Analysis of Matrix Attachment Region in an Experimental Vector, pA205

Recent literature has shown that Matrix and Scaffold Attachment Regions (MARs or SARs) are DNA domains that enhance transcriptional frequency and increase gene expression levels in cells. Research has shown that cell lines containing MARs or SARs are more stable and more productive. Researchers at Abbott Bioresearch Center in Worcester, MA have found two possible MAR regions in an antibody producing cell line. In order to determine whether these regions function as MARs, and if the observed high levels of expression are specific to this cell line, the sequences in question were inserted upstream of the reporter gene, Enhanced Green Fluorescent Protein (EGFP) and tested using FACS analysis. The data indicated the sequences under investigation indeed increased expression of EGFP in stable cell lines, indicating that these sequences can be used to improve transfection and establish high expressing, stable CHO cell lines

Assessment survey and prediction for potential lead contaminated housing in Dorchester.

This project, sponsored by the Environmental Protection Agency New England Office, refined and tested a visual assessment tool for the evaluation of environmental lead in Boston's Dorchester neighborhood. The team surveyed 2,300 homes for the likely presence of lead. A database of the team's result was compared to a database of known lead contaminated properties to test the predictive value of the visual assessment tool. Utilizing GIS mapping technology, the team was able to recommend strategies for achieving the EPA goal of lead safe homes in Dorchester by the 2006 regional deadline