Assessment of the physiological and biochemical characterization of a Lactic acid bacterium isolated from chicken faeces in sahelian region

The aim of this work was the examination of the microbiological and technological properties of a lactic acid bacterium (CWBI-B623) isolated from chicken faeces in sahelian region (Burkina Faso). The strain CWBI-B623 is a Gram positive rod, asporogenous, catalase-positive, facultatively anaerobic, non motile and mesophilic. The analysis showed that it produce L(+)-lactic acid via homofermentative pathway and it was able to ferment an important number of the carbohydrates of API 50CHL system. The 16S-rDNAsequence analysis revealed that the isolate was phylogenetically a member of the genus Lactobacillus and formed a subline within the Lactobacillus casei cluster. The minimal inhibitory concentration of CWBI-B623 for bile salts was higher than 1% and the survival rates to acidity with pH 3.0 and pH 2.5 were 26 and 1.2%, respectively. CWBI-B623 is an Lactobacillus casei species based on its physiological and biochemical properties and it could be a good candidate for probiotic formulations.Key words: Lactic acid bacteria, Lactobacillus, probiotic, biodiversity

Safety and efficacy of ruxolitinib in an open-label, multicenter, single-arm phase 3b expanded-access study in patients with myelofibrosis: A snapshot of 1144 patients in the JUMP trial

JUMP is a phase 3b expanded-access trial for patients without access to ruxolitinib outside of a clinical study; it is the largest clinical trial to date in patients with myelofibrosis who have been treated with ruxolitinib. Here, we present safety and efficacy findings from an analysis of 1144 patients with intermediate- or high-risk myelofibrosis, as well as a separate analysis of 163 patients with intermediate-1-risk myelofibrosis – a population of patients not included in the phase 3 COMFORT studies. Consistent with ruxolitinib’s mechanism of action, the most common hematologic adverse events were anemia and thrombocytopenia, but these led to treatment discontinuation in only a few cases. The most common non-hematologic adverse events were primarily grade 1/2 and included diarrhea, pyrexia, fatigue, and asthenia. The rates of infections were low and primarily grade 1/2, and no new or unexpected infections were observed. The majority of patients achieved a ≥50% reduction from baseline in palpable spleen length. Improvements in symptoms were rapid, with approximately half of all patients experiencing clinically significant improvements, as assessed by various quality-of-life questionnaires. The safety and efficacy profile in intermediate-1-risk patients was consistent with that in the overall JUMP population and with that previously reported in intermediate-2- and high-risk patients. Overall, ruxolitinib provided clinically meaningful reductions in spleen length and symptoms in patients with myelofibrosis, including those with intermediate-1-risk disease, with a safety and efficacy profile consistent with that observed in the phase 3 COMFORT studies. This trial was registered as NCT01493414 at ClinicalTrials.gov

ETUDE DE LA DIVERSITE ET DES PROPRIETES TECHNOLOGIQUES DE BACTERIES ACIDIFIANTES ORIGINAIRES DU BURKINA FASO

BAYANE Ali (2005). Study of the diversity and the technological properties of acidifying bacteria originating from Burkina Faso. (PhD thesis in French). Faculté Universitaire des Sciences Agronomiques de Gembloux, Belgium., 191 p., 39 tabl., 43 fig.Abstract: The interest of acidifying bacteria from the genus Bacillus in industrial formulations stems from their functional properties and from their ability to form endospores. This last property enables them to resist to several types of environmental stresses without losing the required functional properties. This work is a contribution to the study of the diversity of these spore forming bacteria able to accumulate lactic acid. From three samples taken in the farms of Burkina Faso seventeen (17) new acidifying bacteria were isolated. Six of these isolates were characterized and identified by an approach combining phenotypical and genetic properties. The analyses showed that all of these new bacteria are different from the known acidifying spores forming bacteria. They produced L (+) lactic acid in homo-fermentive way. Several technological properties were observed in these bacteria, which can favour their use as probiotic. In particular their resistance to physical-chemical conditions of the digestive tract (bile salts and acidity), their fermentative profiles and the production of interesting enzymes (amylases, proteases). The production of biomass in the form of spores was studied with these bacteria taking Bacillus coagulans LMG 6326 as a model. This study confirmed the difficulty for spore forming acidifying bacteria to sporulate in liquid media. The work carried out for optimizing the sporulation showed that the immobilized cultures can favour sporulation. A process for spore production was thus described for Bacillus coagulans on alginate beads. Thus, it is possible to obtain a yield of approximately 80% of sporulation with this procedure whereas a maximum yield of only 1% can be achieved in liquid media. The accumulation of sporulation factors in the extracellular medium was identified as a key mechanism responsible for sporulation in the immobilized cultures. This mechanism refers to the "quorum sensing" phenomenon observed in the sporulation of Bacillus subtilis and Clostridium perfringens.BAYANE Ali (2005). Etude de la diversité et des propriétés technologiques de bactéries acidifiantes originaires du Burkina Faso. (Thèse de Doctorat). Faculté Universitaire des Sciences Agronomiques de Gembloux, Belgique., 191 p., 39 tabl., 43 fig.Résumé : L’intérêt des bactéries acidifiantes du genre Bacillus dans les formulations industrielles réside à la fois dans leurs propriétés fonctionnelles et dans leur capacité à former des endospores. Cette dernière propriété leur permet de supporter plusieurs types de stress environnementaux sans perdre les propriétés fonctionnelles recherchées. Ce travail est une contribution à l’étude de la diversité de ces bactéries sporulantes capable d’accumuler de l’acide lactique. A partir de trois échantillons prélevés dans les fermes du Burkina Faso, dix-sept (17) nouvelles bactéries acidifiantes ont été isolées. Six de ces isolats ont été caractérisés et identifiés par une approche combinant des critères phénotypiques et génétiques. Les analyses ont montré que ces nouvelles bactéries sont toutes différentes des bactéries acidifiantes et sporulantes connues. Elles produisent de l’acide L (+) lactique de manière homo-fermentaire. Plusieurs propriétés technologiques intéressantes ont été observées chez ces bactéries, pouvant favoriser leur utilisation comme probiotiques. Il s’agit notamment de leur résistance aux conditions physicochimiques du tractus digestif (acidité et sels biliaires), de leur profil fermentaire et de leur production d’enzymes intéressantes (amylases, protéases). La production de biomasse sous forme de spores a été étudiée chez ces bactéries en prenant Bacillus coagulans LMG 6326 comme modèle. Cette étude de la sporulation a confirmé les observations antérieures concernant la difficulté pour les bactéries acidifiantes de sporuler dans les cultures liquides. Les travaux réalisés dans le but d’optimiser la sporulation ont montré que les cultures immobilisées sont mieux adaptées à la production de spores. Un schéma de production de spores a ainsi été décrit pour Bacillus coagulans sur billes d’alginate permettant d’obtenir un rendement de sporulation de 80% environ, alors qu’il n’est que de 1% en culture liquide. L’accumulation de facteurs de sporulation dans le milieu extracellulaire a été identifiée comme mécanisme intervenant dans l’optimisation de la sporulation dans les cultures immobilisées. Ce mécanisme fait référence au phénomène de "quorum sensing" observé dans la sporulation de Bacillus subtilis et de Clostridium perfringens

Full Length Research Paper - Assessment of the physiological and biochemical characterization of a Lactic acid bacterium isolated from chicken faeces in sahelian region

The aim of this work was the examination of the microbiological and technological properties of a lactic acid bacterium (CWBI-B623) isolated from chicken faeces in sahelian region (Burkina Faso). The strain CWBI-B623 is a Gram positive rod, asporogenous, catalase-positive, facultatively anaerobic, non motile and mesophilic. The analysis showed that it produce L(+)-lactic acid via homofermentative pathway and it was able to ferment an important number of the carbohydrates of API 50CHL system. The 16S-rDNAsequence analysis revealed that the isolate was phylogenetically a member of the genus Lactobacillus and formed a subline within the Lactobacillus casei cluster. The minimal inhibitory concentration of CWBI-B623 for bile salts was higher than 1% and the survival rates to acidity with pH 3.0 and pH 2.5 were 26 and 1.2%, respectively. CWBI-B623 is an Lactobacillus casei species based on its physiological and biochemical properties and it could be a good candidate for probiotic formulations