryad.p4s57</a>).</p

A shift towards a lower breadth of expression on the X is duplication-dependent.

<p>This figure shows boxplots for the breadth of expression depending on the chromosomal location and gene family size. Only for medium (more than two members) and big gene families (more than five members) is there a difference in the breadth of expression between autosomes and the X, suggesting the effect is duplication-dependent. The underlying data can be found at <a href="http://fantom.gsc.riken.jp/5/data/" target="_blank">http://fantom.gsc.riken.jp/5/data/</a> and in Dryad Digital Repository (doi:<a href="http://dx.doi.org/10.5061/dryad.p4s57" target="_blank">10.5061/dryad.p4s57</a>).</p

A lower maximal expression level on the X chromosome.

<p>This figure shows maximal expression levels for autosomes and the X chromosome. Maximal expression is defined as transcript’s maximal expression level (in TPM) in any of the FANTOM5 human tissues. The underlying data can be found at <a href="http://fantom.gsc.riken.jp/5/data/" target="_blank">http://fantom.gsc.riken.jp/5/data/</a> and in Dryad Digital Repository (doi:<a href="http://dx.doi.org/10.5061/dryad.p4s57" target="_blank">10.5061/dryad.p4s57</a>) [<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1002315#pbio.1002315.ref043" target="_blank">43</a>].</p

human.cell_line.hCAGE.hg19.tpm.refgene.osc

The FANTOM5 dataset for human cancer cell-lines

chicken.all_samples.galGal3.tpm.refgene.osc

Data for the analysis of the chicken chromosome Z. FANTOM5 chicken libraries consisted of 25 CAGE libraries including: chicken aortic smooth muscles, hepatocytes, mesenchymal stem cells, leg buds, wing buds, embryo extra-embryonic tissue (day 7 and day 15), and whole body developmental time course (from 5 hours 30 minutes to 20 days). The number of available datapoints to which TPM was normalized was limited by the number of annotated chicken RefSeq transcripts (which was approximately six times smaller than human, N = 4,426 on autosomes, and N = 241 on chromosome Z). Consequently, the cutoff for a gene to be classified as “on” was adjusted six times higher to 60 TPM

Additional TreeFam gene duplication data with duplication timing

Additional TreeFam gene duplication data with duplication timin