8 research outputs found
HGF -rs12536657 and Ocular Biometric Parameters in Hyperopic Children, Emmetropic Adolescents, and Young Adults: A Multicenter Quantitative Trait Study
Introduction. Even though ocular refractive state is highly heritable and under strong genetic control, the identification of susceptibility genes remains a challenge. Several HGF (hepatocyte growth factor) gene variants have been associated with ocular refractive errors and corneal pathology. Purpose. Here, we assess the association of an HGF gene variant, previously reported as associated with hyperopia, and ocular biometric parameters in a multicenter Spanish cohort.
Methods. An observational prospective multicenter cross-sectional study was designed, including a total of 403 unrelated subjects comprising 188 hyperopic children (5 to 17 years) and 2 control groups: 52 emmetropic adolescents (13 to 17 years) and 163 emmetropic young adults (18 to 28 years). Each individual underwent a comprehensive eye examination including cycloplegic refraction, and topographic and ocular biometric analysis. Genomic DNA was extracted from oral swabs. HGF single nucleotide polymorphism (SNP) rs12536657 was genotyped. Genotypic, allelic, and logistic regression analyses were performed comparing the different groups. A quantitative trait association test analyzing several biometric parameters was also performed using generalized estimating equations (GEEs) adjusting for age and gender.
Results. No association between rs12536657 and hyperopia was found through gender-adjusted logistic regression comparing the hyperopic children with either of the two control groups. Significant associations between mean topographic corneal curvature and rs12536657 for G/A (slope = +0.32; CI 95%: 0.04-0.60; p=0.023) and A/A (slope = +0.76; CI 95%: 0.12-1.40; p=0.020) genotypes were observed with the age- and gender-adjusted univariate GEE model. Both flat and steep corneal topographic meridians were also significantly associated with rs12536657 for the G/A and A/A genotypes. No association was found between rs12536657 and any other topographic or biometric measurements.
Conclusions. Our results support a possible role for HGF gene variant rs12536657 in corneal curvature in our population. To our knowledge, this is the first multicenter quantitative trait association study of HGF genotypes and ocular biometric parameters comprising a pediatric cohort
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Safety and Efficacy of the Multitargeted Receptor Kinase Inhibitor Pazopanib in the Treatment of Corneal Neovascularization
Purpose.
To evaluate the safety and efficacy of topical pazopanib in the treatment of corneal neovascularization (CNV).
Methods.
Twenty eyes of 20 patients with stable CNV were enrolled in a prospective, open label, noncomparative study and treated with topical pazopanib 0.5% for 3 weeks, and followed for 12 weeks. The primary endpoint was to determine the tolerability and safety of topical pazopanib in the treatment of CNV defined by the occurrence of ocular and systemic adverse events during the study. The secondary endpoint was to evaluate the effect of topical pazopanib on the reduction of (1) neovascular area (NA), defined as the area of the corneal vessels themselves, (2) invasion area (IA), defined as the fraction of the total cornea into which the vessels extend, (3) vessel length (VL), defined as the mean measurement of the extent of vessels from end to end, and (4) vessel caliber (VC), defined as the mean diameter of the corneal vessels.
Results.
There were no severe adverse events following the use of topical pazopanib. Compared with the baseline visit, NA and VL showed a statistically significant decrease at week 3 (P = 0.02 and 0.01, respectively); and NA, IA, and VL statistically significantly decreased at week 12 (P = 0.03, 0.04, and <0.01, respectively). Visual acuity maintained without changes after the 12 week follow-up.
Conclusions.
This preliminary study suggests that topical treatment with pazopanib 0.5% is safe, well tolerated, and may have a role as an alternative for the treatment of CNV (ClinicalTrials.gov number, NCT01257750)
A new model to allow training and analysis of ocular structures in enucleated porcine eyes
HGF-rs12536657 and ocular biometric parameters in hyperopic children, emmetropic adolescents, and young adults: A multicenter quantitative trait study
Introduction. Even though ocular refractive state is highly heritable and under strong genetic control, the identification of
susceptibility genes remains a challenge. Several HGF (hepatocyte growth factor) gene variants have been associated with
ocular refractive errors and corneal pathology. Purpose. Here, we assess the association of an HGF gene variant, previously
reported as associated with hyperopia, and ocular biometric parameters in a multicenter Spanish cohort. Methods. An
observational prospective multicenter cross-sectional study was designed, including a total of 403 unrelated subjects
comprising 188 hyperopic children (5 to 17 years) and 2 control groups: 52 emmetropic adolescents (13 to 17 years) and 163
emmetropic young adults (18 to 28 years). Each individual underwent a comprehensive eye examination including cycloplegic
refraction, and topographic and ocular biometric analysis. Genomic DNA was extracted from oral swabs. HGF single
nucleotide polymorphism (SNP) rs12536657 was genotyped. Genotypic, allelic, and logistic regression analyses were performed comparing the different groups. A quantitative trait association test analyzing several biometric parameters was also
performed using generalized estimating equations (GEEs) adjusting for age and gender. Results. No association between
rs12536657 and hyperopia was found through gender-adjusted logistic regression comparing the hyperopic children with
either of the two control groups. Significant associations between mean topographic corneal curvature and rs12536657 for G/
A (slope � +0.32; CI 95%: 0.04–0.60; p � 0.023) and A/A (slope � +0.76; CI 95%: 0.12–1.40; p � 0.020) genotypes were
observed with the age- and gender-adjusted univariate GEE model. Both flat and steep corneal topographic meridians were
also significantly associated with rs12536657 for the G/A and A/A genotypes. No association was found between rs12536657
and any other topographic or biometric measurements. Conclusions. Our results support a possible role for HGF gene variant
rs12536657 in corneal curvature in our population. To our knowledge, this is the first multicenter quantitative trait association study of HGF genotypes and ocular biometric parameters comprising a pediatric cohort
HGF-rs12536657 and ocular biometric parameters in hyperopic children, emmetropic adolescents, and young adults: A multicenter quantitative trait study
Introduction. Even though ocular refractive state is highly heritable and under strong genetic control, the identification of
susceptibility genes remains a challenge. Several HGF (hepatocyte growth factor) gene variants have been associated with
ocular refractive errors and corneal pathology. Purpose. Here, we assess the association of an HGF gene variant, previously
reported as associated with hyperopia, and ocular biometric parameters in a multicenter Spanish cohort. Methods. An
observational prospective multicenter cross-sectional study was designed, including a total of 403 unrelated subjects
comprising 188 hyperopic children (5 to 17 years) and 2 control groups: 52 emmetropic adolescents (13 to 17 years) and 163
emmetropic young adults (18 to 28 years). Each individual underwent a comprehensive eye examination including cycloplegic
refraction, and topographic and ocular biometric analysis. Genomic DNA was extracted from oral swabs. HGF single
nucleotide polymorphism (SNP) rs12536657 was genotyped. Genotypic, allelic, and logistic regression analyses were performed comparing the different groups. A quantitative trait association test analyzing several biometric parameters was also
performed using generalized estimating equations (GEEs) adjusting for age and gender. Results. No association between
rs12536657 and hyperopia was found through gender-adjusted logistic regression comparing the hyperopic children with
either of the two control groups. Significant associations between mean topographic corneal curvature and rs12536657 for G/
A (slope � +0.32; CI 95%: 0.04–0.60; p � 0.023) and A/A (slope � +0.76; CI 95%: 0.12–1.40; p � 0.020) genotypes were
observed with the age- and gender-adjusted univariate GEE model. Both flat and steep corneal topographic meridians were
also significantly associated with rs12536657 for the G/A and A/A genotypes. No association was found between rs12536657
and any other topographic or biometric measurements. Conclusions. Our results support a possible role for HGF gene variant
rs12536657 in corneal curvature in our population. To our knowledge, this is the first multicenter quantitative trait association study of HGF genotypes and ocular biometric parameters comprising a pediatric cohort
Piogge interminabili rese in forte calo
Il clima avverso è stato l'elemento chiave dell'annata, con un decremento delle produzioni fino al 30%. E il colpo di grazia potrebbe arrivare dalle micotossine
Speciale orzo: un'annata al limite della perfezione.
L'andamento climatico favorevole ha consentito rese medie che non si registravano dal 2000. Il Nord torna alla ribalta dopo lo stop del 2011. Risultati eccellenti anche in termini di pesi ettolitrici
Speciale orzo: produzioni ottime al Nord e buone al Centro-Sud
Il clima ha consentito una delle migliori stagioni a livello di rese compensando i prezzi bassi. Seconda stagione consecutiva positiva per gli agricoltori che hanno puntato sull’orzo. La produzione media complessiva al nord (33 varieta’ testate in 8 localita’ diverse) ha raggiunto le 7,6 t/ha; nel centro Italia, le 32 varieta’ testate nelle 6 località hanno registrato una media di 6,7 t/ha; infine, il sud ha registrato una rea media decisamente buona (5 t/ha) e superiore allo scorso anno. Inoltre, quest’anno l’andamento climatico ha reso conveniente fare orzo da birra anche al centro-nord