Relationships Among Some Biochemical Parameters, Trace Elements and Lipid Peroxidation Levels in Women With Gestational Diabetes Mellitus

Aim:The aim of the present study was to determine levels of copper (Cu), zinc (Zn), iron (Fe), malondialdehyde and some biochemical parameters in women with gestational diabetes mellitus (GDM) compared with healthy pregnant women, and to evaluate the relationships among these parameters.Materials and Methods:The patients consisted of 56 pregnant with GDM. The control group consisted of 60 healthy pregnant women. Plasma malondialdehyde, blood glucose, insulin, glycosylated hemoglobin (HbA1c), total cholesterol, triacylglycerol and LDL-cholesterol were measured by biochemical methods. The concentrations of serum Fe, Cu and Zn were determined by flame atomic absorption spectrophotometer.Results:The levels of blood glucose, insulin, HOMA-IR, glycosylated hemoglobin (HbA1c), total cholesterol, triacylglycerol and LDL-cholesterol, levels of plasma malondialdehyde, levels of serum Fe and Cu in women with GDM group were found higher than those in healthy pregnant women. However, levels of serum Zn were significantly lower in GDM pregnants than those in the healthy pregnant women. There were significant negative correlations between LDL-cholesterol and Cu levels of GDM subjects. Additionally, there were significant negative correlation between LDL-cholesterol and plasma malondialdehyde levels. However, there were significant positive correlations between serum Fe and plasma malondialdehyde levels.Conclusion:Our findings emphasize the significant deficiencies of Zn levels and the significant elevation of malondialdehyde, hyperglycemia and hyperlipidemia in women with GDM. Therefore, supplementation with Zn element involved in the antioxidative system may increase antioxidative enzyme activities, and consequently, an improvement in fetal and maternal complications may be expected

Role of spiramycin in prevention of fetal toxoplasmosis

WOS: 000374773800006PubMed ID: 26365472Objective: The aim of this study is to evaluate the efficacy of spiramycin in prevention of mother-to-child transmission of Toxoplasma gondii infection. Methods: Patients within first trimester of their pregnancy with Toxoplasma IgM positivity (>0.65 index, ELISA, VIDAS) and IgG positivity (>8 IU/ml), who had low IgG avidity (<0.50 index, ELISA, Architet) were considered as having acute toxoplasmosis. These patients who had amniocentesis at the 19th-21st week of pregnancy were examined for the detection of Toxoplasma DNA. Detailed ultrasonographic examinations performed between the 20th and 24th gestational weeks and the mothers and babies were followed for at least one year. Results: Out of 61 patients, 55 (90.2%) had received Spy prophylaxis while 6 (9.8%) cases refused Spy prophylaxis. Toxoplasma PCR test was found to be positive in amniotic fluid of 4 (6.6%) patients obtained by amniocentesis at the 19th-21st week of pregnancy. All four of these patients had refused Spy prophylaxis had positive Toxoplasma PCR in amniotic fluid (p<0.01). Conclusion: Our results seem to encourage the use of spiramycin in women with toxoplasmosis during pregnancy

Intussusception Caused by an Appendiceal Mucocele: Case Report

Intussusception of the appendix in to the caecum caused by an appendiceal mucocele is a rarecondition. Acute appendicitis is the most common presentation is of the disease. Radiologic examination methods especially computed tomography can be useful for preoperative diagnosis. If a mucocele is more than 2 cm in size or caused intessuception, right hemicolectomy should be considered

Video1_Can the Pfannenstiel skin incision length be adjusted according to the fetal head during elective cesarean delivery?.mp4

ObjectiveThe study aims to determine whether the Pfannenstiel skin incision can be adjusted according to the fetal head's occipitofrontal diameter (OFD) during primary cesarean delivery.BackgroundEligible 114 nulliparous women delivered at term by cesarean section in which Pfannenstiel skin incision was performed according to the OFD of the fetal head between June 2017 and September 2021 were included. Excluded cases were non-vertex presentations, all emergency cesarean sections, severe preeclampsia, women in an active phase of the first stage of labor and second stage of labor, placenta previa and low-lying placenta, multiple pregnancies, and uncontrolled gestational diabetes mellitus.ResultsAmong 114 eligible nulliparous women, the mean OFD was 116.1 ± 7.2 (99–138) mm, and the measurement of the Pfannenstiel skin incision length, which was performed according to the OFD was found to be 122.8 ± 9.2 (100–155) mm. The difference between OFD and Pfannenstiel incision kept remained within 10 mm in 90 (82.5.2%), 10–20 mm in 17 (15.5%), and more than 20 mm in two women (1.8%). This technique was successful in 109 (95.6%) out of 114 women without extending the skin incision. In five women, skin incision needed to be extended up to 38 mm. In 10 women (8.7%), the rectus abdominis muscle was cut partially to deliver the fetal head. The mean fetal umbilical artery pH was 7.33 ± 0.05. No neonatal hypoxia was encountered in the study.ConclusionPfannenstiel skin incision can be adjusted according to the OFD with minimal margins of error. This technique may provide better cosmetic results by avoiding unnecessarily prolonged incisions with similar newborn outcomes.Clinical Trial RegistrationClinicaltrials.gov, identifier [NCT05632796].</p

Video2_Can the Pfannenstiel skin incision length be adjusted according to the fetal head during elective cesarean delivery?.mp4

ObjectiveThe study aims to determine whether the Pfannenstiel skin incision can be adjusted according to the fetal head's occipitofrontal diameter (OFD) during primary cesarean delivery.BackgroundEligible 114 nulliparous women delivered at term by cesarean section in which Pfannenstiel skin incision was performed according to the OFD of the fetal head between June 2017 and September 2021 were included. Excluded cases were non-vertex presentations, all emergency cesarean sections, severe preeclampsia, women in an active phase of the first stage of labor and second stage of labor, placenta previa and low-lying placenta, multiple pregnancies, and uncontrolled gestational diabetes mellitus.ResultsAmong 114 eligible nulliparous women, the mean OFD was 116.1 ± 7.2 (99–138) mm, and the measurement of the Pfannenstiel skin incision length, which was performed according to the OFD was found to be 122.8 ± 9.2 (100–155) mm. The difference between OFD and Pfannenstiel incision kept remained within 10 mm in 90 (82.5.2%), 10–20 mm in 17 (15.5%), and more than 20 mm in two women (1.8%). This technique was successful in 109 (95.6%) out of 114 women without extending the skin incision. In five women, skin incision needed to be extended up to 38 mm. In 10 women (8.7%), the rectus abdominis muscle was cut partially to deliver the fetal head. The mean fetal umbilical artery pH was 7.33 ± 0.05. No neonatal hypoxia was encountered in the study.ConclusionPfannenstiel skin incision can be adjusted according to the OFD with minimal margins of error. This technique may provide better cosmetic results by avoiding unnecessarily prolonged incisions with similar newborn outcomes.Clinical Trial RegistrationClinicaltrials.gov, identifier [NCT05632796].</p

LOX-1 gene variants and maternal levels of plasma oxidized LDL and malondialdehyde in patients with gestational diabetes mellitus

Purpose The aim of this study was to investigate the relationships between the maternal levels of oxidized LDL (ox-LDL), lipid peroxidation marker malondialdehyde (MDA) and LOX-1 3'UTR188C/T and K167N single nucleotide polymorphisms in pregnant Turkish women with gestational diabetes mellitus (GDM). Methods 116 pregnant women with GDM and 120 healthy pregnant women from the same geographic region were included in the study. Polymerase chain reaction-based restriction analysis was used to identify 3'UTR188C/T and K167N polymorphisms of the LOX-1 gene. Plasma ox-LDL and MDA levels were determined by enzyme-linked immunosorbent assay and spectrophotometric method in all study subjects, respectively. Results Our results indicated that the distribution of the LOX-1 3'UTR188C/T and K167N genotypes and alleles did not differ significantly among subjects with or without GDM (p> 0.05). TT and NN genotype carriers are associated with some glucose metabolism parameters (p 0.05). According to the combined genotype analysis of LOX-1 3'UTR 188 TT and K167N NN polymorphisms, plasma MDA and ox-LDL levels were significantly different between women with GDM and healthy subjects either with or without combined TT/NN genotype carriers (p< 0.001). Conclusions According to our results, ox-LDL and MDA levels were increased in GDM pregnant women and healthy pregnant women either with or without combined TT/NN genotype carriers, for our Turkish sample, these genotype carriers appear to be related with increased oxidative stress in patients with GDM