Porównanie czynników ryzyka niekorzystnych wyników położniczych u młodocianych ciężarnych i ciężarnych w zaawansowanym wieku

Objectives: To evaluate clinical outcomes of pregnancies in adolescent, advanced and reproductive women maternal age. Materials and methods: A total of 187 pregnant women were included into the study, 51 (27.27 %) were adolescent pregnancies, mean age 17.7±3.3 years (range, 14-18 years), 40 (21.29 %) advanced maternal age pregnancies, mean age 41.4±2.6 years (range, 39-50 years) and 96 (51.33 %), healthy controls, mean age 28.8±4.3 years (range, 19-37 years). The majority of the adolescent pregnant women were nulliparous, while all of the advanced age pregnant women were multiparous (mean gravidity 4.5±2.4; range, 6-12). Mean gravidity of the control group was 3.6± 1.7 (range, 1-12). Results: There was statistically significant difference among the groups regarding demographic characteristics. According to the Bonferroni method, there was statistically significant difference among the three groups. Adverse fetal features were statistically different among the groups and the odd ratios were higher in the advanced maternal age group. The odds ratios (95% CI) for lower Apgar scores (Apgar score 1 and 5) and low birth weight in adolescent maternal age and advanced maternal age group were as follows: 1.88 (1.27 - 2.78) vs. 2.46 (1.55 - 3.85), 2.17 (1.18 - 4.00) vs. 4.79 (3.02-6.69) and 1.39 (1.14 - 1.68) vs. 3.06 (1.59 - 5.88), respectively. Gestational age at birth showed minimal risk for adolescent age group but no risk was noted in the advanced age group. Conclusions: The present study showed that pregnancies in adolescent maternal age, advanced maternal age and reproductive maternal age are different from each other in terms of clinical characteristics. According to this study advanced maternal age pregnancies are more risky and more likely to have adverse fetal outcome.Cel: Ocena wyników położniczych u ciężarnych młodocianych oraz w wieku zaawansowanym i reprodukcyjnym. Materiał i metody: Do badania włączono 187 ciężarnych, 51 (27,27%) młodocianych, średnia wieku 17,7±3,3 lat (zakres 14-18 lat), 40 (21,29%) w zaawansowanym wieku, średnia wieku 41,4±2,6 lat (zakres 39-50 lat) i 96 (51,33%) zdrowych ciężarnych stanowiących grupę kontrolną, średnia wieku 28,8±4,3 lat (zakres 19-37 lat). Większość młodocianych ciężarnych była nieródkami, podczas gdy wszystkie starsze ciężarne były wieloródkami (średnia ilość ciąż 4,5±2,4; zakres 6-12). Średnia rodność w grupie kontrolnej wynosiła 3,6±1,7 (zakres 1-12). Wyniki: Znaleziono istotną statystycznie różnicę pomiędzy grupami dotyczącą cech demograficznych. Według metody Bonferroni różnica pomiędzy trzema badanymi grupami była istotna statystycznie. Niekorzystne wyniki położnicze oraz iloraz szans były wyższe u kobiet ciężarnych w zaawansowanym wieku. Ilorazy szans (95%CI) dla niższego wyniku w skali Agar (Agar 1 i 5) oraz niższej masy urodzeniowej w grupie młodocianych i grupie starszych ciężarnych wynosiły odpowiednio: 1,88 (1,27-2,78) vs. 2,46(1,55-3,85), 2,17(1,18-4,00) vs. 4,79 (3,02-6,69) i 1,39 ((1,14-1,68) vs. 3,06 (1,59-5,88). Wiek ciążowy w momencie porodu okazał się minimalnie istotnym czynnikiem ryzyka w grupie młodocianych a w grupie starszych ciężarnych nie obserwowano żadnego ryzyka. Wnioski: Badanie wykazało, że ciąże u młodocianych, u kobiet w starszym wieku oraz w wieku rozrodczym różnią się między sobą pod względem klinicznym. Według naszego badania ciąża u kobiety w starszym wieku jest obarczona większym ryzykiem niekorzystnych wyników położniczych

Poziom antyoksydantów a wymioty ciężarnych – badanie porównawcze przypadków

Objectives: Dietary antioxidant intake decreases the risk of many diseases. Hyperemesis gravidarum (HG) is the most common eating disorder during pregnancy. Therefore, the authors conducted this prospective and case control study to evaluate dietary antioxidant levels of women with HG and compare with healthy pregnant women. Material and Methods: This prospective case control study was conducted at a government hospital in the southeastern region of Turkey, from February 2010 to May 2010. A total of 100 pregnant women were included into the study. Dietary antioxidant levels (DAL) were measured according to the new 92-item antioxidant nutrient questionnaire developed by Satia et al (1). 50 women with HG and 50 healthy pregnant women were evaluated. Statistical analyses were carried out with statistical packages for SPSS 15.0 for Windows (SPSS Inc., Chicago, IL, USA). Results: There were no statistically significant differences between the groups regarding the age of the patients, gestational age, educational status, body height and weight (p>.05). Vitamin E, E equivalent, vitamin C, carotene and vitamin A levels were significantly lower in women with HG (pCel: Spożywanie antyoksydantów obniża ryzyko wielu chorób. Wymioty ciężarnych (HG) są najczęstszym zaburzeniem odżywiania w ciąży. Z tego powodu autorzy przeprowadzili badanie porównawcze przypadków celem oceny poziomu antyoksydantów u kobiet z wymiotami ciężarnych w porównaniu ze zdrowymi kobietami. Materiał i metody: Badanie przeprowadzono w szpitalu w południowowschodniej Turcji, w okresie od lutego 2010 do maja 2010 na 100 ciężarnych kobietach. Poziom antyoksydantów (DAL) mierzono według nowego 92-pytaniowego kwestionariusza dotyczącego spożycia antyoksydantów opracowanego przez Satia [1]. Oceniono 50 ciężarnych z wymiotami i 50 zdrowych ciężarnych. Analizę statystyczną przeprowadzono przy pomocy pakietu statystycznego SPSS 15.0 dla Windows (SPSS Inc., Chicago, IL, USA). Wyniki: Nie znaleziono istotnych statystycznie różnic pomiędzy badanymi grupami w odniesieniu do wieku pacjentek, wieku ciążowego, wykształcenia, wzrostu i wagi (p>0,05). Poziomy witaminy E, równoważnika wit. E, witaminy C, karotenu, i witaminy A były istotnie niższe u ciężarnych z wymiotami (

Maternal serum amyloid A levels in pregnancies complicated with preterm prelabour rupture of membranes

Objective: The aim of the study was to investigate a possible association between maternal serum amyloid A levels (SAA) and maternal and fetal parameters in pregnancies complicated with preterm prelabor rupture of membranes (PPROM). Material and methods: A total of 88 pregnant women (PPROM group, n=44 and control group, n=44) were included into this prospective case control study. Serum blood samples for SAA were obtained from both groups within 1h since the rupture of the membranes and before administration of any medicine. The samples were kept frozen at -70°C until the analysis. The recorded risk factors were: age, gravidity, parity, delivery mode, gender, fetal birth weight, APGAR scores, white blood cell count, microCRP, neutrophil/lymphocyte ratio (NLR), and maternal serum SAA levels. Results: Demographic characteristics showed no statistically significant differences between the groups (p>0.05). The mode of delivery mode was cesarean section: 41% and 43.2% in the study and the control group, respectively, and this difference was statistically significant between the groups (

Chronic Myeloid Leukemia In a Pregnant Woman: A Case Report

Chronic myeloid leukemia (CML) is a rare disease in pregnancy. Our aim is to present a 37 weeks of pregnant woman with chronic myelogenous leukemia. A 27 Years in multigravi (gravida 5, parity: 4), at 37 weeks gestation was admitted with the diagnosis of painful pregnancy and CML. Physical examination findings were normal, complete blood count and peripheral blood smear results were consistent with CML. The patient was diagnosed CML in the 30th week of pregnancy and were treated with hydroxyurea and interferon. Treatment depends on the mother and the fetus did not develop any side effects. Our patient with CML is interesting due to lack of perinatal effects and take the diagnosis at an early age. CML diagnosed during pregnancy requires a multidisciplinary approach and hydroxyurea and interferon treatment on the mother and fetus are at low risk of inducing adverse effects. [Cukurova Med J 2015; 40(4.000): 811-813

Spinal Muscular Atrophy and Its Molecular Genetics

Spinal muscular atrophy (SMA) is one of the most common autosomal recessive diseases, affecting aproximately 1 in 6,000 - 10,000 live births, and with a carrier frequency of aproximately 1 in 40- 60. The childhood SMAs can be classified clinically into three groups. Type I (Werdnig-Hoffmann) is the most severe form, with onset at ‹ 6 months of age and with death typically at ‹2 years of age. Type II SMA patients display an intermediate severity, with onset at ‹18 months of age and with an inability to walk. Type III (Kugelberg –Walender) individuals are able to walk independently and have a relatively mild phenotype, with onset at ›18 months of age. The gene involved in type I–III SMA has been mapped to 5q12-q13 by linkage analysis, and refined to a region of about 500 kb. The region contains a large inverted duplication consisting of at least four genes, which are present in a telomeric (t) and a centromeric (c) copy: survival motor neuron gene (SMN1 or SMNt and SMN2 or SMNc); neuronal apoptosis inhibitory protein gene (NAIP); basal transcription factor subunit p44 (BTFp44t and BTFp44c); and a novel protein with unknown function H4F5. Although homozygous deletions encompassing all these genes are found in SMA patients, it is now well established that mutations or deletions of SMN1 (MIM#600354) cause the disease. SMN2 (MIM# 601627) gene, however, does not prevent the disease but attenuates disease severity. Therefore, upregulating functional SMN protein level via inducing gene expression and/or restoring splicing is an important therapeutic approach such as use of histone deacetylase (HDAC) inhibitors. [Archives Medical Review Journal 2012; 21(1.000): 1-26

Cloning and Expression of Cellulosimicrobium cellulans beta-1,3-Glucanase Gene in Lactobacillus plantarum to Create New Silage Inoculant for Aerobic Stability

WOS: 000321751900004In this study, the recombinant plasmid pTE353-beta G was created by inserting the p353-2 cryptic plasmid region of pLP3537 into pTEG5 recombinant plasmid contains pUC18 and beta-1,3-glucanase gene of Cellulosimicrobium cellulans. The recombinant plasmid pTE353-beta G was then introduced into Lactobacillus plantarum by electroporation. Insert analysis of pTE353-beta G digested with SacI produced 1.9 kbp beta-1,3-glucanase gene band on agarose gel as well as 1.9 kbp DNA encoding beta-1,3-glucanase gene insert amplified on the recombinant vector via PCR indicated the integration of the gene into the plasmid. Recombinant L. plantarum colonies with pTE353-beta G on MRS-laminarin-agar plate showed clear positive zones by Congo-red staining that revealed the expression of beta-1,3-glucanase encoding gene. The beta-1,3-glucanase enzyme of recombinant strain produced the same activity band with C. cellulans enzyme in terms of molecular weight, which showed the activity of secreted protein without any proteolytic degradation. Optimal temperature and pH values of L. plantarum beta-1,3-glucanase have been determined 40 degrees C and 6.0 respectively, by enzymatic analysis. These results revealed that recombinant L. plantarum could be considered as a silage inoculant for aerobic spoilage of silage

Cloning and Expression of beta-1,3-Glucanase Gene from Cellulosimicrobium cellulans in Escherichia coli DH5a

WOS: 000321750600026In this study, beta-1,3-glucanase gene of Cellulosimicrobium cellulans was amplified by PCR and cloned in pUC18 cloning vector to construct the recombinant plasmids pTEG5 and pTEG11. The recombinant plasmids pTEG5 and pTEG11 were transformed into competent Escherichia coli cells. Digestion of recombinant plasmids with SacI produced 1.9 kbp beta-1,3-glucanase gene band on agarose gel which indicated the gene integration. a-1,3-Glucanase gene amplification on the recombinant vectors also indicated 1.9 kbp gene insert. Recombinant enzyme was produced by E. coli intracellularly. Intracellular components of recombinant E. coli strains with pTEG5 or pTEG11 dropped on LB-laminarin-agar plate, showed clear positive zones by Congo-red staining revealing the activity of secreted protein. Based on the zymogram analysis, the intracellular produced recombinant beta-1,3-glucanase enzymes exhibited the same activity bands with C. cellulans enzyme with respect to molecular weight.Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [108T919]This study was supported by The Scientific and Technological Research Council of Turkey (TUBITAK), Project No: 108T91

Factors affecting pregnancy rates in infertile women performed abdominal myomectomy

Purpose:To evaluate pregnancy outcomes in infertile women performed abdominal myomectomy at our clinic. Patients and Methods:This retrospective study included 76 infertile women underwent abdominal myomectomy. The cases were divided into two groups according to postoperative pregnancy (Group 1, n=22), and cases with no postoperative pregnancy (Group 2, n=54). Risk factors recorded were; age, parity, size of the fibroids, body mass index (BMI), tumor markers and serum blood values. Results:A total of 76 infertile women underwent abdominal myomectomy during the study period. Of all cases 22 (28.94 %) became pregnant. There was statically significant difference between the groups in terms of age, BMI, diameter of the fibroids (p<0.05) (Table 2). The receiver operator curve (ROC) analyses showed that diameter of the fibroid may be a prognostic factor in order to assess the probability of pregnancy following abdominal myomectomy in infertile women. Conclusion:We think that in infertile women with intramural fibroids >5 cm the treatment modality should be abdominal myomectomy to increase the chance of postoperative pregnancy. [Cukurova Med J 2014; 39(4.000): 801-806

Variants in taste genes on caries risk and caries activity status

WOS: 000557110000001PubMed: 32770263The aim of this study was to evaluate the role of taste-related gene polymorphisms (CA6,TAS1R1,TAS1R3, TLR2,andTLR4)on dental caries and caries activity in adults. Individuals aged 25-44 years included in the study were assigned to two groups according to the decayed-missing-filled teeth index (DMFT) as the high caries risk (DMFT >= 14,n = 100) and the low caries risk (DMFT <= 5,n = 100). TaqMan allelic discrimination assays were used for genotyping the gene variants after isolating the DNA from the buccal smears. According to the American dental association caries classification system (ADA CCS), all teeth were scored as initial, moderate or advanced caries. the variant of the gustin (CA6) in saliva was found to be associated with a high caries risk (CA6rs17032907,P < .001). There was also a statistically significant difference in the dominant model of the same variant (CC vs. TT:P < .001, OR = 5.05, 95% CI: 2.38-10.71). the presence of genotype CC and allele C was less frequent in the advanced caries lesion group (P < .001). This study shows that theCA6rs17032907 gene variant may be a risk factor for dental caries affecting caries activity. Clinical Trials ID: NCT04066101.Recep Tayyip Erdogan University Scientific Research Projects Foundation [TSA-2017-772]The study was supported by the Recep Tayyip Erdogan University Scientific Research Projects Foundation (TSA-2017-772)